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Preparation method and application of nano diamond targeted medicine

A nano-diamond and drug technology, applied in the fields of nano-drugs, nano-technology, nano-technology, etc., can solve rare problems and achieve the effect of stable chemical properties, good biocompatibility and high biocompatibility

Inactive Publication Date: 2015-03-04
SHANXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the preparation of targeting carriers is important, but whether the targeting carrier materials can truly realize the targeted delivery of drugs is the focus of people's urgent attention. As far as we know, such reports are rare at present

Method used

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  • Preparation method and application of nano diamond targeted medicine
  • Preparation method and application of nano diamond targeted medicine
  • Preparation method and application of nano diamond targeted medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Preparation of transferrin-doxorubicin (Tf-DOX) complex

[0022] Accurately weigh 2 mg of Tf, add 2 mL of PBS (pH7.4) buffer solution, shake well, then add 200 μg of DOX to it, and react overnight on a shaker at room temperature to prepare the Tf-DOX complex. React overnight on a homogenizer to prepare Tf-DOX complexes.

[0023] (2) Preparation of nanodiamond-(transferrin-doxorubicin) nanomedicine

[0024] Weigh 2mg of dry carboxylated nanodiamonds, then add 2.0ml of MES (0.1M, pH6.00) buffer solution, ultrasonically disperse for 30min to form a suspension, then add 0.4mg of EDC and 0.6mg of NHS, and stir at room temperature for 6h , centrifuged at 15000rpm for 5min, removed the supernatant, continued to wash twice with pH 7.4 PBS buffer, discarded the supernatant, and obtained the nano-diamond precipitate of the activated carboxyl group; redispersed the nano-diamond precipitate of the activated carboxyl group into the PBS buffer In the liquid solution, ultrasonic...

Embodiment 2

[0026] (1) Preparation of transferrin-doxorubicin (Tf-DOX) complex

[0027] Accurately weigh 2 mg of Tf, add 2 mL of PBS (pH7.4) buffer solution, shake well, then add 160 μg of DOX to it, and react overnight on a shaker at room temperature to prepare the Tf-DOX complex. React overnight on a homogenizer to prepare Tf-DOX complexes.

[0028] (2) Preparation of nanodiamond-(transferrin-doxorubicin) nanomedicine

[0029] Weigh 2mg of dry carboxylated nanodiamonds, then add 2.0ml of MES (0.1M, pH6.00) buffer solution, ultrasonically disperse for 30min to form a suspension, then add 0.4mg of EDC and 0.6mg of NHS, and stir at room temperature for 6h , centrifuged at 15000rpm for 5min, removed the supernatant, continued to wash twice with pH 7.4 PBS buffer, discarded the supernatant, and obtained the nano-diamond precipitate of the activated carboxyl group; redispersed the nano-diamond precipitate of the activated carboxyl group into the PBS buffer In the liquid solution, ultrasonic...

Embodiment 3

[0031] (1) Preparation of transferrin-doxorubicin (Tf-DOX) complex

[0032] Accurately weigh 2 mg of Tf, add 1 mL of PBS (pH7.4) buffer solution, shake well, then add 120 μg of DOX to it, and react overnight on a shaker at room temperature to prepare Tf-DOX complex. React overnight on a homogenizer to prepare Tf-DOX complexes.

[0033] (2) Preparation of nanodiamond-(transferrin-doxorubicin) nanomedicine

[0034]Weigh 2mg of dry carboxylated nanodiamonds, then add 2.0ml of MES (0.1M, pH6.00) buffer solution, ultrasonically disperse for 30min to form a suspension, then add 0.4mg of EDC and 0.6mg of NHS, and stir at room temperature for 6h , centrifuged at 15000rpm for 5min, removed the supernatant, continued to wash twice with pH 7.4 PBS buffer, discarded the supernatant, and obtained the nano-diamond precipitate of the activated carboxyl group; redispersed the nano-diamond precipitate of the activated carboxyl group into the PBS buffer In the liquid solution, ultrasonically ...

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Abstract

The invention provides a preparation method and application of a Tf (transferrin) modified nano diamond targeted medicine. The preparation method of the nano diamond targeted medicine comprises the following steps: dissolving transferrin Tf into a PBS (phosphate buffer solution), then adding DOX (doxorubicin) and reacting to obtain a Tf-DOX compound; and carrying out reaction on carboxylated nano diamond and the Tf-DOX compound to obtain the nano diamond targeted medicine ND-(Tf-DOX). The nano diamond targeted medicine acts on HepG2, and treatment on cells by virtue of low temperature, different inhibitors and free Tf shows that the nano diamond targeted medicine has energy dependence; with normal human HEK293 as reference, study on absorption of the cells for the nano diamond targeted medicine shows that the nano diamond targeted medicine has targeted selectivity; with human HepG2 and human HeLa as references, detection on cytotoxicity of the nano diamond targeted medicine by adopting an MTT method shows that the nano diamond targeted medicine has a targeted anti-tumour effect and selectivity for cancer cells; and study on a tumour inhibiting effect of the nano diamond targeted medicine in vivo by taking tumour-bearing mice as models shows that the nano diamond targeted medicine can be used for inhibiting tumours and has low toxic and side effects on DOX. Therefore, the nano diamond targeted medicine can be applied to preparation of an anti-tumour medicine.

Description

technical field [0001] The invention relates to targeted nano-medicine, in particular to a method for preparing transferrin-modified nano-diamond targeted drug, and the application of the drug prepared by the method in the preparation of anti-tumor drugs. Background technique [0002] At present, the methods used for cancer treatment mainly include surgery, radiotherapy, chemotherapy and biological therapy, etc., but all kinds of treatment methods bring great pain to patients, and the effect on patients with advanced cancer is not obvious, so the research is effective and has low harm targeted therapy is particularly urgent. In recent years, with the organic combination of nanoscience, life science, medicine and other disciplines, nanobiomedicine has become one of the emerging interdisciplinary subjects that attract people's attention. [0003] Due to the size effect of nanoparticles, it is mostly suitable for nanocarriers. Based on the high affinity of transferrin-transfer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61K47/48A61P35/00
CPCA61K31/704A61K47/66B82Y5/00A61K2300/00
Inventor 李英奇王志琴田志梅
Owner SHANXI UNIV
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