Method for extracting and separating 6,8-dihydroxy-elimofin-7(11)-ene-12,8-lactone from cucurbit seven and its application
A technology of dihydroxyl and cucurbit seven, which is applied in non-central analgesics, antipyretics, organic chemistry, etc., can solve problems that have not been seen, and achieve the effects of simple operation, mild extraction conditions, and simple equipment
Inactive Publication Date: 2016-08-24
XI AN JIAOTONG UNIV
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Problems solved by technology
6,8-dihydroxy-erimorphen-7(11)-en-12,8-lactone belongs to sesquiterpenoids, and it has been reported that it can be obtained from plants of the genus Rhubarb and Muli. The compound was isolated, but there was no research report on obtaining the compound from Cucurbit Seven, nor was there any anti- Research reports on inflammatory activity
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Embodiment 1
[0034] Preparation of 6,8-dihydroxy-erimofen-7(11)-ene-12,8-lactone
[0035] Take 4kg of dry root and rhizome coarse powder of calabash odorifera and extract three times by cold soaking with 95% ethanol. The volume (L) added for the three times is 6, 4 and the mass (kg) of calabash odorifera root and rhizome coarse powder respectively. 4 times, cold soaking for three days each time, stirring three times a day, 10 minutes each time. After filtering, discarding the medicine residue, combining the extracts, and recovering the solvent to obtain 433.0 g of a brown ethanol extract.
[0036] After mixing the obtained ethanol extract with diatomaceous earth, first use petroleum ether (60-90℃) Soxhlet extraction to remove fat, remove the strong lipophilic part, and then use dichloromethane as a solvent for Soxhlet extraction for 18 hours, and recover Dichloromethane solvent to obtain dichloromethane extract, a total of 160.6g.
[0037] Take 55.0g of the dichloromethane extract and perform c...
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Abstract
The invention discloses a method for extracting and separating 6,8-dihydroxy-eremophilen-7(11)-ene-12,8-olide from cucurbit 7 and application thereof, belonging to the technical field of natural product chemistry. The method comprises the following steps: 1) taking cucurbit 7 medicinal material coarse powder, and extracting by carrying out cold soaking in 95% ethanol to obtain an ethanol extract; 2) degreasing the ethanol extract by petroleum ether (60-90 DEG C) Soxhlet extraction, carrying out Soxhlet extraction with dichloromethane, and recovering the solvent to obtain the dichloromethane extract; and 3) carrying out silica gel column chromatographic separation on the dichloromethane extract, collecting the petroleum ether (60-90 DEG C)-ethyl acetate (6:1) eluate, standing to obtain a brown yellow precipitate, refining to obtain a white irregular prism crystal which is the 6,8-dihydroxy-eremophilen-7(11)-ene-12,8-olide by structural identification. The invention also discloses anti-inflammatory action of the 6,8-dihydroxy-eremophilen-7(11)-ene-12,8-olide. Thus, the 6,8-dihydroxy-eremophilen-7(11)-ene-12,8-olide can be used for preparing anti-inflammatory drugs.
Description
Technical field [0001] The invention belongs to the technical field of natural product chemistry, and specifically relates to a method for extracting and separating 6,8-dihydroxy-erimofen-7(11)-ene-12,8-lactone from fennel and its application. Background technique [0002] Anti-inflammatory drugs are the second largest class of drugs after anti-infective drugs. Some active ingredients of traditional Chinese medicines such as glycosides, alkaloids, flavonoids, terpenoids, volatile oils, etc. have good anti-inflammatory effects, and the resources of traditional Chinese medicines are abundant. , Has a wide range of pharmacological effects, has anti-inflammatory effects, immune regulation, clearing heat and detoxification, promoting blood circulation and removing blood stasis and other pharmacological effects. At present, people pay more and more attention to the research of anti-inflammatory drugs in natural medicine. [0003] Gourd Qi, also known as Ligularia vulgaris, Dajiujia, Heye...
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IPC IPC(8): C07D307/92A61P29/00
CPCC07D307/92
Inventor 石娟田英超曹胜傅强孟歌贺改燕孙博杨硕鹏张唯佳赵丹
Owner XI AN JIAOTONG UNIV