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Preparation method and application of a tio2-cdse nanocomposite photoelectric biosensor

A nanocomposite material and biosensor technology, which is applied in the analysis of materials, material analysis by optical means, material analysis by electromagnetic means, etc., can solve problems such as low application value, achieve small quantum size effect, and expand the scope of use. , Enhance the effect of photoelectric performance

Inactive Publication Date: 2016-03-02
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Looking at the development of aptasensors, it can be found that most research groups focus on short-strand DNA (20-60bp) for DNA sensors, and most of these DNAs are designed according to certain rules in advance according to needs, and then by Synthesized by biological companies, but the DNA fragment does not actually exist, and the application value is not great

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 A kind of TiO 2 -Preparation method of CdSe nanocomposite photoelectric biosensor

[0034] (1) Clean a 1.0cm×2.5cm rectangular ITO conductive glass with acetone, ultrapure water, and ethanol for 30 minutes, and dry it with pure nitrogen. Use it as the working electrode, platinum wire as the counter electrode, and the reference electrode as saturated glycerin. Mercury electrode, with a scanning speed of 0.05~0.2V / s, in the potential range of -1.5~2.5V, in the presence of 5×10 -3 mol / L L-lysine, PBS with a pH of 6.0, use cyclic voltammetry to electropolymerize L-lysine on the surface of the working electrode to form a layer of poly-L-lysine, and store in a vacuum desiccator ;

[0035] (2) Drop-coat 2 μL of TiO 2 - Solid CdSe complex or 2 μL of TiO 2 - Put the liquid CdSe complex on (1) the surface of the modified working electrode, and dry it in a refrigerator at 4°C;

[0036] (3) Continue to drop-spray 10 μL, 5 μg / mL upstream primer of target DNA or 10 μ...

Embodiment 2

[0038] Example 2 A kind of TiO 2 -Preparation method of CdSe nanocomposite photoelectric biosensor

[0039] (1) Clean a 1.0cm×2.5cm rectangular ITO conductive glass with acetone, ultrapure water, and ethanol for 30 minutes, and dry it with pure nitrogen. Use it as the working electrode, platinum wire as the counter electrode, and the reference electrode as saturated glycerin. Mercury electrode, with a scanning speed of 0.05~0.2V / s, in the potential range of -1.5~2.5V, in the presence of 5×10 -3In mol / L L-lysine and PBS with a pH of 7.4, use cyclic voltammetry to electropolymerize L-lysine on the surface of the working electrode to form a layer of poly-L-lysine, and store it in a vacuum desiccator ;

[0040] (2) Drop-coat 10 μL of TiO 2 - Solid CdSe composite or 10 μL of TiO 2 - Put the liquid CdSe complex on (1) the surface of the modified working electrode, and dry it in a refrigerator at 4°C;

[0041] (3) Continue to drip-coat 15 μL, 25 μg / mL upstream primer of target...

Embodiment 3

[0043] Example 3 A kind of TiO 2 -Preparation method of CdSe nanocomposite photoelectric biosensor

[0044] (1) Clean a 1.0cm×2.5cm rectangular ITO conductive glass with acetone, ultrapure water, and ethanol for 30 minutes, and dry it with pure nitrogen. Use it as the working electrode, platinum wire as the counter electrode, and the reference electrode as saturated glycerin. Mercury electrode, with a scanning speed of 0.05~0.2V / s, in the potential range of -1.5~2.5V, in the presence of 5×10 -3 mol / L L-lysine, PBS with a pH of 9.0, use cyclic voltammetry to electropolymerize L-lysine on the surface of the working electrode to form a layer of poly-L-lysine, and store in a vacuum desiccator ;

[0045] (2) Drop-coat 18 μL of TiO 2 - Solid CdSe composite or 18 μL of TiO 2 - Put the liquid CdSe complex on (1) the surface of the modified working electrode, and dry it in a refrigerator at 4°C;

[0046] (3) Continue to drip-coat 20 μL, 50 μg / mL upstream primer of target DNA or ...

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PUM

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Abstract

The invention relates to a preparation method and application of a TiO2-CdSe nano-composite photoelectric biosensor and belongs to the technical field of biosensing detection. Based on the TiO2-CdSe composite as the photoelectric beacon material, CdSe quantum dots enhance the photoelectric performance of TiO2 with its quantum size effect, dielectric effect and surface area effect, and can realize the sensitivity and specificity of target DNA and its housekeeping genes in solid tissues. and rapid detection, the sensor prepared by the invention has the advantages of simple equipment, low cost, easy miniaturization and integration, and is easy to popularize.

Description

technical field [0001] The present invention relates to a kind of TiO 2 -CdSe nanocomposite photoelectric biosensor preparation method and its application, the sensor is used for the detection of biological tissue DNA, which belongs to the technical field of biosensing detection. Background technique [0002] Looking at the development of aptamer sensors, it can be found that the research of most research groups on DNA sensors mainly focuses on short-chain DNA (20-60bp), and most of these DNAs are designed according to certain rules in advance according to needs, and then by The biological company synthesizes it by contract, but the DNA fragment does not actually exist, and its application value is not great. If DNA biosensors are to be used for actual detection, the detected DNA should be real. This requires finding the DNA that needs to be detected and identified from practical problems, and then designing sensors according to needs and applying them in practice. [0003...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/00G01N27/416
Inventor 庞雪辉闫涛魏琴朱宝存范大伟马洪敏
Owner UNIV OF JINAN
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