42 results about "Lymphocyte Numbers" patented technology

The invention provides a compound feed additive for enhancing broiler chicken immunity. The compound feed additive is prepared by 28-50 parts of rhodiola glucoside, 15-27 parts of hydrochloric acid berbamine, 9-22 parts of pueraria root extract, 7-20 parts of schisanhenol and 6-16 parts of eupolyphaga powder. The compound feed additive can effectively promote growth of broiler chicken immune organs, obviously improve the level of immune globulin IgG in serum, the lymphocyte transformation rate and the leukocyte phagocytic ability, increase the number of T-lymphocytes and enhance the body liquid and the cellular immune function of broiler chickens, and therefore the incidence rate of diseases and the use frequency of antibiotic and other medicine are reduced, and direct economic and social benefits are achieved.

A method to characterize a T-cell response of a final population of T-lymphocytes resulting from the co-incubation of an initial population of T lymphocytes with a composition of antigen-presenting cells (APCs), said method comprising the steps of a) simultaneous measuring on a single cell basis at least two parameters: (i) proliferation of T lymphocytes and (ii) presence of a T cell antigen receptor on the surface of T lymphocytes and / or presence of at least one biological molecule produced by T lymphocytes, and the attribution of a positive or a negative value to each of these parameters, and b) classification of the final T-lymphocytes population into 2n different subsets of T lymphocytes, n being the number of parameters, each subset being characterized by a positive or a negative value respectively to each parameter and the determination of the proportion of T lymphocytes present in each subset with respect to the number of T lymphocytes in the final population, with said proportion being characteristic of the T-cell response. Use of the method for batch release assay and potency assay.

The invention relates to use of compound pachymic acid in the pharmaceutical field. The product has the functions of decreasing the rejection reaction of a receptor to a graft after a transplant operation, protecting the stability of haemolymph cell mitochondrial membrane potential at the periphery of the receptor, reducing the apoptosis percentage of haemolymph cells at the periphery, stabilizing the quantity percentage of CD8 + lymph cells at the periphery, and reducing the apoptosis percentage of the haemolymph cells at the periphery and regulating the quantity percentage of CD4 + and CD8 + lymph cells under nontransplant conditions and so on, is used to reduce graft rejection reactions as well as regulate and stabilize the quantity, the proportion and the function of the lymph cells of organisms during the transplant of organs, tissues and cells of animals and human beings and so on.

The present disclosure provides methods for measuring the number of CD4+ OX40+ Foxp3+ lymphocytes in a sample containing cancer cells and lymphocytes obtained from a subject by labeling lymphocytes that show CD4 expression in the sample, then labeling lymphocytes that show OX40 expression in the sample, then labeling lymphocytes that show Foxp3 expression in the sample, then measuring the number of CD4+ OX40+ Foxp3+ lymphocytes in the sample. Further provided are methods for determining the prognosis of a subject, predicting responsiveness of a subject having cancer to an OX40 agonist treatment, and methods for treating or delaying progression of cancer based on the number of CD4+ OX40+ Foxp3+ lymphocytes in a sample.

A method for increasing the number of CD4+ T-lymphocytes in the serum of a subject in need of such treatment comprising administering to the subject a pharmaceutical composition comprising an amount of an L-isomer of β-lactam effective to increase the number of CD4+ T-lymphocytes in said patient's serum.

The invention provides a Chinese patent medicine for treating spleen deficiency syndrome postoperative fatigue syndrome and a preparation method thereof. Every 100g of the Chinese patent medicine is prepared from 20-50g of radix aucklandiae,10-30g of fructus amomi, 20-50g of atractylodes macrocephala koidz, 30-70g of prepared fingered citron, 20-50g of steamed pericarpium citri reticulatae, 20-50gof cooked codonopsis pilosula, 20-50g of poria cocos, 10-30g of honey-fried licorice root, 10-30g of ginger processed pinellia, 20-50g of radix salviae miltiorrhizae, 12-35g of sun-dried ginseng, 30-70g of coix seeds, 30-70g of caulis spatholobi, 30-70g of Chinese dates, 20-50g of capillary wormwood, 20-50g of endothelium corneum gigeriae galli and 20-50g of radix dipsaci. Clinical experiments prove that when the Chinese patent medicine is used for treating the spleen-deficiency syndrome postoperative fatigue syndrome, the number of T lymphocytes of a patient can be effectively increased, thepostoperative fatigue degree and the spleen-deficiency syndrome of the patient are improved, and the overall treatment effect on the disease is improved. Particularly, compared with a decoction usingthe same prescription, the Chinese patent medicine is better in curative effect due to the fact that medicinal components in medicinal materials can be better extracted and retained by means of the preparation method.

The invention discloses a feed additive for preventing or alleviating endotoxin infection of piglets after weaning and a preparation method thereof, belonging to the field of feed additives. The invention firstly discloses a feed additive for preventing or alleviating endotoxin infection of piglets after weaning, which comprises the following components: baicalin, taurine and lipoic acid. The invention also discloses a method for preparing the feed additive, which includes: weighing each component according to the weight ratio, and mixing to obtain. The feed additive of the present invention can significantly reduce the blood endotoxin content of piglets caused by weaning stress, effectively increase the number of red blood cells, the total number of white blood cells and the number of lymphocytes of weaned piglets, improve the vitality and immune resistance of piglets before and after weaning, and promote the growth and development of weaned piglets. growth, and effectively alleviate the stress brought by weaning to piglets; in addition, the feed additive of the present invention has definite anti-diarrhea effect.

The invention relates to an application of a compound pachymic acid in the field of pharmacy. With the product, rejection reaction against transplant after transplant operation can be reduced, peripheral blood lymphocyte mitochondrial membrane potential stability can be protected; peripheral blood lymphocyte apoptosis percentage can be reduced, peripheral blood CD8+ lymphocyte number percentage can be stabilized, and so on. Under a non-transplant condition, peripheral blood lymphocyte percentage can be reduced, CD4+ and CD8+ lymphocyte number percentage can be regulated, and so on. When the compound is used for treating, transplant rejection reactions of transplantations of animal and human organ, tissue, cell, and the like can be reduced; and body lymphocyte number, proportion, and function can be regulated and stabilized.

This invention discloses a detecting method for immune status of T-cell of hepatitis B patients. It is to use artificial synthesized polypeptide to stimulate the peripheral blood lymphocyte cultivated out of hepatitis B patients; to detect the number of lymphocyte with Gamma interferon in simulated body by use of enzyme associated immune blob experimental method, so to determine immune status of T-cell of hepatitis B patients. In normal matched group, there is barely no non-specific blot, and the stability of biological activity of synthesized polypeptide between batches is greatly increased and errors between batches are greatly induced. Synthesized polypeptide can be kept well in the temperature of four degree for six months without apparent reduction of activity, which is very helpful for the use of reagent box.

The invention discloses the application of the hepcidin antagonist LDN193189 and its derivatives in the preparation of medicines, belongs to the field of medical technology, and provides the application of the hepcidin antagonist LDN193189 and its derivatives in the preparation and treatment of bone marrow type acute radiation sickness and radiotherapy-induced blood cell drop New uses of applications in disease medicine. Intraperitoneal injection of lethal doses of irradiated mice with LDN193189 and its derivatives can increase the survival rate of irradiated mice to 40% (120 days), and the number of peripheral blood leukocytes and lymphocytes increased significantly; The number of cells was significantly restored; the survival rate of splenic lymphocytes was significantly restored; the number of splenic lymphocytes was significantly increased (HE section). LDN193189 and its derivatives have a good effect on the treatment of bone marrow type acute radiation sickness and radiotherapy-induced cytopenia.