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Detection method of Wudajianjun tincture

An inspection method, the technology of general tincture, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that are not conducive to effective control of the quality of finished products, quantitative detection without drugs, etc.

Inactive Publication Date: 2015-01-21
广西壮族自治区民族医药研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The quality standard of this series of products "Wu Da Jiang Jiang Liquor" is recorded in Gui Wei Yao Pharmaceutical Zi (2003) 800005. Only the TLC identification methods for Polygonum cuspidatum, Chuanxiong, Xu Changqing, menthol, and dragon's blood are formulated, and there is no quantitative detection of drugs. It is not conducive to effectively controlling the quality of finished products

Method used

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  • Detection method of Wudajianjun tincture
  • Detection method of Wudajianjun tincture
  • Detection method of Wudajianjun tincture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 prepares martial arts general tincture

[0028] Take Polygonum cuspidatum 150g, Curcuma 100g, Xu Changqing 50g, Chuanxiong 50g, Feilongzhangxue 50g, Piper longum 50g, Drynaria fortunei 50g, Frankincense 6g, Panax notoginseng 6g, Dragon’s blood 1g, Douchi ginger 50g, Glauber’s salt 10g, Menthol 4g in total. Thirteen flavors, except for frankincense, Glauber's salt, dragon's blood, and menthol, Panax notoginseng is separately crushed into the coarsest powder (through No. Add 1800ml of 50% ethanol, airtight, and soak for 40 days; filter, and the filtrate is set aside; frankincense, mirabilite, and dragon's blood are ground separately, added to the above filtrate, stirred well, airtight, and stand for 15 days; take the supernatant, add Menthol, stir to dissolve, add 50% ethanol to a total amount of 1000ml, stir well, and get final product.

[0029] Prepare three batches of martial arts general tincture products according to the above method, and the batch numb...

Embodiment 2

[0030] TLC identification of embodiment 2 Rhizoma Chuanxiong and Curcuma

[0031] Get three batches of martial arts general tincture products of embodiment 1, carry out the TLC identification of Rhizoma Chuanxiong and Curcuma Rhizoma Rhizoma Chuanxiong by the test method that the present invention establishes, specifically as follows:

[0032] Take 30ml of this product, put it in a separatory funnel, add 30ml of water, shake well, add diethyl ether and shake to extract 2 times, 30ml each time, combine the diethyl ether, add 10g of anhydrous sodium sulfate, shake, filter, and evaporate the filtrate to dryness. Add 2ml of methanol to the residue to dissolve it as the test solution; take 1g of Rhizoma Chuanxiong reference drug powder, add 20ml of ether, heat and reflux in a water bath at 40°C for 1 hour, filter, evaporate the filtrate to dryness, add 2ml of ethyl acetate to the residue to dissolve, as a control Medicinal material solution; then take the gemmacone reference substa...

Embodiment 3

[0033] The determination of the content of polydatin and paeonol in Polygonum cuspidatum and Xu Changqing in embodiment 3

[0034] Get three batches of martial arts general tincture products of embodiment 1, carry out the content determination of polydatin in Polygonum cuspidatum and paeonol in Xu Changqing by the test method established by the present invention, specifically as follows:

[0035] Carry out with reference to liquid chromatography (Appendix VI D of Chinese Pharmacopoeia 2010 edition):

[0036] Chromatographic conditions and system suitability test: Octadecylsilane bonded silica gel is used as filler; methanol is used as mobile phase A, water is used as mobile phase B, gradient elution is carried out according to the regulations in the table below; variable wavelength detection; The flow rate is 0.8ml per minute; the number of theoretical plates should not be less than 5000 based on polydatin peak; not less than 3000 based on paeonol peak;

[0037]

[0038] P...

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Abstract

The invention discloses a detection method of Wudajianjun tincture. According to the detection method, on the basis that polygonum cuspidatum, ligusticum wallichii, paniculate swallowwort root, menthol and resina draconis in Wudajianjun liquor can be respectively identified according to the quality standard of the Wudajianjun liquor, the thin layer identification of ligusticum wallichii is improved, and the thin layer identification of curcuma zedoary is added, so that two types of medicinal materials, namely ligusticum wallichii and curcuma zedoary, can be identified at the same time under the same thin layer chromatography condition via different viewing methods by improving a silica gel G adsorbent for original standard identification to a silica gel H adsorbent and improving an N-hexane-ethyl acetate (9:1) developing solvent to petroleum ether (30-60 DEG C)-ethyl acetate (98:2); meanwhile, the measurement on the content of polygonum cuspidatum and paniculate swallowwort root is additionally carried out, i.e., the content of an effective component, polydatin, in polygonum cuspidatum and the content of an effective component, paeonol, in paniculate swallowwort root in the Wudajianjun tincture finished product are detected via high performance liquid chromatography, so that the specificity and the effectiveness of medicine quality control are improved. Thus, the quality of the Wudajianjun tincture product can be effectively controlled.

Description

technical field [0001] The invention belongs to the technical field of drug quality testing, and in particular relates to a testing method for martial arts general tincture. Background technique [0002] Wu Da Jiang Tincture is a strong medicinal preparation widely used in the treatment of bruises, tendons and bones, rheumatic bone pain, rheumatoid arthralgia, and hyperosteogeny. It is made of thirteen medicinal materials such as Feilongzhangxue, tempeh ginger, and dragon's blood. The quality standard of this series of products "Wu Da Jiang Jiang Liquor" is recorded in Gui Wei Yao Pharmaceutical Zi (2003) 800005. Only the TLC identification methods for Polygonum cuspidatum, Chuanxiong, Xu Changqing, menthol, and dragon's blood are formulated, and there is no quantitative detection of drugs. It is not conducive to effectively controlling the quality of finished products. Contents of the invention [0003] The technical problem to be solved by the present invention is to p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/02
Inventor 黄瑞松苏青陈昌源程钰琪雷沛霖陆峥琳
Owner 广西壮族自治区民族医药研究院