Three-dimensional biological printing device and method

Abstract

The invention discloses a three-dimensional biological printing device and a three-dimensional biological printing method. The three-dimensional biological printing device comprises a printing sprayer, a first temperature control module, an auxiliary sprayer, a forming platform and a semiconductor chilling plate, wherein the printing sprayer is used for loading a sol state thermo-sensitive type material solution, and spraying and printing the material solution to the forming platform; the first temperature control module is arranged on the periphery of the printing sprayer and used for maintaining temperature of the material solution; the bottom of the forming platform is provided with the semiconductor chilling plate; the auxiliary sprayer is used for loading a chemical crosslinking agent and spraying the chemical crosslinking agent to a layer of material solution on the forming platform in an atomizing manner; while the device is used for printing, the chemical crosslinking agent is sprayed to the layer of material solution to the forming platform in the atomizing manner after a layer of sol state material solution is sprayed. Through the adoption of the three-dimensional biological printing device and the three-dimensional biological printing method disclosed by the invention, the printed three-dimensional structure is good in layering effect, and the structure is stable.

Description

【Technical field】 [0001] The invention relates to three-dimensional printing technology in tissue engineering, in particular to a three-dimensional bioprinting device and method. 【Background technique】 [0002] Bio-3D printing technology is to use the principle and method of 3D printing to print biological materials (including natural biological materials and synthetic biological materials) or cell solutions into a designed three-dimensional structure, in which the technology of cell solution as printing material is also called three-dimensional printing of cells technology. Currently, the methods for realizing biological three-dimensional printing include: inkjet printing technology, printing based on laser technology, and printing method based on extrusion (rapid prototyping). Among them, extrusion-based printing technology is currently the most suitable technology for building complex 3D structures. [0003] In the printing process, how to solidify the material is an im...

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Problems solved by technology

However, in this way, although the curing effect is enhanced, the three-dimensional structure is easily contaminated during the transfer process. If it is a three-dimensional structure of cells, the cell survival rate is low, which is very unfavorable for three-dimensional printing of cells.

[0007] In addition to their respective shortcomings, the curing printing under the above methods is not suitable for printing three-dimensional biological structures that require better layering effects, because the layering effects of the three-dimensional structures obtained by the above printing and curing are not very good, and the structure Stability is difficult to guarantee

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