One-component TMB developing solution for enzyme-linked immunosorbent assay

Abstract

The invention discloses a one-component TMB developing solution for enzyme-linked immunosorbent assay. Every 1000mL developing solution comprises the following components: 7-10 g of citric acid, 16-20 g of disodium hydrogen phosphate, 0.1-0.5 g of sodium perborate, 20-30 ml of glycerinum, 5-40 mg of TMB and 10-40 ml of DMSO; the one-component TMB developing solution which is good in oxidation effect also comprises 1-3 g of EDTA. Through using the one-component TMB developing solution in the range in the developing state of the enzyme-linked immunosorbent assay, the condition of mixing a solution A and a solution B during the use of the TMB developing solution is avoided, so that the operation time is decreased, the concentration change of the effective developing components of the TMB developing solution is avoided and the sensitivity of the TMB developing solution is improved; the TMB solvent used by the one-component TMB developing solution is DMSO, and compared with the solvent ethyl alcohol, the TMB dissolution speed of the DMSO is high, the dissolution is sufficient and the stability of the dissolved solution is better; through the addition of the EDTA, the stability of the TMB solvent can be further improved.

Description

technical field [0001] The invention relates to the field of TMB chromogenic solution, in particular to a single-component TMB chromogenic solution for enzyme-linked immunoreaction. Background technique [0002] TMB is a peroxidase substrate used in ELISA reactions. The substrate produces a soluble light blue end product that can be read on a spectrophotometer at 370 or 635 nm. It is suitable for quantitative and qualitative analysis in the chromogenic stage of ELISA. The principle of the test is: when the enzyme labeled on the antibody or antigen reacts with TMB, hydrogen peroxide, etc. in the chromogenic solution, the solution turns from colorless to yellow after adding the stop solution, and the depth of yellow reflects the amount of antigen-antibody binding in the ELISA system , so as to judge the amount of a certain antigen or antibody. Measure the absorbance value with a microplate reader to judge the degree of immune reaction. [0003] Most of the TMB chromogenic...

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a single-component TMB chromogenic solution for enz

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