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Coating method for seed balls of rhizoma-bletillae tissue culture seedlings

A technology of coating and seedlings, which is applied in the field of coating of white and tissue culture seedlings, can solve the problems of lack of nutrition, easy to be polluted by microorganisms, low survival rate of field sowing, etc., and achieve the effect of saving seed production land

Inactive Publication Date: 2015-04-08
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of nutrition and easy contamination by microorganisms during hardening, the survival rate of field sowing is very low.

Method used

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  • Coating method for seed balls of rhizoma-bletillae tissue culture seedlings
  • Coating method for seed balls of rhizoma-bletillae tissue culture seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Obtaining the white and tissue culture seed balls of coating comprises the following steps:

[0027] Step 1: Preparing white and white bulbs, obtaining a large number of white and white bulbs through tissue culture technology, and selecting bulbs with a size of 0.5g to 1.0g as bulbs for coating;

[0028] Step 2: Prepare nutrients, the composition of which is 1 / 2MS+2% sodium alginate+0.5mg / L NAA+0.1mg / L 6-BA+0.1mg / L IBA;

[0029] Step 3: prepare the seed coating agent, the composition of the seed coating agent is 2% sodium alginate+0.2mol / L CaCl 2 +0.5mg / L penicillin+0.1% sodium benzoate+0.5% carbendazim;

[0030] Step 4: mixing the nutrient substance prepared in step 2 with the seed coating agent prepared in step 3 to prepare mother liquor;

[0031] Step 5: Prepare the first layer of coating, put the bulbs obtained in step 1 into the mother liquor prepared in step 4 to form the first layer of coating, that is, the nutrient layer of bulbs;

[0032] Step 6: Prepare th...

Embodiment 2

[0034] Sow the successfully produced white and coated bulbs (test group) and uncoated white and coated bulbs (control group) in humus soil, grow at room temperature for 2 to 4 weeks, and observe the germination of the test group and the control group rate, and the results are shown in Table 1.

[0035]

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Abstract

The invention discloses a coating method for seed balls of rhizoma-bletillae tissue culture seedlings. The coating method comprises the following steps: preparing the rhizoma-bletillae seed balls; preparing nutrient substances; preparing a seed coating agent; preparing mother liquor by mixing the nutrient substances and the seed coating agent; preparing a first-layer coating; and preparing a second-layer coating. The coating has the characteristics of good air permeability, water retention, nutrition supplying, strong disease resistance and effect of promoting the growth of the rhizoma-bletillae seedlings, simultaneously also has the certain mechanical-damage-resistant action, and can be used for effectively protecting the seed balls in transportation and storage. The coating method disclosed by the invention is simple to operate, convenient to use and low in cost, and is a coating method capable of effectively increasing the rhizoma-bletillae emergence rate in the process of artificial rapid propagation of the rhizoma-bletillae seedlings.

Description

technical field [0001] The invention relates to a method for coating seed balls of bletilla seedlings, and belongs to the technical field of seedling cultivation of traditional Chinese medicinal materials. Background technique [0002] White and Bletilla striata (Thunb.) Reichb.f. is a perennial herb of Orchidaceae, and its medicinal parts are dry tubers. Baiji has the effects of nourishing the lungs, reducing swelling, promoting muscle growth, stopping bleeding, and suppressing sores, and has high medicinal value. In addition, Baiji has bright colors and is also a good landscape plant, so there is a large demand for Baiji in the market. Over-harvesting of wild basil in recent years has resulted in a sharp decrease in wild resources. In order to protect the wild resources of basil, artificial breeding and cultivation of basil must be adopted. [0003] The seeds of Baiji are very small and have no endosperm, so it is difficult to germinate and grow under natural conditions...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 黄明进刘剑东李丽颖赵致张明生
Owner GUIZHOU UNIV