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Method for extracting high-purity persimmon tannin from persimmon fruits

A high-purity, persimmon tannin technology, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve problems such as low purity of persimmon tannin, and achieve strong operability, low analysis cost, and high performance. stable effect

Inactive Publication Date: 2015-04-22
桂林得坤生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the prepared persimmon tannin has low purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Wash the immature fresh persimmons with a green color and astringent taste, and then crush them for later use;

[0022] (2) add volume concentration and be 7% ethanol reflux extraction 3 times, the first addition of ethanol is 12 times of persimmon volume, the second addition of ethanol is 10 times of persimmon volume, the third addition of ethanol is persimmon volume 9 times the volume, each extraction time is 150min, and the extraction temperature is 85°C each time, the extracts are combined, evaporated to dryness, and the crude persimmon tannin is obtained;

[0023] (3) Mix n-hexane, ethyl acetate, methanol, and water in a volume ratio of 5:1:1:6, shake well, let stand for stratification, and after equilibrium stratification, the solution is divided into upper and lower phases, and the lower phase is used as the stationary phase , the upper phase is used as the mobile phase;

[0024] (4) The persimmon tannin crude product obtained in step (3) is dissolved in a m...

Embodiment 2

[0028] (1) Wash the immature fresh persimmons with a green color and astringent taste, and then crush them for later use;

[0029] (2) adding volume concentration is 75% ethanol backflow extraction 3 times, the first addition of ethanol is 11 times of persimmon volume, the second addition of ethanol is 9 times of persimmon volume, the third addition of ethanol is persimmon 8 times the volume, each extraction time is 140min, each extraction temperature is 90°C, the extracts are combined, evaporated to dryness, and the crude persimmon tannin is obtained;

[0030] (3) Mix n-hexane, ethyl acetate, methanol, and water in a volume ratio of 5:1:1:6, shake well, let stand for stratification, and after equilibrium stratification, the solution is divided into upper and lower phases, and the lower phase is used as the stationary phase , the upper phase is used as the mobile phase;

[0031] (4) dissolving the persimmon tannin crude product obtained in step (3) in a mobile phase of 16 tim...

Embodiment 3

[0035] (1) Wash the immature fresh persimmons with a green color and astringent taste, and then crush them for later use;

[0036] (2) add volume concentration and be 80% ethanol reflux extraction 3 times, the first addition of ethanol is 11 times of persimmon volume, the second addition of ethanol is 9 times of persimmon volume, the third addition of ethanol is persimmon 7 times the volume, each extraction time is 125min, each extraction temperature is 90°C, the extracts are combined, evaporated to dryness, and the crude persimmon tannin is obtained;

[0037] (3) Mix n-hexane, ethyl acetate, methanol, and water in a volume ratio of 5:1:1:6, shake well, let stand for stratification, and after equilibrium stratification, the solution is divided into upper and lower phases, and the lower phase is used as the stationary phase , the upper phase is used as the mobile phase;

[0038] (4) The persimmon tannin crude product obtained in step (3) is dissolved in a mobile phase of 18 ti...

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Abstract

The invention belongs to the technical field of preparation of persimmon tannin and particularly relates to a method for extracting high-purity persimmon tannin from persimmon fruits. The method comprises the following steps of: washing the persimmon fruits, smashing, extracting with ethanol in a refluxing way, carrying out liquid-liquid chromatographic separation and purification on a high-speed countercurrent chromatographic instrument by using a two-phase solvent system to obtain high-purity persimmon tannin, wherein the two-phase solvent system is composed of n-hexane, ethyl acetate, methanol and water at a volume ratio of 5:1:1:6. The process provided by the invention is simple, is easy to operate and is low in analysis cost, and the prepared persimmon tannin is high in purity.

Description

technical field [0001] The invention belongs to the technical field of persimmon tannin preparation, in particular to a method for extracting high-purity persimmon tannin from persimmon. Background technique [0002] Persimmon, a berry fruit of the persimmon family, originated in China and has been cultivated for more than one thousand years. my country is the country of origin of persimmons, and it is also the country with the largest persimmon cultivation area and the largest persimmon fruit output in the world. Whether it is cultivation area or fruit yield, my country ranks first in the world, much larger than the sum of other countries in the world. Traditional Chinese medicine believes that persimmon is sweet, astringent, cold in nature, and belongs to the lung meridian. "Compendium of Materia Medica" records that "persimmon is the fruit of the spleen, lungs, and blood. It is sweet in taste and flat in Qi, astringent in nature and can be harvested, so it has the effec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H13/08C07H1/08
Inventor 刘小峰何明茜罗知颂李京生刘二伟
Owner 桂林得坤生物科技股份有限公司