A method for non-invasive determination of tumor receptor density in nude mice based on optical imaging

Abstract

The invention relates to an optical-image-based non-invasive naked mouse tumor receptor density determining method. Receptor detection expressing means and methods in existing scientific experiments can be only used for qualitative or half quantitative measurement but cannot calculate receptor density values accurately. The method includes the steps of 1, establishing a naked mouse subcutaneous tumor model; 2, injecting an optical mark target probe to perform dynamic optical acquisition; 3, marking an ROI (region of interest); 4, performing singular value decomposition; 5, fitting data to obtain a BP value; 6, calculating an available receptor density. The optical-image-based non-invasive naked mouse tumor receptor density determining method is a method by which available receptor density information of tumor regions can be obtained without obtaining living tissues invasively, thereby being simpler and more convenient and accurate than previous methods.

Description

technical field [0001] The invention belongs to the technical field of medical imaging, in particular to a method for non-invasively measuring tumor receptor density in nude mice based on optical images. Background technique [0002] Receptor is a kind of special protein that exists in the cell membrane or in the cell, and can bind to specific signal molecules outside the cell to activate a series of biochemical reactions in the cell, so that the cell can produce corresponding effects on external stimuli. Biologically active substances that bind to receptors are collectively referred to as ligands. When a receptor binds to a ligand, a molecular conformational change occurs, thereby causing cellular responses, such as mediating intercellular signal transduction, intercellular adhesion, and endocytosis. [0003] It is known from the pathogenesis of tumors that the expression state of tumor-specific receptors on the surface of tumor cells is different from that of normal cells...

AI Technical Summary

Problems solved by technology

However, this method obviously has disadvantages such as cumbersome operation process, invasive tissue slices must be obtained for in vitro measurement, and it cannot correctly reflect the state of the receptor in vivo and the density of the receptor.

[0007] In order to avoid the defect that traditional methods cannot correctly reflect the state and density of receptors in vivo due to in vitro measurements, the researchers also proposed a radiolabeled ligand assay method based on positron emission tomography (PET)

Although this isotope tracer technique can accurately measure the available receptor density in the tumor area of ​​nude mice in vivo, it requires the labeling of radioactive elements [11C] with a short half-life, and the technical threshold is high, the steps are cumbersome, and there is radioactive radiation.

[0008] To sum up, for the available receptor density detection of tumors in nude mice, the existing technologies and methods are difficult to overcome the shortcomings of invasive operation, inaccurate quantification, high equipment technical threshold, and easy to cause radioactive contamination.

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