Method for extracting carnosol from complete salvia
A technology of carnosol and sage, which is applied in the field of extracting natural active compounds, can solve the problems of low quality and yield, and can not effectively preserve the active ingredients of sage, so as to change the low added value, quality and Increased yield and good social benefits
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Embodiment 1
[0008] S1: cleaning, disinfection, and rinsing of the whole sage grass: washing in clean water, and disinfecting with 3% potassium permanganate solution, and then rinsing in clean water;
[0009] S2: Grinding and extracting the juice of sage: the slurry is ground to a thickness of 200 meshes;
[0010] S3: rough extraction of functional components: NaOH is added to the 5% aqueous ethanol solution, the pH value of the adjusted aqueous ethanol solution is 9.5, and the weight ratio of the aqueous ethanol solution and sage is 3:1, and extracted at 90° C. for 3 hours;
[0011] S4: organic solvent extraction: the crude extract is added to the polar solvent with the pH value of 5 times of weight and is extracted, and extracted at normal temperature for 10 hours;
[0012] S5: separation and purification: the extract is separated by membrane separation, column chromatography, and the detection of the functional component content and the yield of the extract is detected;
[0013] S6: va...
Embodiment 2
[0016] S1: cleaning, disinfection, and rinsing of the whole sage grass: washing in clean water, and disinfecting with 3% potassium permanganate solution, and then rinsing in clean water;
[0017] S2: Grinding and extracting the juice of sage: the slurry is ground to a thickness of 200 meshes;
[0018] S3: crude extraction of functional components: NaOH is added to the 5% aqueous ethanol solution, the pH value of the adjusted aqueous ethanol solution is 9.5, and the weight ratio of the aqueous ethanol solution and sage is 3:1, and extracted at 90° C. for 3 hours;
[0019] S4: organic solvent extraction: the crude extract is added in the polar solvent extraction with the pH value of 5 times of weights, and is extracted at normal temperature for 10 hours;
[0020] S5: separation and purification: the extract is separated by membrane separation, column chromatography, and the detection of the functional component content and the yield of the extract is detected;
[0021] S6: vacu...
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