GP73 detection kit

A detection kit, -73GP73 technology, applied in the Golgi Glycoprotein-73 detection kit and its preparation field, can solve the problems of accuracy, sensitivity, poor repeatability, unsuitable for clinical use, cumbersome operation, etc., to achieve hemolysis Small interference, high specificity, quick and easy operation

Active Publication Date: 2015-02-18
ZYBIO INC
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Currently known Golgi Glycoprotein-73 (GP73) detection methods still have many defects, such as cumbersome operation, time-consuming, not suitable for clinical use
At present, the accuracy, sensitivity and repeatability of Golgi Glycoprotein-73 (GP73) detection kits on the Chinese market are still poor

Method used

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Embodiment 1

[0044] Preparation of test paper card of the present invention:

[0045] 1) Use the pretreatment buffer to pretreat the gold standard pad. The pretreatment buffer is: stachyose 2g / L, alum 0.5g / L, fructose sodium diphosphate 1.5g / L, sodium hexametaphosphate 0.3g / L L, glycine 1.88g / L aqueous solution, pH = 7.4, the specific steps of pretreatment are: soak the gold standard pad in the pretreatment solution for 2 hours, take it out and dry it at 37°C; then use fluorescent microspheres to mark the Golgi apparatus The glycoprotein-73GP73 antibody solution was sprayed on the pretreated gold label pad to prepare the gold label pad coated with the Golgi glycoprotein-73GP73 antibody. The mass ratio of the fluorescent microspheres to the antibody in the solution was 5:1, and the concentration of the solution was 10mg / ml, the spray volume is 4ul / cm;

[0046] 2) Spray 1 mg / ml of Golgi glycoprotein-73GP73 antibody solution and goat anti-mouse antibody solution on the detection line and qua...

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Abstract

The invention relates to the field of biological detection and particularly relates to a detection kit for quantitatively detecting golgi apparatus glycoprotein-73GP73 as well as a preparation method and application thereof. The detection kit for quantitatively detecting the golgi apparatus glycoprotein-73GP73, provided by the invention, comprises a test paper card, wherein the test paper card comprises a bottom plate, and a sample pad, a gold marker pad, a nitrocellulose membrane and a water suction pad which are located on the surface of the bottom plate and are sequentially arrayed from a feeding end; the gold marker pad contains a golgi apparatus glycoprotein-73GP73 antibody; the nitrocellulose membrane is coated with a detection line and a quality control line; and the golgi apparatus glycoprotein-73GP73 antibody on the gold marker pad is marked by fluorescent microspheres. According to the detection kit provided by the invention, the golgi apparatus glycoprotein-73GP73 is detected by a fluorescent microsphere immunochromatography technology for the first time, and the detection kit has sensitivity and specificity; and the detection kit has the advantages of rapidness and convenience for operation, accurate result, affordable property and the like.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a Golgi apparatus glycoprotein-73 detection kit and its preparation method and application. Background technique [0002] Golgi glycoprotein-73 (GolgiProtein 73, GP73), also known as type II Golgi membrane protein (Golgi phosphoprotein2, GOLPH 2), its concentration in the serum of patients with chronic hepatitis and cirrhosis increases, while in the serum of patients with hepatocellular carcinoma The increase is more obvious, and there is a significant difference between normal people or patients with benign liver diseases. Studies have shown that serum GP73 is better than serum GP73 in the diagnosis of liver cancer. According to literature reports, the sensitivity of GP73 to detect liver cancer is 69%, and the specificity is 75%. For the diagnosis of early liver cancer, the sensitivity of GP73 was 62%, while the sensitivity of GP73 was only 25%. Among the liver cancer pati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/533
Inventor 吉权
Owner ZYBIO INC
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