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Hepatocarcinoma marker melanoma cell-adhesion molecule and application thereof

A technology for melanoma and liver cancer, which is applied in the field of markers for the diagnosis of liver cancer and its application, and can solve the problems of increased negative ratio of AFP, unsatisfactory sensitivity and specificity, etc.

Inactive Publication Date: 2015-04-22
HANGZHOU JOINSTAR BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although alpha-fetoprotein plays a positive role in the diagnosis of liver cancer, its sensitivity and specificity are not satisfactory, and the proportion of AFP-negative cases in new cases is increasing

Method used

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  • Hepatocarcinoma marker melanoma cell-adhesion molecule and application thereof
  • Hepatocarcinoma marker melanoma cell-adhesion molecule and application thereof
  • Hepatocarcinoma marker melanoma cell-adhesion molecule and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0156] Detection of MCAM expression in human liver cancer and normal liver tissues by tissue microarray (TMA) analysis

[0157] 418 liver cancer tissue samples and 24 normal liver tissue samples were stained and analyzed by tissue microarray (TMA). Such as Figure 3A As shown, 304 cases of immunohistochemical membrane staining were positive in 418 cases of liver cancer tissues, the ratio was about 72.7%. However, none of the 24 cases of normal liver tissue stained positively.

[0158] Data analysis showed that MCAM protein expression was significantly stronger in membrane staining in liver cancer tissues than in normal liver tissues.

Embodiment 2

[0160] Immunohistochemical Analysis of MCAM Expression in Human Colon and Breast Cancer Tissues

[0161] IHC staining and analysis were performed on 5 normal and 24 colon cancer tissue samples, and the staining of the corresponding tissue cells was counted. Such as Figure 3G As shown, no statistical difference was found between normal tissue and colorectal cancer tissue samples (p<0.01). In addition, it can be seen that MCAM is mostly located on the surface of acini or blood vessels, and rarely seen on the surface of colon tissue cells.

[0162] Similarly, 5 cases of normal and 24 cases of breast cancer tissue samples were stained and analyzed by IHC, and the staining conditions of corresponding tissue cells were counted. Such as Figure 3H As shown, no statistical difference was found between normal tissue and breast cancer tissue samples (p<0.01). In addition, it can be seen that MCAM is mostly located on the surface of acini or blood vessels, and rarely seen on the sur...

Embodiment 3

[0165] A group of liver cancer cell lines SMMC-7721, Bel-7402 and Huh7 were selected to observe the membrane localization of MCAM by immunofluorescence. Such as Figure 1B As shown, it can be seen that MCAM is clearly located on the cell membrane.

[0166] Data analysis revealed that MCAM protein is a liver cancer-specific marker located on the cell membrane.

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Abstract

The invention relates to a hepatocarcinoma marker and its application. Specifically, the applicant identifies melanoma cell-adhesion molecule (MCAM) as a novel serum tumor marker of YAP dependent liver cancer. Experimental results of the applicant simultaneously prove that MCAM activity is crucial to hepatoma cell survival and transformation and show that the MCAM can be applied as a potential direct anticancer drug target. The invention provides an application of gene, mRNA or cDNA of the MCAM as a hepatocarcinoma detection marker, or in the preparation of a reagent or a kit for detection of liver cancer. Researches show that the amount of MCAM in serum of patients with liver cancer is remarkably higher than that of MCAM in serum of normal persons as well as serum of patients with other hepatic diseases and non-hepatoma diseases. Data analysis shows that MCAM protein is a quantitative sensitive hepatoma-specific serum marker.

Description

technical field [0001] The present invention relates to the fields of oncology and diagnostics. More specifically, the present invention relates to a marker for diagnosing liver cancer and its use. Background technique [0002] Liver cancer is a common malignant tumor in China, ranking third in tumor incidence and second in mortality. Primary hepatocellular carcinoma (hepatocellular carcinoma, HCC) is the most common type of liver cancer. [0003] Liver cancer patients in China account for 54% of the world's incidence, and men are more likely to develop the disease than women. Currently, the 5-year survival rate of liver cancer patients is less than 5%. About 549,000 patients die from it every year in the world, and the incidence rate is increasing year by year (from the WHO mortality database). Therefore, it is of great significance to increase the prevention and treatment of liver cancer to reduce the mortality rate. [0004] At present, the diagnosis of liver cancer ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/68G01N33/577G01N33/573
CPCC12Q1/68G01N33/577G01N33/68C12Q1/6886A61K45/00C12Q2600/158G01N33/57438G01N2333/47
Inventor 孙韶周何博汇孙晋华周旭一
Owner HANGZHOU JOINSTAR BIOTECH
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