Rapid vitrification preservation and frozen refrigeration method for little yellow croakers and special refrigerating fluid
A freezing liquid and vitrification technology, applied in the direction of freezing/cooling preservation of meat/fish, etc., can solve the problems of death, cell damage, cell osmotic pressure damage, etc., and achieve the effect of simple preparation method, simple raw materials and easy access to raw materials
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Embodiment 1
[0016] The composition and weight percentage of the freezing liquid are: 13% of sodium chloride, 18% of ethanol, 19% of glycerin, 5% of edible sucrose, 0.5% of sodium tripolyphosphate, and the balance of water.
[0017] At -29°C, put the small yellow croaker that needs to be frozen and kept fresh in the freezing liquid for 5 minutes, then wash it with zero-degree ice water and wear ice clothes, and then put it in a freezer at minus 18°C for cold storage.
[0018] Take vitrified fresh-preserved frozen small yellow croaker, common frozen small yellow croaker, and fresh small yellow croaker muscle tissue on both sides of the back, cut into small pieces of 8mm×8mm×8mm, and test the frozen samples after natural thawing at room temperature, and cut the fresh yellow croaker directly for testing. The American TMS-PRO texture analyzer is used for detection (P / 0.5 cylindrical probe is selected, the test speed is 60mm / min, the deformation amount is 35%, and the interval between two pres...
Embodiment 2
[0023] The difference from Example 1 is that the composition and weight percentage of the freezing liquid used are: 13% sodium chloride, 10% ethanol, 19% glycerin, 3% edible sucrose, 0.2% sodium tripolyphosphate, and the water balance. The test results of the obtained frozen fresh-keeping products are shown in Table 1.
Embodiment 3
[0025] The difference from Example 1 is that the composition and weight percentage of the freezing liquid used are: 12% sodium chloride, 17% ethanol, 20% glycerin, 5% edible sucrose, 0.5% sodium tripolyphosphate, and the water balance. Place the small yellow croaker in freezing liquid and freeze for 10 minutes at -25°C.
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