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Heterologous expression and application of thermal-stability carbonic anhydrase

A carbonic anhydrase and thermostability technology, applied in the field of biological enzymes, can solve problems such as the construction of pilot-scale demonstration devices and industrial application, etc.

Inactive Publication Date: 2015-02-04
LIVINGZONE SHANGHAI BIO CHEM TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, using carbonic anhydrase for CO 2 Capture is still in the research and development stage, and there are no reports on the construction of pilot demonstration devices and industrial applications

Method used

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  • Heterologous expression and application of thermal-stability carbonic anhydrase
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  • Heterologous expression and application of thermal-stability carbonic anhydrase

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Embodiment Construction

[0027] The technical solution of the present invention is described in detail below through the examples, and the following examples are only exemplary and can only be used to explain and illustrate the technical solution of the present invention, rather than being interpreted as a limitation to the technical solution of the present invention.

[0028] The present invention provides a heterologous expression of thermostable carbonic anhydrase, said thermostable carbonic anhydrase is,

[0029] A polypeptide having an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO:2. More preferably at least 98% identity, most preferably 100% identity.

[0030] A gene encoding the polypeptide of claim 1.

[0031] Its nucleotide sequence is shown in SEQ ID NO:1.

[0032] The nucleotide sequence coding of carbonic anhydrase realizes heterologous soluble expression in Escherichia coli.

[0033] The carbonic anhydrase maintains activity at pH 6.0-9.0. ...

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Abstract

The invention relates to heterologous expression and an application of a thermal-stability carbonic anhydrase, and the application of the carbonic anhydrase in the CO2 absorption field; the carbonic anhydrase has good organic amine tolerance, and can promote the absorption of organic amine on CO2. The invention also relates to the isolated amino acid coded thermal-stability carbonic anhydrase, and a nucleotide nucleic acid construction and expression way.

Description

technical field [0001] The invention belongs to the field of biological enzymes, and specifically refers to the heterologous expression and application of a heat-stable carbonic anhydrase. Background technique [0002] CO 2 The emission reduction of greenhouse gases such as GHG has attracted more and more attention from the international community, and has become a hot spot in the research and development of the international energy field. The world emits more than 30 billion tons of CO every year 2 , resulting in a severe greenhouse effect. Therefore, CO 2 The capture and storage (Carbon Capture and Storage, referred to as CCS) has become urgent. [0003] There are currently three main different types of CO 2 Capture systems, namely pre-combustion decarbonization (Pre-combustion), oxy-fuel combustion (Oxy-fuel combustion) and post-combustion decarbonization (Post-combustion), the most mature of which is post-combustion decarbonization, that is, after combustion Captur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/88C12N15/60C12N15/70B01D53/84B01D53/62
CPCB01D53/62B01D53/84C12N9/88C12Y402/01001Y02P20/59Y02A50/20
Inventor 祝俊余允东龙辉周晓青张燕张敏汪浩陈婷婷
Owner LIVINGZONE SHANGHAI BIO CHEM TECH CO LTD