A New Breeding Method of Indica-Japonica Cross Gene Introgression for Resistance to Rice Blast

A technology of rice blast resistance and gene introgression, applied in the field of agricultural science, can solve the problems of indica and japonica crossing, difficult for breeders to master, and high technical threshold, so as to reduce screening workload, shorten breeding cycle, and low operating threshold Effect

Active Publication Date: 2017-03-29
YIELEAD RICE IND
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Problems solved by technology

However, the transgenic breeding of rice blast resistance gene and other excellent genes for specific elite main varieties could have accurately achieved the improvement of indica and japonica crosses. It also requires multi-generation hybrid backcrossing in the field, which cannot solve the problem of indica-japonica crossing. In addition, molecular technology breeding has the problems of high cost and high technical threshold, and it is difficult for ordinary breeders to master

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  • A New Breeding Method of Indica-Japonica Cross Gene Introgression for Resistance to Rice Blast

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Embodiment

[0050] (1) Screening S0412 and Digu as japonica breeding parents and indica rice blast resistance sources respectively:

[0051] Years of breeding practice have found that the main breeding line S0412 has the characteristics of high yield and excellent rice quality. At the same time, the average plant height of S0412 is only 92.8cm in two years, and the whole growth period is only 142 days. The anthers of S0412 were inoculated on the indica medium for induction culture. The experiment found that the callus induction rate of S0412 was as high as 54% (the anther culture procedure was the same as before), which is very suitable for the requirements of the japonica rice breeding parent of this technical route. Enhui 58 is an indica-type conventional rice hybrid bred by Hui 63 as the female parent and Miryang 46 as the male parent by the Academy of Agricultural Sciences in Enshi Autonomous Prefecture, Hubei Province. It is one of the important sources of resistance to rice blast re...

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Abstract

The invention discloses a new breeding method for indica-japonica hybrid gene introgression rice resistant to rice blast. The new breeding method comprises the following steps of 1 selecting a japonica rice variety / series to be improved and an indica rice resistant source resistant to rice blast, 2 carrying out course identification of toxin resistant concentration on the selected japonica rice variety / series, 3 carrying out cross breeding to obtain three-way cross F1 hybrid cultivar, 4 carrying out indica removing culture medium anther culture on the three-way cross F1 hybrid cultivar, 5 planting anther cultured seedlings in fields, harvesting single plants to obtain a plurality of japonica rice anther cultured families, further sowing the different japonica rice anther cultured families, and obtaining a japonica rice new strain with good agronomic characters and improved rice blast resistance by using the parents of the japonica rice variety / series to be improved as comparison. According to the new breeding method for indica-japonica hybrid gene introgression rice resistant to rice blast, rice blast resistant genes of indica rice can be specifically and efficiently infiltrated into the japonica rice, rice blast resistant seedling can be completed in about three years, the conventional seedling period of indica-japonica hybrid rice resistant to rice blast is shortened obviously, and the method has large breeding application value.

Description

technical field [0001] The invention relates to the field of agricultural science and technology, in particular to a rice breeding method for specifically and efficiently infiltrating an indica rice blast resistance gene into japonica rice, specifically a new method for introgression rice blast resistance breeding of an indica-japonica cross gene. Background technique [0002] Rice is one of the most important food crops in the world, its cultivated area and total output are second only to wheat. Rice production is particularly important to developing countries, as four-fifths of Asia's two billion people and one-third of Africa's and Latin America's nearly one billion people depend on rice as their main food source. my country is a big country of rice production. According to statistics in the past 10 years, the average annual rice production accounts for 36.9% of the world's total rice production, ranking first in the world; the rice sown area accounts for 22.8% of the wo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01H1/02
Inventor 乔保建邓建社夏祥华季成文郑立平付锡江程驭
Owner YIELEAD RICE IND
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