Bacillus subtilis and application thereof in plant disease control

A technology for preventing and controlling Bacillus subtilis and plant diseases, applied in the field of microbial screening, can solve problems such as unstable disease prevention effect of Bacillus subtilis, and achieve strong bacteriostatic effect, obvious antagonistic effect and broad prospects.

Active Publication Date: 2015-03-11
山东蔚蓝绿源生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are abundant microbial species in the growth environment of plants, and there is great potential to develop beneficial bacillus, but the disease prevention effect of biocontrol bacillus is sometimes unstabl

Method used

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  • Bacillus subtilis and application thereof in plant disease control
  • Bacillus subtilis and application thereof in plant disease control
  • Bacillus subtilis and application thereof in plant disease control

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0014] Example 1 Screening and isolation of strains

[0015] 1. Preparation of soil sample diluent:

[0016] Retrieve the soil from the vegetable greenhouse in Beichengyang Village, Haiyang, weigh 0.5g of the soil sample and dissolve it in 4.5ml of sterile water to make a 1:10 soil solution, then draw 0.5ml of the soil solution from it and place it in 4.5ml of sterile water. Make a soil solution of 1:100, by analogy with this method, prepare 1:10 6 -10 7 Soil dilution solution.

[0017] 2. Strain isolation and purification:

[0018] Take 3-4 dilutions of appropriate concentration and spread 0.1ml evenly on the nutrient agar medium; after incubating at 37°C for 2 days, take it out, pick a single colony and continue to separate and purify it until the color of the colony grown on each plate is consistent. It is a single strain.

[0019] Using the above method, the applicant screened a total of 12 strains, named FX1, FX2, FX3,...FX12.

[0020] 3. Pathogen confrontation experiment:

[0021]...

Example Embodiment

[0022] Example 2 Verification experiment on the control effect of cucumber powdery mildew

[0023] 1. Preparation of bacterial liquid

[0024] The four selected strains FX1, FX4, FX6, and FX10 were respectively inoculated into nutrient broth medium and cultured at 30°C for 2-3 days, and the content of viable bacteria was 10 8 -10 9 cfu / ml bacterial liquid.

[0025] 2. Experimental process

[0026] 1) Experimental group: Four strains of FX1, FX4, FX6, and FX10 were sprayed on cucumber leaves with the same dosage respectively; each strain was set up with three treatment groups, each treatment group was randomly selected two rows of cucumbers, a total of 100 trees, each Set protection lines between processing groups.

[0027] In the middle of cucumber growth, the above bacterial liquid was diluted 500 times with water and sprayed evenly on the cucumber leaves of each treatment group; each spraying interval was 7 days, and used three times in a row; 10 days after the last spraying of the b...

Example Embodiment

[0037] Example 3 Identification of FX1 strain

[0038] 1) Physiological and biochemical characteristics of FX1 strain:

[0039] The colony is flat, the surface is rough and opaque, dirty white, the spore size is 0.6-0.9×0.9-1.4μm, elliptical or columnar, mesophytic or nearly mesophytic; Gram reaction is positive, catalase reaction is positive, VP reaction is positive, Starch hydrolysis is positive, gelatin liquefaction is positive, casein is decomposed, the growth temperature range is 25-40℃, and the pH range is 5-8.

[0040] 2) Molecular biological identification of FX1 strain:

[0041] The FX1 strain obtained from the above screening was identified by molecular biology method, and its 16s rDNA sequence was measured, and the blast comparison was performed in the GenBank nucleic acid database. Combining the biological characteristics of the FX1 strain and the 16srDNA comparison result, the applicant confirmed that the FX1 strain is Bacillus subtilis (Bacillus subtilis), named Bacillu...

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Abstract

The invention provides a bacillus subtilis and an application thereof in plant disease control, relates to the technical field of microorganism screening, and specifically provides a bacillus subtilis FX1 screened from the soil of a vegetable greenhouse, wherein the preservation number of the bacillus subtilis FX1 is CCTCC NO.M2014563. The strain has very strong antibacterial effect and can be widely applied to the plant disease control. The cucumber powdery mildew control efficiency of the strain is up to 95.5%, the strawberry powdery mildew control efficiency of the strain is up to 94.8%, and the grape brown spot control efficiency is up to 83.2%. Besides controlling the plant powdery mildew and brown spot, the bacillus subtilis FX1 further has remarkable antagonism to plant yellow rot and fruit rot caused by fusarium and rhizoctonia solani, the control efficiency is up to more than 80%, and the application prospect is wide.

Description

technical field [0001] The invention relates to the technical field of microbial screening, in particular to a bacillus subtilis and its application in plant disease control. Background technique [0002] With the continuous increase of plant species and the continuous diversification of plant cultivation methods, plant diseases are becoming more and more serious. However, traditional chemical control methods have many disadvantages, such as: the extensive use of chemical pesticides has caused serious environmental pollution, pesticide residues in agricultural products It is also becoming more and more serious, and the rate of bacterial resistance to antibiotics is getting faster and faster, which has a series of harmful effects on people's lives. In today's low-carbon economy, environmental protection and conservation-oriented society, seeking new technologies that can protect human life and increase production is very suitable for the development of today's society. [00...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125
CPCA01N63/00C12N1/205C12R2001/125
Inventor 苑伟伟王鹏唐波朱彩彩甄丽刘鲁民
Owner 山东蔚蓝绿源生物科技有限公司
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