Detection method and kit for human papilloma virus

A technology for virus detection and papilloma, applied in the field of life sciences and methods, can solve the problems of difficult clinical diagnosis, easily polluted supporting consumables, low throughput, etc.

Inactive Publication Date: 2015-01-21
浦艳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0020] The above patents and products use Taqman probes, membrane hybridization technology and suspension chip technology respectively, and there are problems such as low flux or easy pollution or high price of supporting consumables, making it difficult to apply to clinical diagnosis

Method used

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  • Detection method and kit for human papilloma virus
  • Detection method and kit for human papilloma virus
  • Detection method and kit for human papilloma virus

Examples

Experimental program
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Embodiment example

[0047] Human papillomavirus detection kit for testing fresh cervical secretion samples.

[0048] sequence name Nucleotide sequence 5'-3' 6 TTTTAACTACATTCTTCCACATACAC 11 TGTCTAAATCTGCTACATACAC 16 TTTTATTATGTGCTGCCTTATTTACTTC 18 TTTGCTTCTACACAGTCTCCT 31 TTATGTCTGTTTGTGCTGC 33 TATGTCTGTTTGTGCTGC

[0049] 35 TATGCTATTAAAAAACAAGATTCT 39 TTTGTGTTCTGCTGTGTCTTCTA 42 TTCATGACTTTTTGTGCCACTGC 43 TTTTTGCCTCTACTGACC 44 TCTACACAGTCCCCTCC 45 TTTTTTTGAGTCTTCCATACCTTCT 51 TTTTGCCTTCTACACAAAAT 52 TTTTTTTTTCTGCTGCGGTTTCC 53 TTTTTCGCAACCACACAGTCTAT 56 TGTGCTGAGGATAAAAAG 58 TTTTTTTTACTGCTACAGAACAGTTAAGTAAATA 59 TGGGGCACTGAAGTAACTAAGG 66 TTTTTAAAAAACACATTAACTAA 68 TTTTTTTTTTATTGTCCACTACTACAGACTC 73 TTGTAGGTACACAGGCTAGTAG 81 TTTTTCACTGCTGTTACTCAATCTGT 82 TTTTGCTACACAGGCAAATGAATACACA 83 TGCTACATCTGCTGCT downstream degenerate primer C...

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Abstract

The invention relates to a detection method for diagnosis of human papilloma virus (HPV) infection, and utilization of a kit prepare by the method for detecting HPV genotype DNA samples isolated from the patient in order to diagnose whether it belongs to one of the 24 types, and belongs to the technical field of life science. The method of the invention comprises a polymerase chain reaction based on PCR technology; the PCR reaction comprises simultaneous detection of DNA of 24 types including HPV6, 11, 16, 18, 31, 33, 35, 39, 42, 43, 44, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 83, MM4 and cp8304 on a fluorescently-labeled upstream primers and unlabeled degenerated downstream primers of various types under particular PCR condition in a reaction tube, capillary denaturing gel electrophoresis, data read by sequenator, and division into specific types by a piece of provided software directly.

Description

Technical field: [0001] The invention relates to a detection method for diagnosing human papillomavirus (HPV) infection, and a kit made by the method to detect HPV genotypes in DNA samples isolated from patients to diagnose whether they belong to the 24 one of the types. It belongs to the field of life science and technology. Background technique: [0002] Since HPV infection is a major cause of cervical cancer, this research has now become the frontier of cervical cancer prevention. Of all malignancies in Western industrialized countries, cervical cancer is perhaps the best example of public preventive measures. [0003] Cervical cancer is the most common malignancy in women, mainly occurring in early menopause in prolific women. About 190,000 people die from cervical cancer every year, and more than three quarters of these deaths occur in developing countries. Cervical cancer ranks seventh among all cancers and third among women, after breast cancer and colorectal canc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68G01N21/64
CPCC12Q1/708C12Q1/686C12Q2600/16
Inventor 浦艳
Owner 浦艳
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