Production method of disporum cantoniense tissue culture seedlings

A production method and technology of tissue culture seedlings, applied in the production of Wanshou bamboo tissue culture seedlings, and in the field of artificially cultivated Wanshou bamboo seedlings, to achieve the effects of uniform properties, fast propagation speed, and improved multiplication ability

Inactive Publication Date: 2015-02-18
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of the rapid propagation of Wanshou bamboo, to provide a method for fast and mass production of higher quality Wanshou bamboo tissue culture seedlings, to overcome the deficiencies in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: a kind of method for tissue culture and rapid propagation of Longevity bamboo, the method comprises the steps:

[0015] 1) Prepare medium: cluster bud induction medium is MS + 6-BA 1.2 mg·L -1 + KT 0.45 mg·L -1 + sucrose 20g·L -1 , the rooting medium is 1 / 2 MS + NAA 0.3 mg·L -1 , all media were supplemented with agar 6g / L, and the pH was 5.8~6.0. .

[0016] 2) Treatment of inoculation materials: Select healthy sterile seedlings, remove terminal buds on an aseptic operating table, and cut them into stem segments with 1 node, the length of the stem segment below the node is 3 mm.

[0017] 3) Inoculation: Inoculate the cut stem segments on the corresponding medium at a density of 12 plants per bottle to induce clustered buds or rooting culture.

[0018] 4) Cultivation: After inoculation, place in a culture room with a culture temperature of 23°C, a light of 1000 lx, and a light time of 10 h / d for culture. Pay attention to the indoor disinfection of the...

Embodiment 2

[0020] Embodiment 2: a kind of method for tissue culture and rapid propagation of Longevity bamboo, the method comprises the steps:

[0021] 1) Prepare medium: cluster bud induction medium is MS + 6-BA 1.5 mg·L -1 + KT 0.4 mg·L -1 + sucrose 18g·L -1 , the rooting medium is 1 / 2 MS + NAA 0.5 mg·L -1 , all media were supplemented with agar 6g / L, and the pH was 5.8~6.0. .

[0022] 2) Treatment of inoculation materials: Select healthy sterile seedlings, remove terminal buds on an aseptic operating table, and cut them into stem segments with 1 node, the length of the stem segment below the node is 5 mm.

[0023] 3) Inoculation: Inoculate the cut stem segments on the corresponding medium at a density of 15 plants per bottle to induce clustered buds or rooting culture.

[0024] 4) Cultivation: After inoculation, place in a culture room with a culture temperature of 25°C, a light of 1500 lx, and a light time of 12 h / d for culture. Pay attention to the indoor disinfection of the ...

Embodiment 3

[0026] Embodiment 3: a kind of method for tissue culture and rapid propagation of Longevity bamboo, the method comprises the steps:

[0027] 1) Prepare medium: cluster bud induction medium is MS + 6-BA 1.5 mg·L -1 + KT 0.35 mg·L -1 + sucrose 30g·L -1 , the rooting medium is 1 / 2 MS + NAA 0.2 mg·L -1 , all media were supplemented with agar 7g / L, and the pH was 5.8~6.0. .

[0028] 2) Treatment of inoculated materials: select healthy sterile seedlings, remove terminal buds on an aseptic operating table, and cut them into stem segments with 1 node, the length of the stem segment below the node is 8 mm.

[0029] 3) Inoculation: Inoculate the cut stem segments on the corresponding medium at a density of 18 plants per bottle to induce clustered buds or rooting culture.

[0030] 4) Cultivation: After inoculation, place in a culture room with a culture temperature of 27°C, a light of 2000 lx, and a light time of 15 h / d for culture. Pay attention to the indoor disinfection of the ...

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Abstract

The invention discloses a production method of disporum cantoniense tissue culture seedlings. The method mainly comprises the following steps: by taking stem segments respectively having one node as tissue culture materials, carrying out tissue culture seedling production on a cluster bud inducing medium and a rooting medium, wherein the cluster bud inducing medium and the rooting medium are prepared before inoculating, treating the inoculated stem segments and then carrying out inoculated culture on the stem segments according to the density of 15 stem segments in each bottle. The method is simple to operate and high in propagation speed, so that the propagation quantity is greatly increased, the propagation coefficient of cluster buds can reach 6.79 and the rooting rate of the cluster buds can reach 68.89% after the cluster buds are cultured for about 80 days. In addition, the stem segments respectively having one node are taken as the tissue culture materials, so that the propagation speed is high and the propagation quantity is large; and the content of saccharose in the cluster bud inducing medium is 20g / L, so that the production cost is lowered and the economic benefit is increased.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for producing Wanshou bamboo tissue cultured seedlings, which is a seedling raising method mainly aimed at the artificial cultivation of local Wanshou bamboos in Guizhou. Background technique [0002] Disporum cantoniense ( Lour. ) Merr. is a perennial herbaceous plant of the genus Disporum in the family Liliaceae. Its roots and rhizomes are used as medicine and are called ginseng. Hundred-tailed ginseng is also known as white-flavored ginseng, hundred-tailed bamboo shoots, and white dragon beard. The peasants in the place where the medicinal materials are produced call them bamboo umbrellas, rice umbrellas, oxtail ginseng, and bamboo-jointed ginseng. It is sweet, light and flat in nature. It has the effects of nourishing the lungs and relieving cough, invigorating the spleen and eliminating stagnation. Baiwei ginseng is one of the main raw materials in the "k...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 索贝贝王华磊赵致朱力顾岑
Owner GUIZHOU UNIV
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