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A method for preparing total alkaloid extract and total flavonoids extract of camel grass from camel grass

A technology of camel grass and total alkaloids, which is applied in the directions of medical formula, drug delivery, plant raw materials, etc., can solve the problems affecting the research, development and utilization of the medicinal value of camel grass, and achieve the effect of increasing excretion

Inactive Publication Date: 2017-07-28
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no method for preparing the total alkaloid extract and the total flavonoids extract of Camelina from Camelina so far, which affects the research and development and utilization of the medicinal value of Camelina

Method used

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  • A method for preparing total alkaloid extract and total flavonoids extract of camel grass from camel grass
  • A method for preparing total alkaloid extract and total flavonoids extract of camel grass from camel grass
  • A method for preparing total alkaloid extract and total flavonoids extract of camel grass from camel grass

Examples

Experimental program
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Effect test

Embodiment

[0018] Example: Preparation of Camelina total alkaloid extract and Camelina total flavonoids extract

[0019] Take 100g of dried camel herb, cut into 2-4cm long pieces, crush into coarse powder, use 20 times the amount (ie: 2000mL) of 50% ethanol aqueous solution to soak for 2.5 hours, and then carry out reflux extraction 1.5 hours, a total of 3 extractions; combined extracts, filtered, and concentrated solvent in the filtrate, to obtain the total extract of Camelina;

[0020] Dissolve the above-mentioned total extract of Camelina chinensis in distilled water to prepare an aqueous solution with a concentration of 0.25g / mL; weigh 10g of D101 macroporous resin, put it on the column by wet method, and put it on the column at a flow rate of 2 times the volume of the macroporous resin column / hour. Sample, use the distilled water of 5 times of macroporous resin column volume to be 2 times of macroporous resin column volume / hour elution with elution flow rate, to remove polar impurit...

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Abstract

The invention discloses a method for preparing a peganum harmala linn total alkaloid extract and a peganum harmala linn total flavone extract from peganum harmala linn. The method comprises the following steps: performing reflux extraction on the peganum harmala linn by using an aqueous ethanol solution to prepare a peganum harmala linn total extract; dissolving in water to prepare an aqueous peganum harmala linn total extract solution, applying to a macroporous resin column, eluting by using distilled water for 1-5 column volumes so as to remove polar impurities; eluting by using 5-40% of the aqueous ethanol solution by volume for 5-20 column volumes, collecting eluate, and concentrating to obtain the peganum harmala linn total alkaloid extract; further eluting the macroporous resin column by using 45-80 % of the aqueous ethanol solution by volume for 10-30 column volumes, collecting eluate, and concentrating to obtain the peganum harmala linn total flavone extract. By the method, a convenient and quick way can be provided for researching, developing and utilizing the peganum harmala linn total alkaloid extract and the peganum harmala linn total flavone extract.

Description

technical field [0001] The invention relates to a method for preparing a total alkaloid extract and a total flavonoid extract of camel grass from camel grass, and belongs to the technical field of traditional Chinese medicine extraction. Background technique [0002] The linn is a plant of the genus Peganum in the family Zygophyllaceae (Zygophyllaceae). There are three species of this genus in my country, namely P.harmala L., P.nigelastrum Bge and P.multisectum (maxim.) Bobr. The dry aboveground part of it has the effects of dispelling mucus, reducing swelling and pain, promoting blood circulation and regulating menstruation, and is used for joint bone pain and menstrual obstruction. [0003] In the prior art, the research on Camelina mainly focuses on the chemical composition and pharmacological activity of Camelina, and also involves the research on the total alkaloid derivative preparation of Camelina, while the research on Camelina is relatively few, mainly focusing on ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185
CPCA61K9/0053A61K9/08A61K9/12A61K9/1652A61K9/2059A61K9/4866A61K36/185A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55
Inventor 王长虹程雪梅王峥涛刘伟温方方
Owner SHANGHAI UNIV OF T C M