A tumor-specific growth factor detection kit

Abstract

The invention relates to a tumor specific growth factor detection kit. The kit comprises an R1 reagent, an R2 reagent and a standard substance solution, the R1 reagent comprises phosphate, disodium ethylene diamine tetraacetate, Kathon and 4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid, and the solvent of the R1 reagent is purified water; the R2 reagent comprises a developer, Kathon, sodium citrate and glycerin, and the solvent of the R2 reagent is purified water; and the standard substance solution comprises a solution A and a solution B, the solution A is purified water, and the solution B is a sialic acid, homocysteine and fructosamine mixed solution. The kit has the advantages of substantial simplification of detection steps, cost saving, simple practical operation, no need of a precious device, and extremely easy popularization.

Description

technical field [0001] The invention relates to a kit, in particular to a tumor-specific growth factor detection kit. Background technique [0002] Since carbohydrates can form glycolipids, glycoproteins, oligosaccharides, etc., and are widely distributed inside and outside cells and in various body fluids, when cells become cancerous, their metabolic disorders can cause the content of body fluids to increase, which is an internationally recognized tumor marker. substances; amino acids and their metabolites are also suitable for general screening because of their small tumor specificity. After long-term research, among these several small molecule tumor markers, several selected markers are combined together and collectively called TSGF (Tumor Specific Growth of Factor, tumor-specific growth factor). When a tumor occurs, the content of tumor-specific growth factors in the blood increases, which is an important clue for the early detection, postoperative, radiotherapy, chemo...

AI Technical Summary

Problems solved by technology

With the continuous increase in the use of large body tests in the market, the shortcomings of the kit’s use and operation are gradually exposed: 1) The reagent contains hydrochloric acid and acetic acid, which is irritating to a certain extent and has an impact on the environment and operators; 2) Water bath High temperature accuracy requirements, 100°C±1°C, altitude changes and differences in heaters will cause temperature fluctuations, the fluctuation range is likely to exceed 1°C, resulting in unreliable test results; 3) Manual sample addition, manual colorimetry, Not only is the operation cumbersome, but also the random error is large