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Application of kaempferol in medicines for inhibiting rejection reaction of receptor on organ transplantation

A technology of rejection reaction and kaempferol, applied in the field of medicine, can solve problems such as high cost, and achieve the effects of wide application range, reduction of dosage and duration of use, and reduction of side effects

Inactive Publication Date: 2015-03-25
GUANGDONG HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term use of immunosuppressants is not only expensive, but also prone to many side effects, such as infection, tumor, digestive system, urinary system and other side effects

Method used

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  • Application of kaempferol in medicines for inhibiting rejection reaction of receptor on organ transplantation
  • Application of kaempferol in medicines for inhibiting rejection reaction of receptor on organ transplantation
  • Application of kaempferol in medicines for inhibiting rejection reaction of receptor on organ transplantation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Kaempferol significantly prolongs the survival time of islet grafts

[0031] The above 6 groups of mice with different treatments were tested for the survival time of islet transplantation, and the test results were as follows: figure 1 and shown in Table 1.

[0032] Table 1 The mean time of kaempferol itself or combined with cyclosporine to promote the survival of islet grafts in mouse recipients

[0033]

[0034] From figure 1 and as can be seen in Table 1:

[0035] The average survival time of islets of mice in the kaempferol group (27 days) was significantly longer than that of the control group (14 days), n=7-8, P=0.011; indicating that kaempferol can significantly prolong the survival time of islet grafts, Reduced rejection of organ transplantation in recipient mice;

[0036] The average survival time of islets of mice in the low-dose cyclosporine group (CsA 6mg) (35 days) was significantly longer than that of the control group (14 days), n=7-8,...

Embodiment 2

[0040] Example 2 Kaempferol increases regulatory T lymphocytes (CD4 + FoxP3 + Tregs) number

[0041] After feeding the transplanted and differently treated mice in 6 groups for 4 weeks, the number of regulatory T lymphocytes in the mouse kidneys (including islet transplants) and lymph nodes was quantitatively analyzed by flow cytometry. The analysis results were as follows: figure 2 shown, from figure 2 It can be seen in:

[0042] CD4 in kidney and lymph nodes of Kaempferol mice + FoxP3 + The number of Tregs were (6.4±0.6)×10 4 pcs, (3.4±0.4)×10 4 , which were higher than (4.7±0.4)×10 in the control group 4 (P=0.024), (2.2±0.2)×10 4(P=0.022); indicating that kaempferol can significantly induce and up-regulate the number of regulatory T lymphocytes in mouse kidney and lymph nodes, and reduce the rejection of mouse recipients to organ transplants;

[0043] CD4 in kidney and lymph nodes of mice in low dose cyclosporine group (CsA 6mg) + FoxP3 + The number...

Embodiment 3

[0047] Example 3 Kaempferol inhibits IL-6 gene expression in islet transplantation

[0048] Six groups of mice with different treatments after transplantation were fed for 4 weeks, and the expression of IL-6 gene in the islet grafts of recipient mice was detected. The detection results are as follows: image 3 shown, from image 3 It can be seen in:

[0049] The relative expression level of IL-6 mRNA in the kaempferol group was 1.7 ±0.2, while the relative expression level of IL-6 in the control group was 5.0 ±0.6, and kaempferol significantly down-regulated the expression level of IL-6 mRNA (P=0.006);

[0050] The relative expression of IL-6 mRNA in the kaempferol + low-dose cyclosporine group (Kaempferol + CsA 6mg) was 1.0 ± 0.2, and the relative expression of IL-6 in the low-dose cyclosporine group (CsA 6mg) was 4.5 ± 0.4 , the kaempferol + low-dose cyclosporine group (Kaempferol + CsA 6mg) significantly down-regulated the expression level of IL-6 mRNA compared with the...

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Abstract

The invention discloses an application of kaempferol in medicines for inhibiting rejection reaction of a receptor on organ transplantation. The invention discloses pharmacological effects of kaempferol in inhibiting rejection reaction of organ transplantation in the body of a receptor for the first time; and moreover, optimal effects can be achieved when kaempferol is combined with low-dose cyclosporin for a short time, and long-term survival of an islet graft in the receptor can be induced, so that various side effects caused by using full-dose cyclosporin for a long time can be avoided, the economic burden of patients brought by long-term anti-rejection treatment can also be lightened, and the long-term high-quality life of organ transplantation patients can be further facilitated. The invention also discovers an immune adjustment action mechanism of kaempferol, namely that kaempferol can be used for significantly increasing the number of regulatory T lymphocytes in the body of the receptor and reducing the gene expression of an islet graft IL-6 in the body of the receptor, so that the effects of resisting inflammation and resisting rejection reaction can be achieved.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the application of kaempferol in drugs for inhibiting organ transplantation rejection by recipients. Background technique [0002] Organ transplantation is widely used in clinical treatment to save patients suffering from various terminal organ diseases, such as end-stage heart, kidney, liver, lung and other diseases. my country has become the second largest organ transplant country in the world. There are only more than 9,000 organ transplant operations per year, but more than 300,000 organ failure patients need transplants each year. Therefore, there is a huge demand and development potential for organ transplants in my country. However, severe rejection often occurs after transplantation, and patients need to continue to use immunosuppressive drugs to suppress the recipient's rejection of the graft. Cyclosporine is one of the most commonly used immunosuppressants, but long-term use of i...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61K38/13A61P37/06
CPCA61K31/352A61K38/13
Inventor 戴振华刘旭生曾玉群卢富华邹川卢钊宇苏国彬吴禹池张腊吴一帆
Owner GUANGDONG HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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