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Bacterium for degrading herbicides and application thereof

A herbicide and fungicide technology, applied in the field of microorganisms, can solve problems such as slow natural degradation

Active Publication Date: 2015-03-04
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The degradation of herbicide residues in nature mainly depends on microorganisms in the soil, but the natural degradation is very slow

Method used

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  • Bacterium for degrading herbicides and application thereof
  • Bacterium for degrading herbicides and application thereof
  • Bacterium for degrading herbicides and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1. Isolation and identification of Rhizobium sp. 198-L-30CGMCC No. 9864

[0045] 1. Isolation of herbicide-degrading bacteria 198-L-30

[0046] 1. Add 10g of soil sample (collected from farmland contaminated by herbicides in Heihe, Heilongjiang, China) into 100ml of sterile water, shake culture for 30min to make a turbid solution. Take 1ml of the above turbid solution and add it to a test tube containing 9ml of sterile water and mix well (at this time, the dilution is recorded as 10 -1 ), then draw 1ml from this test tube and add it to another test tube containing 9ml of sterile water and mix well, and so on to make 10 -2 , 10 -3 , 10 -4 , 10 -5 Bacterial suspensions of different dilutions. Take 0.1ml of each dilution and evenly spread it on a nitrogen-free solid medium plate, and incubate at a constant temperature of 28°C for 2-3 days. After the colonies are formed, the strains are purified on a nitrogen-free solid medium plate.

[0047] 2. The preliminary screening...

Embodiment 2

[0067] Example 2. Quantitative determination of Rhizobium sp. 198-L-30CGMCC No.9864 degradation ability

[0068] 1. Drawing of the standard curve of Pusite determination

[0069] Accurately weigh 10.0 mg of Pusite standard (product of Fluka) into a 10mL volumetric flask, dissolve it with a small amount of methanol, place the volumetric flask in an ultrasonic bath and shake for 10min, then dilute to 10mL with methanol, shake well, and mix 1000mg / L Pu Shite mother liquor. Continue to dilute with methanol to prepare 20, 40, 60, 80, 100 mg / L series of Pu Shite standard solutions. High-performance liquid chromatography (HPLC) was used to determine the peak area of ​​different concentrations of Pursite standards, and the peak area was repeated 3 times. Use the Pste concentration as the abscissa and the peak area as the ordinate to draw the Pste standard curve, such as figure 1 Shown.

[0070] The detection conditions are as follows:

[0071] Detection system: Agilent 1100Series. Column...

Embodiment 3

[0081] Example 3. Quantitative determination of the ability of Rhizobium sp. 198-L-30CGMCC No. 9864 to degrade acetochlor

[0082] 1. Production of standard curve for acetochlor determination

[0083] Accurately weigh 10.0 mg of acetochlor standard (product of Fluka) into a 10 mL volumetric flask, dissolve it with a small amount of methanol, place the volumetric flask in an ultrasonic bath and shake for 10 minutes, then dilute to 10 mL with methanol, shake well, and mix 1000mg / L acetochlor mother liquor. Then it was diluted with methanol to obtain standard solutions with concentrations of 10, 20, 40, 60, 80, and 100 mg / L. High performance liquid chromatography (HPLC) was used to determine the peak areas of the standards of acetochlor at different concentrations, repeated three times. Using the concentration of acetochlor as the abscissa and the peak area as the ordinate, draw a standard curve of acetochlor, such as figure 2 Shown.

[0084] The detection conditions are as follows: ...

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Abstract

The invention discloses a bacterium for degrading herbicides and application thereof. The bacterium for degrading the herbicides is Rhizobium sp. 198-L-30 which has the collection number of CGMCC No. 9864 in the China General Microbiological Culture Collection Center. According to the Rhizobium sp. 198-L-30 with the collection number of CGMCC No. 9864 provided by the invention, the degradation rate on 100mg / L of imazethapyr in an inorganic salt culture medium within 7 days is 93.40 percent; the degradation rate on 50mg / L of acetochlor is 88.44 percent; the degradation rate on 100mg / L of chlorimuron-ethyl is 41.15 percent; and the degradation rate on 100mg / L of acifluorfen is 12.28 percent. According the strain, the herbicides such as imazethapyr, acetochlor, chlorimuron-ethyl and acifluorfen can be degraded.

Description

Technical field [0001] The present invention relates to a bacterium capable of degrading herbicides in the field of microorganisms and uses thereof. The herbicide can be at least one of Prosthet, acetochlor, chlorimuron-methyl and weeds. Background technique [0002] Pursuit, also known as imazethapyr-ammonium, English name Pursuit (imazethapyr-ammonium), chemical name (RS)-5-ethyl-2-(4-isopropyl-4-methyl-5-oxo-2) -Imidazolin-2-yl)nicotinic acid. Prost is an imidazolinone herbicide, a side chain amino acid synthesis inhibitor. It can be applied before or after germination. It can be applied to grass weeds in soybeans and other legumes farmland and some broad-leaved weeds such as amaranth , Polygonum, Chenopodium, Solanum, Xanthium, Barnyardgrass, Setaria, Crabgrass, Millet, etc. have excellent control effects. [0003] Acetochlor, the English common name acetochlor, the chemical name is 2-ethyl-6methyl-N-ethoxymethyl-α-chloroacetanilide. Acetochlor is a systemic amide herbicide ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/41A62D101/04A62D101/28
CPCA62D3/02A62D2101/04B09C1/10C12N1/20C12N1/205C12R2001/41
Inventor 高淼孙建光张磊孙颖飞
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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