Plant artificial seeds having multilayers and methods for the production thereof

A technology for artificial seeds and plant tissue, applied in the fields of botany equipment and methods, plant regeneration, horticultural methods, etc., can solve problems such as limited practicability

Inactive Publication Date: 2015-01-21
EI DU PONT DE NEMOURS & CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, propagules from this method need to be hardened in a nursery before being transferred to the field, which limits their usefulness for large-scale sugarcane production

Method used

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  • Plant artificial seeds having multilayers and methods for the production thereof
  • Plant artificial seeds having multilayers and methods for the production thereof
  • Plant artificial seeds having multilayers and methods for the production thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0297] Example 1: Sugarcane Artificial Seed - Construction 1

[0298] The assembly process for making artificial sugarcane seeds was done in a non-sterile open laboratory bench environment. Cut wax paper cylinders to 3cm lengths. One opening of the wax paper cylinder was fixed through a piece of stretched gelatin-starch-glycerol film based on composition A. This fixed opening serves as the bottom opening of the cylinder. The Metromix soil was then added to the cylinder until the cylinder was approximately 1 / 3 full. Tap the cylinder on the laboratory bench to pack the soil down. 20-day-old sugarcane seedlings prepared as described above were trimmed at the root and shoot tips and placed in the Metromix. Add more Metromix to the top of the shoot again and tap again to pack the soil so that the cylinder is 3 / 4 full and the shoot tip of the shoot is visible above the soil. The Metromix was wetted with approximately 400 microliters (μl) of water, and the top opening was fixe...

example 2

[0300] Example 2: Sugarcane Artificial Seed - Construction 2

[0301] In addition to moisture from the soil outside the artificial seed, soil moisture inside the artificial seed will also cause some softening of the artificial seed structure. To prevent moisture from the Metromix inside the waxed paper cylinder from reaching the gelatin-starch-glycerol closure at the bottom opening, a second layer consisting of a paper disc was added to the gelatin-starch-glycerol closure used to secure the waxed paper before securing the opening with the gelatin-starch-glycerol film In the closure of the bottom and top openings of the cylinder ( Figure 2a with Figure 2b ). exist Figure 2a with 2b Among them, (1) is the stretched gelatin-starch-glycerol film; (2) is the wax paper cylinder; (3) is the Metromix soil; (4) is the sugarcane seedling and (5) is the gelatin-starch added to the stretching - Layers of paper discs with top and bottom openings in glycerol film.

[0302] The as...

example 3

[0306] Example 3: Sugarcane Artificial Seed - Construction 3

[0307] To eliminate moisture loss from the opening at the bottom of the wax paper, a new construction of artificial seeds was used. Wax paper cylinders were cut to 4 cm lengths and placed upright on aluminum pans placed on a 35°C hot plate. The bottom opening of the cylinder was filled with approximately 7-10 drops (or filled to a thickness of approximately 2-3 mm) of an aqueous gelatin-starch-glycerol solution (composition A). The filled cylinder was left on a hot plate for 1 hour, and then overnight at room temperature, to form a film layer at the bottom opening of the cylinder. In the next step, approximately 10 drops of molten (33-34°C) (vegetable shortening) was added to the dry gelatin-starch-glycerol film at the bottom opening of the wax paper cylinder to form a crack-free fat layer upon cooling to room temperature. The oil layer helps keep moisture within the Metromix soil in the waxed paper cylinder....

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Abstract

Composition and method for preparing artificial seeds of plantlets that can be developed into grown plants for propagation in the field are disclosed. In one embodiment, the artificial seeds are developed in degradable containers. The disclosed methods also allow for rapid propagation of in demand plants, such as sugarcane, to meet the ever increasing global demand for this plant.

Description

[0001] Cross References to Related Applications [0002] The benefit of US Provisional Application 61 / 578,432, filed December 21, 2011, is claimed under 35 U.S.C. § 119(e), the disclosure of which is incorporated herein by reference in its entirety. technical field [0003] The present invention relates to the preparation of plant artificial seeds. In particular, it relates to the preparation of sugarcane artificial seeds. Background technique [0004] Some plants such as sugar cane, bananas, pineapples, citrus, conifers and apples cannot be propagated by seeds due to: a) the loss of genetic identity during seed propagation; b) the long period of growth required for the plants before producing seeds; and c ) The natural seeds of these plants grow and survive poorly in field growing conditions. These crops are currently propagated by vegetative methods or via seedling propagation. Attempts have therefore been made to develop various economical alternatives to their propag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G9/02A01H4/00
CPCA01H4/006A01G9/1026A01G1/001A01G9/0293A01G22/55
Inventor T.卡斯帕D.加斯帕雷托L.D.高特内伊R.吉莫尔B.哈拉汉D.L.哈拉汉B.D.约翰逊B.H.乔内斯K.克拉茨P.拉克斯马南S.马哈詹B.D.马瑟B.A.莫里斯M.L.农赫兹J.A.佩罗托J.许
Owner EI DU PONT DE NEMOURS & CO
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