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A new breeding method for resistance to sheath blight by introgression of indica and japonica genes

A technology of gene introgression and sheath blight, applied in the field of agricultural science, can solve the problems of indica and japonica crossing, difficult for breeders to master, high technical threshold, etc., to reduce screening workload, shorten breeding cycle, and low operating threshold Effect

Inactive Publication Date: 2017-06-20
兰进好
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the transgenic breeding of the sheath blight resistance gene and other superior genes for specific elite main cultivars could have accurately achieved the improvement of indica and japonica crosses. , which also requires multi-generation hybrid backcrossing in the field, which cannot solve the problem of indica and japonica crossing. In addition, molecular technology breeding has the problems of high cost and high technical threshold, and it is difficult for ordinary breeders to master

Method used

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  • A new breeding method for resistance to sheath blight by introgression of indica and japonica genes
  • A new breeding method for resistance to sheath blight by introgression of indica and japonica genes
  • A new breeding method for resistance to sheath blight by introgression of indica and japonica genes

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Embodiment

[0049] (1) Screening W09557 and Teqing respectively as japonica rice breeding parents and indica rice sheath blight resistance sources:

[0050] Years of breeding practice have found that the main breeding line W0955 has the characteristics of high yield and relatively good rice quality. At the same time, the average plant height of W0955 measured in two years is only 92.8cm, and the whole growth period is only 146 days. It is susceptible to sheath blight. In 2011 The anthers of W0955 were inoculated in the indica rice medium for induction culture in the positive season. The experiment found that the callus induction rate of W0955 was as high as 48% (the anther culture procedure is the same as before), which is very suitable for the requirements of the japonica rice breeding parent of this technical route. Teqing is highly resistant to multiple sheath blight strains in many areas of sheath blight disease, and is one of the important antigens for sheath blight resistance breedin...

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Abstract

The invention discloses a new breeding method for introgression resistance to sheath blight of indica and japonica genes. The steps of the new breeding method are as follows: (1) selecting the japonica rice variety / line to be improved and the indica rice resistance source for sheath blight resistance; (2) Identification of crude toxin tolerance concentration for the selected japonica rice varieties / lines to be improved; (3) hybrid breeding to obtain three-cross F1 hybrids; (4) anther culture for three-cross F1 hybrids; (5) field Plant flower seedlings, and harvest a single plant to obtain several japonica rice flower culture lines with improved sheath blight resistance. Different flower culture families are further sown in the field, and the japonica rice varieties / line parents to be improved are used as controls to screen and obtain good agronomic shape and sheath blight. A new japonica rice line with improved disease resistance. The new breeding method of the present invention can specifically and efficiently infiltrate the indica rice sheath blight resistance gene into japonica rice, and the sheath blight resistance breeding can be completed in about 3 years, which significantly shortens the conventional indica-japonica cross resistance sheath blight breeding cycle, and has a greater Application value of breeding.

Description

technical field [0001] The invention relates to the field of agricultural science and technology, in particular to a rice breeding method for specifically and efficiently infiltrating an indica rice sheath blight resistance gene into japonica rice, specifically a new indica-japonica cross gene introgression resistance to sheath blight breeding method. Background technique [0002] Rice sheath blight (rice sheath blight), commonly known as rotten feet, flower stalks, and foot blight, is one of the important diseases in rice production and is a disease of rice all over the world. The disease was first discovered in Japan in 1905, and was reported in my country in 1934, and then spread rapidly and spread to Africa, Europe and the Americas. At present, the disease has generally occurred in rice-growing countries in Asia, Africa, Europe, and the United States. It generally reduces production by 10% to 30%, and can reach 50% in severe cases. It poses a great threat to rice produc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H4/00
Inventor 兰进好
Owner 兰进好
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