A tumor-specific growth factor detection kit

A detection kit, tumor-specific technology, applied in the direction of chemical reaction of materials, material analysis by observing the influence of chemical indicators, etc., can solve the problem of high temperature accuracy requirements, unreliable test results, random Large error and other problems, to achieve the effect of high degree of automation, short test time and easy operation

Active Publication Date: 2017-01-25
HUNAN NEWLAND BIOTECH
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the continuous increase in the use of large body tests in the market, the shortcomings of the kit’s use and operation are gradually exposed: 1) The reagent contains hydrochloric acid and acetic acid, which is irritating to a certain extent and has an impact on the environment and operators; 2) Water bath High temperature accuracy requirements, 100°C±1°C, altitude changes and differences in heaters will cause temperature fluctuations, the fluctuation range is likely to exceed 1°C, resulting in unreliable test results; 3) Manual sample addition, manual colorimetry, Not only is the operation cumbersome, but also the random error is large

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Preparation of R1 reagent: Weigh the following components

[0087] Sodium dihydrogen phosphate 3.00g

[0088] Disodium edetate 0.59g

[0089] Kayson 0.30g

[0090] 4-Hydroxyethylpiperazine propanesulfonic acid 0.10g

[0091] Dissolve in purified water and prepare a 100mL solution, which is the R1 reagent;

[0092] Preparation of R2 reagent: Weigh the following components

[0093] Ninhydrin 0.08g

[0094] Kayson 0.50g

[0095] Sodium citrate 2.50g

[0096] Glycerin 1.00g

[0097] Dissolve in purified water and prepare a 100mL solution, which is the R2 reagent;

[0098] Preparation of standard solution:

[0099] Preparation of liquid A (standard solution with equivalent concentration of 0 U / mL): purified water;

[0100] Preparation of liquid B (standard solution with an equivalent concentration of 200U / mL): weigh the following components

[0101] Sialic acid 0.10g

[0102] Homocysteine ​​0.03g

[0103] Fructosamine 0.065g

[0104] Dissolve in purified water ...

Embodiment 2

[0106] Preparation of R1 reagent: Weigh the following components

[0107] Sodium dihydrogen phosphate 3.10g

[0108] Disodium edetate 0.61g

[0109] Kayson 0.50g

[0110] 4-Hydroxyethylpiperazine propanesulfonic acid 0.02g

[0111] Dissolve in purified water and prepare a 100mL solution, which is the R1 reagent;

[0112] Preparation of R2 reagent: Weigh the following components

[0113] Ninhydrin 0.25g

[0114] Kayson 0.30g

[0115] Sodium citrate 3.50g

[0116] Glycerin 4.50g

[0117] Dissolve in purified water and prepare a 100mL solution, which is the R2 reagent;

[0118] Preparation of standard solution:

[0119] Preparation of liquid A (standard solution with equivalent concentration of 0 U / mL): purified water;

[0120] Preparation of liquid B (standard solution with an equivalent concentration of 200U / mL): weigh the following components

[0121] Sialic acid 0.15g

[0122] Homocysteine ​​0.01g

[0123] Fructosamine 0.08g

[0124] Dissolve in purified water t...

Embodiment 3

[0126] Preparation of R1 reagent: Weigh the following components

[0127] Sodium dihydrogen phosphate 3.20g

[0128] Disodium edetate 0.60g

[0129] Kayson 0.10g

[0130] 4-Hydroxyethylpiperazine propanesulfonic acid 0.06g

[0131] Dissolve in purified water and prepare a 100mL solution, which is the R1 reagent;

[0132] Preparation of R2 reagent: Weigh the following components

[0133] Ninhydrin 0.16g

[0134] Kayson 0.10g

[0135] Sodium citrate 1.50g

[0136] Glycerin 8.00g

[0137] Dissolve in purified water and prepare a 100mL solution, which is the R2 reagent;

[0138] Preparation of standard solution:

[0139] Preparation of liquid A (standard solution with equivalent concentration of 0 U / mL): purified water;

[0140] Preparation of liquid B (standard solution with an equivalent concentration of 200U / mL): weigh the following components

[0141] Sialic acid 0.20g

[0142] Homocysteine ​​0.02g

[0143] Fructosamine 0.05g

[0144] Dissolve in purified water t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
absorbanceaaaaaaaaaa
Login to view more

Abstract

The invention relates to a tumor specific growth factor detection kit. The kit comprises an R1 reagent, an R2 reagent and a standard substance solution, the R1 reagent comprises phosphate, disodium ethylene diamine tetraacetate, Kathon and 4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid, and the solvent of the R1 reagent is purified water; the R2 reagent comprises a developer, Kathon, sodium citrate and glycerin, and the solvent of the R2 reagent is purified water; and the standard substance solution comprises a solution A and a solution B, the solution A is purified water, and the solution B is a sialic acid, homocysteine and fructosamine mixed solution. The kit has the advantages of substantial simplification of detection steps, cost saving, simple practical operation, no need of a precious device, and extremely easy popularization.

Description

technical field [0001] The invention relates to a kit, in particular to a tumor-specific growth factor detection kit. Background technique [0002] Since carbohydrates can form glycolipids, glycoproteins, oligosaccharides, etc., and are widely distributed inside and outside cells and in various body fluids, when cells become cancerous, their metabolic disorders can cause the content of body fluids to increase, which is an internationally recognized tumor marker. substances; amino acids and their metabolites are also suitable for general screening because of their small tumor specificity. After long-term research, among these several small molecule tumor markers, several selected markers are combined together and collectively called TSGF (Tumor Specific Growth of Factor, tumor-specific growth factor). When a tumor occurs, the content of tumor-specific growth factors in the blood increases, which is an important clue for the early detection, postoperative, radiotherapy, chemo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 宋光运林惠高冬林芳
Owner HUNAN NEWLAND BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap