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One-component TMB developing solution for enzyme-linked immunosorbent assay

An enzyme-linked immune reaction and enzyme-linked immune reagent technology, applied in the field of one-component TMB chromogenic solution, can solve the problems of troublesome operation and poor stability of the chromogenic solution, and achieves avoiding changes in concentration, good stability and sufficient dissolution. Effect

Inactive Publication Date: 2015-03-25
CHENGDU VERO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a single-component TMB chromogenic solution for enzyme-linked immunoreaction, to overcome the problems of troublesome operation and poor stability of the existing chromogenic solution

Method used

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  • One-component TMB developing solution for enzyme-linked immunosorbent assay

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Embodiment 1

[0021] A one-component TMB chromogenic solution for ELISA, each 1000mL of chromogenic solution includes the following components: citric acid 7g, disodium hydrogen phosphate 16g, sodium perborate 0.1g, glycerin 20ml, EDTA 1g, TMB 5mg , 10 mL DMSO.

Embodiment 2

[0023] A one-component TMB chromogenic solution for ELISA, each 1000mL chromogenic solution includes the following components: citric acid 7g, disodium hydrogen phosphate 16g, sodium perborate 0.1g, glycerin 20ml, EDTA 2g, TMB 5mg , 10 mL DMSO.

Embodiment 3

[0025] A one-component TMB chromogenic solution for ELISA, each 1000mL of chromogenic solution includes the following components: citric acid 7g, disodium hydrogen phosphate 16g, sodium perborate 0.1g, glycerin 20ml, EDTA 3g, TMB 5mg , 10 mL DMSO.

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Abstract

The invention discloses a one-component TMB developing solution for enzyme-linked immunosorbent assay. Every 1000mL developing solution comprises the following components: 7-10 g of citric acid, 16-20 g of disodium hydrogen phosphate, 0.1-0.5 g of sodium perborate, 20-30 ml of glycerinum, 5-40 mg of TMB and 10-40 ml of DMSO; the one-component TMB developing solution which is good in oxidation effect also comprises 1-3 g of EDTA. Through using the one-component TMB developing solution in the range in the developing state of the enzyme-linked immunosorbent assay, the condition of mixing a solution A and a solution B during the use of the TMB developing solution is avoided, so that the operation time is decreased, the concentration change of the effective developing components of the TMB developing solution is avoided and the sensitivity of the TMB developing solution is improved; the TMB solvent used by the one-component TMB developing solution is DMSO, and compared with the solvent ethyl alcohol, the TMB dissolution speed of the DMSO is high, the dissolution is sufficient and the stability of the dissolved solution is better; through the addition of the EDTA, the stability of the TMB solvent can be further improved.

Description

technical field [0001] The invention relates to the field of TMB chromogenic solution, in particular to a single-component TMB chromogenic solution for enzyme-linked immunoreaction. Background technique [0002] TMB is a peroxidase substrate used in ELISA reactions. The substrate produces a soluble light blue end product that can be read on a spectrophotometer at 370 or 635 nm. It is suitable for quantitative and qualitative analysis in the chromogenic stage of ELISA. The principle of the test is: when the enzyme labeled on the antibody or antigen reacts with TMB, hydrogen peroxide, etc. in the chromogenic solution, the solution turns from colorless to yellow after adding the stop solution, and the depth of yellow reflects the amount of antigen-antibody binding in the ELISA system , so as to judge the amount of a certain antigen or antibody. Measure the absorbance value with a microplate reader to judge the degree of immune reaction. [0003] Most of the TMB chromogenic...

Claims

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Application Information

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IPC IPC(8): G01N33/543
CPCG01N33/543
Inventor 张志新叶正义杨秦何远立
Owner CHENGDU VERO BIOTECH
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