Tissue culture and propagation method of viburnum tinus

A technology of Mediterranean viburnum and culture medium, which is applied in the field of agronomy, can solve the problems of the undisclosed rapid tissue culture and propagation method of Mediterranean viburnum, and achieve the effects of saving manpower and material resources, simple equipment, and less floor space

Inactive Publication Date: 2015-04-29
CHONGQING UNIV OF ARTS & SCI
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] But the fast tissue culture propagation method of effective Viburnum mediterranei has not yet been disclosed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue culture and propagation method of viburnum tinus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] The present invention will be described in detail below in conjunction with specific embodiments. It should be noted that the technical features or combinations of technical features described in the following embodiments should not be regarded as isolated, and they can be combined with each other to achieve better technical effects.

[0014] figure 1 Shown is a flow chart of a method for tissue culture propagation of Viburnum mediterranei according to an embodiment of the present invention.

[0015] The tissue culture propagation method of Viburnum mediterranei provided by the embodiments of the present invention comprises the following steps:

[0016] Step 101: Selection and treatment of explants: Select the stem section of Viburnum mediterranei with axillary buds as the culture material, rinse with running water; sterilize with alcohol on the ultra-clean bench, rinse with sterile water, and then sterilize with mercuric chloride , then rinsed with sterile water, and...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a tissue culture and propagation method of viburnum tinus. The tissue culture and propagation method comprises the following steps: selection and treatment of explants: selecting stem segments with axillary buds of viburnum tinus as culture materials, rinsing with water, performing surface sterilization with alcohol on a super clean bench, rinsing with sterile water, sterilizing with mercuric chloride, rinsing with sterile water, and inoculating in an induction medium; induction culture: taking an MS medium to be mixed with 6-benzylamino adenine, naphthylacetic acid, sucrose and agar, using the mixture I as the induction medium, and inoculating the selected stem segments with axillary buds of viburnum tinus in the induction medium; proliferation culture: transferring the explants growing in the induction medium to the induction medium formed by mixing the MS medium with 6-benzylamino adenine, naphthylacetic acid, sucrose and agar, and performing continuous culture; rooting culture: selecting 1 / 2 MS medium to be mixed with indolebutyric acid, activated carbon, sucrose and agar, and using the mixtureII as a rooting medium; transplanting: taking out the rooted plants, and transplanting into a mixed medium containing humus soil, cinders and sawdust.

Description

technical field [0001] The invention relates to the technical field of agronomy, and more specifically relates to a method for tissue culture propagation of Mediterranean viburnum (Viburnum tinus). Background technique [0002] The crown of the Mediterranean viburnum is spherical, and the crown diameter can reach 2.5 to 3 meters. The leaves are oval, dark green, 10 cm long, cyme, single flower is small, only 0.6 cm, the flower buds are pink, the flower bud period is very long, up to more than 5 months, the flowers are white after blooming, and the diameter of the whole inflorescence reaches 10 cm, the flowering period is from November to April in the next spring in the place of origin. Tiny yellow-green flower buds can be seen in early October in the Shanghai area. As the inflorescences elongate, the flower buds become more and more densely covered on the top of the branches, and the color gradually deepens to bright red. Looking from a distance, they look like pieces of re...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王大平
Owner CHONGQING UNIV OF ARTS & SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap