Multifunctional TA cloning vector system for blue-white selection and application of multifunctional TA cloning vector system

A cloning vector and blue-white screening technology, applied in the field of molecular biology, can solve the problems of high false positive and false negative rates, reduce false positives and false negatives, and save costs and time
CN104357472AInactive Publication Date: 2015-02-18SUZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUZHOU UNIV
Publication Date
2015-02-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a multifunctional TA cloning vector system by a method of regulating a coding framework. Compared with a conventional commercialized TA vector, the multifunctional TA cloning vector has the advantages that the false positive and false negative rate during TA cloning can be reduced, and the self-ligation phenomenon of the vector is remarkably improved. The multifunctional TA cloning vector system has the creative application of providing two selections of turning white from blue and turning blue from white to a bacterial colony after the TA cloning vector is inserted into a sequence, wherein the false positive and false negative rates are both lower than 1 percent in the section of turning blue from white. Moreover, the vector can be used for detecting minimal lesion of frameshift mutation of tumor related genes, and is a unique product with the detecting function on current market, and can be used for detecting effectiveness of trans factor protease TALENs.
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Description

technical field

[0001] The invention belongs to the field of molecular biology and relates to medical diagnosis and biotechnology. By adjusting the coding frame method, three TA cloning vectors were constructed. Compared with conventional commercial TA vectors, in addition to retaining the screening from blue to white, a new selection from white to blue is added, which can be used for the detection of small mutations and the detection of anti-factor nuclease activity. Background technique

[0002] At present, most of the cloning vectors in molecular biology experiments are artificially constructed, basically with selectable genetic markers or phenotypic characteristics. Blue-white screening (β-galactosidase system) is a more commonly used selection marker.

[0003] Such vectors carry the lacZ' gene, which encodes the N-terminus (α-peptide) of β-galactosidase, and in the inducer IPTG (isopropyl-β-D-thiogalactoside, structurally similar to lactose but cannot be degraded by ...

Claims

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