Culture medium and culture method for Drechmeria coniospora ARSEF 6962
A technology of conical excavation and culture medium, applied in the field of microorganisms, can solve the problems of low yield and long cultivation period, and achieve the effects of increased biomass, shortened cultivation period and low equipment requirements
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Embodiment 1
[0045] Example 1 Study on the dosage of animal liver and kidney
[0046] The solid medium (see Table 1) with different content of animal liver and kidney was prepared, and the growth cycle of the M. conezii strain inoculated in the medium was determined.
[0047] Other components in each culture medium are NaCl and sterile water. In every 100 mL of culture medium, 0.5 g of NaCl and 1.5 g of agar are contained, and the rest is sterile water.
[0048] Table 1
[0049]
[0050] Experimental results and analysis:
[0051] The bacterial strain cultured on the culture medium of the present invention can be seen to be covered with fungal mycelia on the plate culture medium after 4-5 days of culture, the mycelium is relatively dense, and the sporulation stage is relatively concentrated.
[0052] However, the literature (HANS-BORJE JANSSON, *A.JEYAPRAKASH, AND BERT M.ZUCKERMAN.APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Mar.1985, p.552-555) was used to cultivate bacterial strains on C...
Embodiment 2
[0058] Example 2 Research on the dosage ratio of animal liver and kidney in the culture medium
[0059] The growth cycle of M. conezii in culture medium with different proportions of liver and kidney was determined and analyzed. The measuring method of culture method and result is the same as embodiment 1.
[0060] The liver content in A fixed medium is 10%, and the kidney content 1% (LK-100-10), 2% (LK-100-20) and 5% (LK-100-50) are analyzed;
[0061] Kidney content was 10% in B fixed medium, and liver content 1% (LK-10-100), 2% (LK-20-100) and 5% (LK-50-100) were analyzed.
[0062] Medium composition: see Table 2
[0063] Table 2
[0064]
[0065]
[0066] Experimental results and analysis:
[0067] The growth medium of various liver-kidney ratios can significantly promote the growth of the strain. When the contents of liver and kidney in the medium were the same, the growth period of the strain was the shortest. Both the growth time of M. conei mycelia and the g...
Embodiment 3
[0072] Example 3 Addition of Animal Liver and Kidney Functional Effect Comparison in Medium
[0073] The purpose of this experiment is to study the growth-promoting function of M. conezii by adding only the medium prepared by liver or kidney alone. The effects of liver or kidney are compared by measuring and analyzing the growth cycle of the strains.
[0074] Add liver to the fixed medium without adding kidney, wherein the contents of kidney and liver are 0.1% (L-1), 1% (L-10), 5% (L-5) and 10% (L-100) for analysis;
[0075] B. Kidney was added without liver in the fixed medium, and the content of kidney was 0.1% (K-1), 1% (K-10), 5% (K-5) and 10% (K-100) for analysis.
[0076] Medium composition: see Table 3
[0077] table 3
[0078]
[0079]
[0080] Experimental results and analysis:
[0081] Compared with the contents of both liver and kidney in the culture medium being 10%, the growth period of the strains in the culture medium is affected by the single addition...
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