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Molecular marker suitable for detecting radish leaf margin lobe trait and application of molecular marker

A technology of molecular markers and radish leaves, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, DNA preparation, etc., to reduce the breeding workload, speed up the process, and shorten the breeding period

Active Publication Date: 2015-01-28
WUHAN SHUBO AGRI TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the molecular markers of radish leaf margin cracking, and there is no report on the breeding selection of radish leaf margin cracking using molecular marker-assisted selection.

Method used

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  • Molecular marker suitable for detecting radish leaf margin lobe trait and application of molecular marker
  • Molecular marker suitable for detecting radish leaf margin lobe trait and application of molecular marker
  • Molecular marker suitable for detecting radish leaf margin lobe trait and application of molecular marker

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Experimental program
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Embodiment 1

[0038] 1. Construct the radish leaf edge crack separation population:

[0039] In this example, F 1 , the F 1 Bagging self and backcross respectively, resulting in F 2 and BC 1 .

[0040] 2. Phenotypic identification of leaf margin cleavage:

[0041] Phenotyping radish leaf shape in the field, investigating F 2 Groups and BC 1 The leaf shape segregation ratio of each individual plant in the population was analyzed by chi-square test (x 2) Inheritance law of leaf margin cleavage. The test method refers to the editor-in-chief of Nanjing Agricultural University, Field Experiments and Statistical Methods, China Agricultural Press, 1985, second edition. The result is, (radish "Wuqing No. 1" x radish "Xinzhou Huaye") F 1 BC produced by backcrossing with parent "Wuqing No. 1" 1 In the population, 42 plants had lobed leaves, 39 plants had fully lobed leaves, x 2 Check that the segregation ratio conforms to 1:1, and the BC produced by backcrossing with the parent "Xinzhou Hu...

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Abstract

The invention belongs to the technical field of preparation of molecular markers of vegetables and particularly relates to a molecular marker suitable for detecting the radish leaf margin lobe trait and an application of the molecular marker. A molecular marker HY-18 which is tightly interlocked with a radish leaf margin lobe gene is obtained through the steps of selecting an appropriate parent to hybridize to obtain a plant F1; seeding a seed F1, bagging the plant F1 to inbreed and backcross to obtain segregation populations F2 and BC1; constructing a radish leaf supersulcus gene pool and a total leaf lobe gene pool, designing a specific primer, carrying out PCR, recovering, cloning, sequencing and screening; and the nucleotide sequence of the molecular marker is as shown in SEQ ID NO.1 in a sequence table. The invention also discloses a preparation method of the molecular marker. The molecular marker prepared by using the preparation method can be used for analyzing radish leaf lobe populations, and the practical marker and method are provided for molecular breeding of radishes.

Description

technical field [0001] The invention belongs to the technical field of preparation of vegetable molecular markers, and in particular relates to a molecular marker suitable for detecting cracked properties of radish leaf margins and its application. The molecular markers can be used as markers for the breeding of radish leaf edge cracking traits, and provide molecular markers for cultivating genetically stable new varieties of radish with leaf edge cracking. Background technique [0002] Leaf is the main organ of plants for photosynthesis, respiration and transpiration, which affects the photosynthetic efficiency, yield and quality of plants. The shape of plant leaves is composed of several parts such as leaf shape, leaf tip, leaf base and leaf edge, among which the leaf edge has shapes such as whole edge, sawtooth, crack, and wave. Moderate cracking of the leaf edge can change the plant type, improve the ventilation of the colony, the light transmittance and the light energ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C12N15/10
CPCC12Q1/6806C12Q1/6895C12Q2600/13C12Q2600/158
Inventor 熊秋芳张雪丽张小康张雪清徐长城
Owner WUHAN SHUBO AGRI TECH CO LTD
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