A bovine embryo vitrification freezing tube thawing and direct transplantation method

A technology for vitrification and freezing of bovine embryos, which is used in medical science, preservation of human or animal bodies, and animal delivery, etc., can solve the problems of high production equipment requirements, unfavorable long-term storage, low freezing efficiency, etc. The tube is easy to operate and the effect of simple operation
CN104488853BActive Publication Date: 2016-08-24CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2016-08-24

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Abstract

The invention provides a bovine embryo vitrification tube swinging thawing and direct transplanting method and belongs to the technical field of embryo low-temperature biology. The method includes (1) freezing a bovine embryo and using a fine tube to first add a thawing liquid followed by air; (2) loading a freezing liquid followed by air; (3) loading a freezing liquid, placing the bovine embryo in the freezing liquid, and then loading air; (4) loading a thawing liquid, closing a tube opening, and performing freezing; (5) performing tube swinging thawing and direct transplanting, wherein the freezing liquid is an mPBS solution containing ethylene glycol (EG), polysucrose and sucrose, and the thawing liquid is an mPBS solution containing fetal bovine serum (FBS). According to the bovine embryo vitrification tube swinging thawing and direct transplanting method, the operation is simple and convenient, requirements for skills of operators are not high, the frozen bovine embryo can be directly transplanted after tube swinging thawing, the thawed embryo has a survival rate of 100% and an expansion rate of 94%, a pregnancy rate reaches 57% after transplantation, and the method is efficient, simple, easy to operate, and applicable to industrialized popularization.
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Description

Technical field

[0001] The invention relates to the technical field of animal embryo cryopreservation and embryo transfer, in particular to a method for thawing and direct transfer of a bovine embryo vitrified freezing tube. Background technique

[0002] Since Rall et al. (1985) invented the embryo vitrification cryopreservation method, the freezing process can be performed at room temperature without the aid of a freezer, and the procedure has been greatly simplified. After improvement by many researchers, the current vitrification method of oocytes / embryos only takes about 1 minute to complete a procedure. In the process, many researchers creatively invented many frozen load-bearing objects and named them various methods. The following methods are commonly used today: Cryoloop, Open Pulled Straw (OPS), GMP, Microdrops, semi-thin tube Law (hemi-straw). However, there are still many problems in the application of the above methods. For example, the number of oocytes / embryos fr...

Claims

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