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Combined medicine for treating tumors

A technology for drugs and tumors, applied in the field of combined drugs for the treatment of tumors, can solve problems such as unfavorable industrialized production, long processing cycles, and cumbersome processes, and achieve the effects of significant drug efficacy, good synergy, and clear molecular structure.

Inactive Publication Date: 2015-01-14
楼兰花
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the role of arsenic trioxide in the treatment of solid tumors has been confirmed in many theoretical studies, there has been no significant progress in the clinical treatment of solid tumors.
The existing technological process is cumbersome, the treatment cycle is long, and the yield is low, which is not conducive to industrial production
Moreover, in the prior art, only one of the active components is extracted from the main two active components in hematoxylin, or brazilian hematoxylin, or brazilian hematoxylin
If the two components of brazilian hematoxylin and brazilian hematoxylin are not extracted separately, one of the active components will be lost in the two main components, which will not only affect the effect of the drug, but also affect the quality of the drug due to the extraction process and method. and other issues that affect the yield of the extract

Method used

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  • Combined medicine for treating tumors
  • Combined medicine for treating tumors
  • Combined medicine for treating tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment one: the preparation of arsenic compound solution

[0054] Accurately weigh 1000mg of As with a purity of 99.9% 2 o 3 The powder was poured into a flask filled with 200 ml of sterile deionized water, As 2 o 3 It is a suspension, heated and added dropwise with 4mol / L NaOH until the powder is completely dissolved, then add 750ml of sterile deionized water, then adjust the solution to pH 7.3 with 0.3mol / L NaOH, and make up the volume with sterile deionized water to 1000ml of arsenic compound solution, the concentration is as 2 o 3 Calculated as 1 mg / ml. Prepared As 2 o 3 Solutions were sterilized by filtration (through a 0.22 μm filter) and stored in sterile vials.

Embodiment 2

[0055] Embodiment two: the preparation of Brazilian hematoxylin compound

[0056] 1), the preparation of brazilian hematoxylin

[0057] The pulverized traditional Chinese medicine hematoxylin heartwood is used as raw material, distilled water or deionized water is used as solvent, and the mass ratio of hematoxylin and distilled water or deionized water is 6 times, and extracted at 95°C for 1 hour; extracted 3 times, combined extraction liquid, use 240 mesh sieve or filter screen to filter, filter with medium-speed or fast qualitative filter paper or place it on a Buchner funnel, and use a vacuum pump to filter the hematoxylin extract to obtain the hematoxylin water extract; put the hematoxylin water extract on a rotating Concentrate in an evaporator or on an electric furnace, and concentrate to 1 / 15 of the total volume of the sumac extract to obtain a sumac concentrate; place the concentrate overnight to form a crystal precipitate, separate the upper layer of sumac concentrate...

Embodiment 3

[0060] Embodiment three: adopt the arsenic compound prepared in embodiment one and the hematoxylin water extract prepared in embodiment two step 1) (because the component in the water extract is the described brazilian hematoxylin compound, so carry out with this hematoxylin water extract In vitro experiment) in vitro experiment of inducing tumor cell apoptosis.

[0061] 1. Test material

[0062] 1) Cell lines: T lymphoma cell line Jurkat cells were selected from three cell lines of T lymphoma cell line Jurkat cell line, B lymphoma cell line Daudi cell line and liver cancer cell line HEPG2 for experiment.

[0063] 2) Drug: arsenic compound (the preparation method is: the arsenic compound solution prepared in Example 1 is first diluted to 80 μg / ml with deionized water, and then diluted to the required concentration with 1640 culture solution); step 1 of embodiment two ) made hematoxylin water extract (diluted to the required concentration with 1640 culture medium).

[0064] 2...

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Abstract

The invention discloses a combined medicine for treating tumors. The combined medicine is prepared from unit preparations of an arsenic compound solution and a brazilin compound with different specifications, wherein the arsenic compound solution is prepared by the following steps: adding As2O3 powder into aseptic deionized water to obtain a suspension liquid, and dripping a NaOH solution into the suspension liquid until powder is completely dissolved. The brazilin compound consists of brazilin and brazilein. According to the combined medicine, an arsenic compound and a brazilin compound are combined, so that the curative effect of the arsenic compound can be improved, the dosage and corresponding toxic and side effects of the arsenic compound can be controlled, the combined medicine can be used more safely, and the therapeutic spectrum of the arsenic compound can be extended. The combined medicine has an especially excellent synergetic effect in the aspect of T-lymphoma cell line Jurkat cells.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and relates to the application of an arsenic compound and a brazilian hematoxylin compound in the preparation of a combined medicine for treating tumors, and a combined medicine for treating tumors. Background technique [0002] Arsenic trioxide (As203) is a traditional Chinese medicine arsenic. In the 1970s, Chinese scholars found that it was effective in treating acute promyelocytic leukemia (APL), which aroused extensive attention of researchers and clinicians. However, different tumor cells respond to As 2 o 3 susceptibility varies widely. Such as CN01107536.8 relates to the medicine for the treatment of malignant lymphoma and its preparation method, it discloses that the Raji cell derived from human Burkitt lymphoma cell line has the effect on As 2 o 3 Very sensitive, and Jurkat cells, also derived from human T lymphoma cell line, are sensitive to As 2 o 3 Insensit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/36A61P35/00A61K31/352
CPCA61K31/352A61K33/36A61K2236/15A61K2236/331A61K2236/39A61K2236/51A61K2236/53A61K2236/55
Inventor 楼兰花
Owner 楼兰花
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