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Preparation method of egg yolk antibody against Peste des petits ruminants virus

A technology of Peste des petits ruminants and egg yolk antibodies, which is applied in the direction of antiviral immunoglobulins and egg-derived immunoglobulins, can solve problems such as unsatisfactory treatment effects, and achieve good application prospects, rapid treatment effects, and high specificity Effect

Active Publication Date: 2019-03-26
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since there is no specific drug for viral diseases, clinical treatment mainly adopts symptomatic treatment and antibiotics to prevent complications, and the treatment effect is not ideal

Method used

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  • Preparation method of egg yolk antibody against Peste des petits ruminants virus
  • Preparation method of egg yolk antibody against Peste des petits ruminants virus

Examples

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Embodiment 1

[0022] Example 1 Peste des petits ruminants virus egg yolk antibody and its preparation method

[0023] 1. Preparation of Vaccines for Immunization

[0024] 1.1 Propagation of Peste des petits ruminants virus and preparation of antigen

[0025] Inoculate 3 bottles of Vero cells with the virus liquid of Peste des petits ruminants, and inoculate 1ml of the virus liquid in each bottle of cells, the area of ​​the bottom of the bottle is 25cm 2 , cover the bottle cap, turn the horizontal bottle tube gently to make the virus liquid fully contact with the whole cell monolayer, adsorb at 37°C for 40 minutes, shake it every 10 minutes during this period, do not need to suck and discard the virus liquid, directly add 2 % fetal bovine serum DMEM (Dulbecco; s Modified Eagle Medium) cell maintenance medium 5ml, set up cell control at the same time, at 37 ℃ CO 2 cultured in an incubator. After 24 hours, the maintenance solution was discarded and the medium was changed. Observe the cell ...

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Abstract

The invention provides a preparation method of a peste des petits ruminants virus (PPRV) yolk antibody. The preparation method of the PPRV yolk antibody comprises the following steps: (1) preparing a peste des petits ruminants virus antigen by using peste des petits ruminants viruses, inactivating the peste des petits ruminants virus antigen, mixing the inactivated peste des petits ruminants virus antigen with Freund's adjuvant, immunizing a laying hen with the mixture, collecting eggs, and preserving at the temperature of 4 DEG C; and (2) purifying by using octanoic acid to obtain the peste des petits ruminants virus yolk antibody. The peste des petits ruminants virus yolk antibody prepared by adopting the preparation method has the advantages of significant effect, high specificity, definite curative effect and low production cost, can take effect quickly, can effectively inhibit the attack of the peste des petits ruminants viruses and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of veterinary biological products, and in particular relates to a preparation method of egg yolk antibody of Peste des petits ruminants virus. Background technique [0002] Peste des Petits Ruminants (PPR), an acute and severe infectious disease caused by Peste des Petits Ruminants Virus (PPRV), is a legally notifiable infectious disease stipulated by FAO / OIE. It is considered to be one of the most important severe infectious diseases in the world, belonging to the genus Morbollivirus of the family Paramyxoviriade (Paramyxoviriade), and mainly infects goats, sheep and wild small ruminants. The disease is characterized by fever, stomatitis, and diarrhea. In 1942, PPR was reported for the first time in Côte d'Ivoire, West Africa. Peste des petits ruminants was reported for the first time in 2007 in the Tibet Autonomous Region of my country, followed by PPR outbreaks in Tibet in 2008 and 2010, in Yili, Xinjiang in Dece...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/10C07K16/02
Inventor 李刚隋修锟李铁梁琳
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI