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Aquaculture rapid propagation method for potato virus-free tissue culture plantlets

A technology of potato and tissue culture seedlings, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of high production cost of original seeds, high production environment requirements, and high cost of culture solution, and meet the production environment requirements. High efficiency, low cost, and high transplanting survival rate

Active Publication Date: 2015-01-28
LIJIANG BOFU AGRI TECH DEV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, there are mainly two ways to propagate virus-free potato plantlets in test tubes. One is that potato-free test-tube plantlets are directly propagated in vermiculite, perlite, sand, sawdust, mountain soil and other substrates. Low rate, easy to infect germs, resulting in high production cost of the original seed; the other is the propagation of potato virus-free test tube seedlings in MS-based culture solution, this method has a faster propagation speed and a higher survival rate, but the production High environmental requirements and high cost of culture medium also lead to high production cost of original species

Method used

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Examples

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Embodiment Construction

[0009] Below in conjunction with embodiment the present invention is described in detail.

[0010] 1. Cut the stem tips of the virus-free test-tube seedlings of potatoes and insert them into the holes of the foam board. The row spacing of the cuttings is 3cm×4cm. 1.5cm to 2cm, cultured for 5 to 7 days at a temperature of 22°C to 26°C and a humidity of 70% to 75%, to complete rooting. The formula of the rooting nutrient solution is:

[0011] KNO 3 100~120㎎∕L

[0012] NH 4 NO 3 780~900㎎∕L

[0013] MgSO 4 65~80㎎∕L

[0014] CaCl 2 2H 2 O 70~90㎎∕L

[0015] K H 2 PO 4 30~40㎎∕L

[0016] Na 2 -EDTA 1.1~1.3ɡ∕L

[0017] FeSO 4 ·7H 2 O 0.91~0.99ɡ∕L

[0018] The rooting nutrient solution is prepared with sterile water.

[0019] 2. Replace the rooting nutrient solution in the hydroponic tank with the strong seedling nutrient solution. The depth of the strong seedling nutrient solution is 2cm to 3cm. Let the seedlings in step 1 float in the ...

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Abstract

The invention provides an aquaculture rapid propagation method for potato virus-free tissue culture plantlets. The aquaculture rapid propagation method is characterized by comprising the following steps: 1, shearing stem tip sections off potato virus-free test-tube plantlets and inserting in holes of a foam plate, putting in an aquaculture plantlet tray, standing and floating in a rooting nutrient solution, culturing for five to seven days, and finishing rooting; 2, standing and floating the plantlets in step 1 in a plantlet strengthening nutrient solution to culture for 12-15 days, and using the plantlets growing four to five leaves as parent seedlings; 3, shearing stem tips off the parent seedlings cultured in step 2, carrying out cuttage by step 1 and step 2, rooting, culturing, using as parent seedlings again after the seedlings grow four to five leaves, shearing off stem tips, carrying out cuttage by step 1 and step 2, rooting and culturing; 4, shearing stem tips off the parent seedlings in step 3 for six to eight times, transplanting as seedlings, and producing breeder's seeds. When the method is used for propagating the potato virus-free test-tube plantlets, the production cost is low, and the quality is good.

Description

technical field [0001] The invention relates to a propagation method of virus-free potato tissue-cultured seedlings, in particular to a hydroponic rapid propagation method of virus-free potato tissue-cultured seedlings. Background technique [0002] At present, there are mainly two ways to propagate virus-free potato plantlets in test tubes. One is that potato-free test-tube plantlets are directly propagated in vermiculite, perlite, sand, sawdust, mountain soil and other substrates. Low rate, easy to infect germs, resulting in high production cost of the original seed; the other is the propagation of potato virus-free test tube seedlings in MS-based culture solution, this method has a faster propagation speed and a higher survival rate, but the production The high environmental requirements and the high cost of the culture medium also lead to high production costs of the original species. Contents of the invention [0003] The purpose of the present invention is aimed at ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 宋伯符杨铁成宋文芳
Owner LIJIANG BOFU AGRI TECH DEV
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