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Separation and purification method for sulfate reducing bacteria in sewage of oil field

An oilfield sewage, separation and purification technology, applied in the field of petroleum microorganisms, can solve the problems of high operator requirements, complex operation, time-consuming and other problems, and achieve the effects of low operator requirements, simple operation and high success rate.

Inactive Publication Date: 2015-01-28
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing SRB separation and purification method has following three kinds: dilute shaking tube method, stacked dish interlayer method and Hungate rolling tube method, but present method has the following problems: (1) dilute shaking tube method is more difficult to observe and pick bacterial colony , time-consuming, laborious and inefficient; (2) the scope of application of the stacked dish sandwich method is small, and the success rate of separation and purification is low, and it often takes 3 to 6 repetitions of separation and purification to obtain a relatively purified culture; (3) the Hungate rolling tube is The requirements for equipment operators are high, the operation is complicated, and the separation and purification effect is low; (4) The separation time of the three methods all needs more than 10d

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  • Separation and purification method for sulfate reducing bacteria in sewage of oil field
  • Separation and purification method for sulfate reducing bacteria in sewage of oil field
  • Separation and purification method for sulfate reducing bacteria in sewage of oil field

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Embodiment 1

[0034] The method of the present invention is used to separate and purify SRB in the produced water of a block A in Shengli Oilfield. The temperature of the produced water in block A is 30°C. The specific steps are as follows:

[0035] 1. Preparation of SRB enriched medium

[0036] The enrichment medium was dissolved in distilled water, anaerobically distributed into anaerobic tubes with a volume of 25ml, 9ml per tube, and sterilized by high-pressure steam at 121°C for 30min.

[0037] Among them, the enrichment medium formula is 0.4% of sodium lactate, 0.05% of ferrous ammonium sulfate, 0.07% of yeast extract powder, 1.0% of sodium chloride and 0.03% of magnesium chloride.

[0038] 2. Preparation of SRB isolation medium

[0039] Dissolve the separation medium in distilled water, heat until it is fully dissolved, then anaerobically distribute it into anaerobic tubes with a volume of 25ml, 15ml per tube, sterilize with high-pressure steam at 121°C for 20min, and cool down to 50...

Embodiment 2

[0048] The method of the present invention is used to separate and purify SRB in the produced water of a block B in Shengli Oilfield. The temperature of the produced water in block B is 48°C. The specific steps are as follows:

[0049] 1. Preparation of SRB enriched medium

[0050] The enriched medium was dissolved in distilled water, anaerobically dispensed into anaerobic tubes with a volume of 25ml, each tube was dispensed with 9ml, and sterilized by high-pressure steam at 121°C for 20min.

[0051] Among them, the enrichment medium formula is 0.5% of sodium lactate, 0.2% of ferrous ammonium sulfate, 0.1% of yeast extract powder, 0.5% of sodium chloride and 0.01% of magnesium chloride.

[0052] 2. Preparation of SRB isolation medium

[0053] Dissolve the separation medium in distilled water, heat until it is fully dissolved, then anaerobically distribute it into anaerobic tubes with a volume of 25ml, each tube is 15ml, sterilize by high-pressure steam at 121°C for 25min, and...

Embodiment 3

[0062] The method of the present invention is used to separate and purify SRB in the produced water of a block C in Shengli Oilfield. The temperature of the produced water in block C is 60°C. The specific steps are as follows:

[0063] 1. Preparation of SRB enriched medium

[0064] The enrichment medium was dissolved in distilled water, anaerobically dispensed into 25ml anaerobic tubes, 9ml per tube, and sterilized by high-pressure steam at 121°C for 25min.

[0065] Among them, the enrichment medium formula is 0.6% of sodium lactate, 0.1% of ferrous ammonium sulfate, 0.05% of yeast extract powder, 0.08% of sodium chloride and 0.02% of magnesium chloride.

[0066] 2. Preparation of SRB isolation medium

[0067] Dissolve the separation medium in distilled water, heat until it is fully dissolved, then anaerobically distribute it into anaerobic tubes with a volume of 25ml, 15ml per tube, sterilize with high-pressure steam at 121°C for 30min, and cool down naturally to 55°C after ...

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Abstract

The invention discloses a separation and purification method for sulfate reducing bacteria in sewage of an oil field. The separation and purification method comprises the following specific steps of: (1) preparation of an SRB enriching medium; (2) preparation of an SRB separation medium; (3) enriching culture of the SRB; (4) separated culture of the SRB; and (5) purified culture of the SRB. The separation and purification method disclosed by the invention has the advantages that the speed for separation and purification is high, the process from preparation of the SRB enriching medium and the SRB separation medium to purification of a cultured matter only needs 3-5 days, the success rate of separation and purification is high, the purified cultured matter can be obtained by one-time separation and purification, the operation is simple, the requirement for operators is low, and the targeting performance is strong. Due to only suitability for separation and purification of the SRB, the separation and purification method can be widely applied in separation and purification of the sulfate reducing bacteria in the sewage of the oil field.

Description

1. Technical field [0001] The invention belongs to the technical field of petroleum microorganisms, in particular to a method for separating and purifying sulfate-reducing bacteria in oilfield sewage. 2. Background technology [0002] Sulfate-reducing bacteria (SRB) are microorganisms that use sulfate or other oxidized sulfides in oilfield production fluids as electron acceptors to dissimilate organic substances. 4 2- reduced to H 2 S, which in turn changes the pH value of the oilfield water quality and the corrosion state of the metal, is an important factor for the significant increase in the metal corrosion rate, and brings many harmful effects such as corrosion and blockage to the oilfield pipeline network. [0003] Separation and purification of SRB to obtain a pure SRB single strain can provide an important theoretical basis for the analysis of the colony structure of the oilfield environment and the evaluation of the corrosion status and water quality. [0004] Exi...

Claims

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Application Information

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IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 潘永强徐鹏袁长忠王刚徐闯李彩风杜春安王静宋欣张守献曹嫣镔
Owner CHINA PETROLEUM & CHEM CORP