Multi-color multi-parameter portable flow cytometer
A flow cytometer and multi-parameter technology, which is applied in the fields of life science and medicine, can solve the problems of few types of excitation light sources, few detection channels, and large volume, meet the requirements of reducing sample volume and sample concentration, and improve instrument measurement accuracy , The effect of reducing the volume of the instrument
Inactive Publication Date: 2015-04-01
CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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[0004] In order to solve the problems of large volume, high cost, few types of excitation light sources, and few detection channels in the existing flow cytometer, the present invention provides a multi-color flow cytometer with small volume, convenient portability, multiple excitation light sources and multiple detection channels. Multi-parameter portable flow cytometer
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[0038] The present invention will be described in further detail below in conjunction with the accompanying drawings.
[0039] Such as figure 1 As shown, a multi-color multi-parameter portable flow cytometer of the present invention includes an LED light source group 1, a light source shaping system 2, a flow chamber 3, a liquid flow drive system 4, a forward channel signal acquisition system 5, and a side channel signal acquisition system. Acquisition system 6 and data analysis system 7.
[0040]The LED light source group 1 is composed of multiple light-emitting chips with different wavelengths, and can emit light with multiple different wavelengths. Controlling the working mode of the light-emitting chip can make the LED light source group 1 be in different working modes. A single light-emitting chip works alone or multiple light-emitting chips work simultaneously at a certain frequency. The number of light-emitting chips in the LED light source group 1 is preferably 4. Th...
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Abstract
The invention discloses a multi-color multi-parameter portable flow cytometer, relates to the field of life and medical science, and solves the problems that a conventional flow cytometer is large in size, high in cost, little in type of excitation light sources and small in number of detection channels. The multi-color multi-parameter portable flow cytometer comprises an LED light source group, a light source shaping system, a flowing chamber, a liquid flow driving system, a forward path signal acquisition system, a lateral path signal acquisition system and a data analysis system, wherein the LED light source group is used for emitting multiple rays with different wavelengths; the rays are shaped by the light source shaping system to emit rectangular light spots; an object to be detected is conveyed into the flowing chamber through the liquid flow driving system and passes through fluid channels in sequence to reach a laser detection area; the object to be detected is radiated by the rectangular light spots to excite scattered light and fluorescent light of different wave bands; the forward path signal acquisition system is used for converting a scattered light signal into an electric signal; the lateral path signal acquisition system is used for converting scattered light signals and fluorescent light signals into electric signals; the data analysis system is used for receiving the electric signals and outputting an analysis result. The multi-color multi-parameter portable flow cytometer is small in size and convenient to carry and has multiple excitation light sources and multiple detection channels.
Description
technical field [0001] The invention relates to the technical fields of life science and medicine, in particular to a multi-color and multi-parameter portable flow cytometer. Background technique [0002] Flow cytometry can perform multi-parameter, quantitative analysis or sorting of cells or biological particles in a fast-flowing state. It has been widely used in basic research such as cell biology, immunology, physiology, and molecular biology. It is used in medical clinical diagnosis, environmental detection and so on. [0003] Flow cytometers with multiple excitation light sources and capable of analyzing various fluorescence parameters have always been a hot field for major flow cytometer manufacturers to compete for. At present, there are three main ways to improve the multi-color and multi-parameter measurement of flow cytometers. One is to develop new fluorescent probes, so that one wavelength laser can excite multiple fluorescent materials at the same time. Instrum...
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Inventor 高庆嘉赵建孙强
Owner CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI