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Preparation method of cephalotaxus alkaloid product

A kind of technology of alkaloids and crude torreya, which is applied in the field of preparation of crude torreya alkaloid products, can solve the problems of low yield of crude torreya alkaloids, difficulties in meeting the needs of life, slow growth of tree species, etc., and achieve rapid growth, increased production, and strong metabolism Effect

Inactive Publication Date: 2015-01-21
LUOYANG INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, crude torreya resources are scarce, and the tree species grows slowly, and the output of crude torreya alkaloids is low, which is difficult to meet people's living needs. At the same time, it also causes the price of crude torreya alkaloids to be expensive. Therefore, how to increase the output of crude torreya alkaloids become one of the important issues of people

Method used

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preparation example Construction

[0013] A method for preparing a Torreya alkaloid product. The roots, stems, and leaves of perennial Torreya are selected as explants, cut into strips, sterilized, put into a culture medium to grow symbiotic bacteria, and then obtained from The strains that can metabolize crude torreya alkaloids are isolated from the symbiotic bacteria of torreya torreya, cultivated and fermented, and the fermentation liquid is concentrated and then precipitated to obtain the product. After the activation of the species, put it into an environment with a temperature of 26°C and cultivate it for 48 hours, then inoculate the strains on a petri dish with PDA medium for cultivation, and insert the second batch of strains after 1-2 days after the strains produce metabolic organisms. To promote the growth of the second batch of strains, the second batch of inoculated strains grow out and inoculate into the shaker flasks with fermentation medium, and then put the shaker flasks into a shaker with a rota...

Embodiment 1

[0019] A kind of preparation method of crude torreya alkaloid product, comprises the following steps:

[0020] Step 1. Selection and processing of explants

[0021] Choose the root, stem and leaf of perennial thick torreya as explant, clean the explant with running water, then cut the root into 5-6cm long pieces, remove the old bark and branches and leaves on the stem, and set aside;

[0022] Step 2, sterilization of explants

[0023] Put the treated explants into the ultra-clean workbench, first sterilize them with alcohol with a volume concentration of 75% for 5 minutes, then sterilize them with mercuric chloride with a mass concentration of 0.1% for 12 minutes, wash them with sterile water for 4 times, and put The processed explants were put into sterile water and set aside;

[0024] Step 3, purification and isolation of crude Torreya strain

[0025] Put the sterilized explants into the culture bottles containing the PDA medium respectively, place the culture bottles wit...

Embodiment 2

[0037] A kind of preparation method of crude torreya alkaloid product, comprises the following steps:

[0038] Step 1. Selection and processing of explants

[0039] Choose the root, stem and leaf of perennial thick torreya as explant, clean the explant with running water, then cut the root into 5-6cm long pieces, remove the old bark and branches and leaves on the stem, and set aside;

[0040] Step 2, sterilization of explants

[0041] Put the treated explants into the ultra-clean workbench, first sterilize them with alcohol with a volume concentration of 75% for 5 minutes, then sterilize them with mercuric chloride with a mass concentration of 0.1% for 12 minutes, wash them with sterile water for 5 times, and put The processed explants were put into sterile water and set aside;

[0042] Step 3, purification and isolation of crude Torreya strain

[0043] Put the sterilized explants into the culture bottles containing the PDA medium respectively, place the culture bottles wit...

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Abstract

The invention belongs to the field of plant endophyte separation and microorganism fermentation engineering, and in particular relates to a preparation method of a cephalotaxus alkaloid product. The preparation method comprises the following steps: by adopting roots, stems and leaves of perennial cephalotaxus as explants, cutting into strips, sterilizing, then culturing in a culture medium to grow cephalotaxus symbiotic bacteria, then separating strains capable of metabolizing cephalotaxus alkaloid from the cephalotaxus symbiotic bacteria and performing culture and fermentation, concentrating a fermentation liquid, and then precipitating to prepare a product. According to the preparation method disclosed by the invention, in a preparation process of the fermentation liquid, a second batch of strains are inoculated after the strains generate metabolic organisms, metabolic products of the former batch of the strains are used for providing nutrient substances for the second batch of the strains, so that the growth of the second batch of the strains can be greatly promoted, and the biological activity of the strains also can be improved, and thus a great number of fermentation liquids can be generated, and the yield of cephalotaxus alkaloid can be improved.

Description

technical field [0001] The invention belongs to the field of plant endophyte separation and microbial fermentation engineering, and in particular relates to a method for preparing a crude torreya alkaloid product. Background technique [0002] Torreya is a unique plant in my country, which has high nutritional value and economic value. Torreya alkaloid is one of the important active ingredients of Torreya, which has anti-cancer effects and is often used in medicine. At present, crude torreya resources are scarce, and the tree species grows slowly, and the output of crude torreya alkaloids is low, which is difficult to meet people's living needs. At the same time, it also causes the price of crude torreya alkaloids to be expensive. Therefore, how to increase the output of crude torreya alkaloids become one of the most important issues for people. Contents of the invention [0003] The purpose of the present invention is to provide a method for preparing a crude torreya alk...

Claims

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Application Information

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IPC IPC(8): C12P17/18
Inventor 王祖华王安亭李永程姚战军杨瑞先王佳伟
Owner LUOYANG INST OF SCI & TECH
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