Platelet-rich plasma separation gel and platelet-rich plasma preparation method

A technology of platelet-rich plasma and separation gel, which is applied in the field of platelet-rich plasma separation gel and the preparation of platelet-rich plasma using the separation gel, can solve the problems of preparation system pollution, easy inhalation of blood cells, incomplete recovery of platelets, etc. and easy dispersibility, prevent external contamination, and reduce the effect of application risk

Active Publication Date: 2015-04-29
南雄阳普医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] (1) After centrifugation and stratification, no effective isolation can be formed. Since the specific gravity of platelets is close to that of blood cells, most of the platelets will stop at the junction of plasma and blood cells. During the absorption process, it is easy to inhale blood cells, and it is easy to re-mix, resulting in failure to collect PRP;
[0012] (2) The open preparation system is susceptible to external contamination;
[0014] (4) T

Method used

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  • Platelet-rich plasma separation gel and platelet-rich plasma preparation method
  • Platelet-rich plasma separation gel and platelet-rich plasma preparation method
  • Platelet-rich plasma separation gel and platelet-rich plasma preparation method

Examples

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Example Embodiment

[0055] [Example 1]

[0056] Such as figure 2 The manufacturing method of the platelet-rich plasma separation gel of this embodiment is:

[0057] Step1: Synthesize basic polymer:

[0058]

[0059] The raw materials are mixed according to the above formula, placed in a sealed reactor, mixed, heated to 80-120°C, reacted for 6-8 hours to synthesize the basic compound.

[0060] Step2. High-temperature and high-vacuum distillation purification: Use water jet-Roots two-stage series vacuum pump to make the system vacuum lower than -0.098Mpa, and the temperature rises to 190-200 degrees for 6-8 hours to obtain relatively pure polymer polymerization Material, that is, resin.

[0061] Step3. Fine filtration: 250 mesh fine filtration to the mixing tank to remove impurities and accurately weigh the resin.

[0062] Step4, the specific gravity agent accurately adjusts the specific gravity: After the previous step, the weight of the basic polymer compound obtained is 600kg. Select 24 kg of nano-grade...

Example Embodiment

[0068] [Example 2]

[0069] In the platelet-rich plasma separation gel of this embodiment, the product formula of the basic polymer compound is:

[0070]

[0071] The weight ratio of the added silica to the base polymer and the weight ratio of BYK-420 organic thixotropic agent to the base polymer are 4.6:100 and 1.1:100, respectively. The preparation method is the same as that of Example 1, and it is prepared according to the above formula The obtained plasma separator gel has a measured specific gravity of 1.075, which can separate and enrich leukocytes and platelets, obtain leukocyte-rich platelet plasma (L-PRP), and increase the effect of anti-inflammatory treatment while repairing tissue.

Example Embodiment

[0072] [Example 3]

[0073] In the platelet-rich plasma separation gel of this embodiment, the product formula of the basic polymer compound is:

[0074]

[0075] The weight ratio of the added silica to the base polymer and the weight ratio of BYK-420 organic thixotropic agent to the base polymer are 5:100 and 1.2:100, respectively. The preparation method is the same as that of Example 1, and it is prepared according to the above formula. The obtained plasma separator gel has a measured specific gravity of 1.080, which can separate and enrich lymphocytes, monocytes and platelets to obtain mononuclear cell-rich platelet plasma (M-PRP), which is used for lymphocyte immune function detection, human leukocyte antigen ( HLA) or residual leukemia gene detection, and used for simple arteriosclerosis obliterans, thromboangiitis obliterans, diabetic lower limb ischemia and other diseases and treatments.

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Abstract

The invention discloses a platelet-rich plasma separation gel, a vacuum blood collection tube which is prepared by utilizing the separation gel and used for separating platelet-rich plasma, and a separation method. The platelet-rich plasma separation gel particularly comprises a resin, and the resin is prepared from the following raw materials in parts by weight: 120 parts of butyl acetate, 75-80 parts of phenethylene, 20-25 parts of butyl acrylate, 5-12 parts of acrylic acid, 2-2.5 parts of azodiisobutyronitrile (AIBN), and 0.5-0.8 parts of dodecyl mercaptan, and the specific weight ranges from 1.068 to 1.080 at the temperature of 25 DEG C and can be accurately adjusted according to the raw material ratio. The separation gel prepared by use of the resin and the manufactured vacuum blood collection tube are also included. When the blood collection tube is used for preparing platelet-rich plasma, the process is simple, convenient and easy, and the required time is short; repeated tube transfer is not required, so that cross contamination is avoided; and the operation is simple and convenient, the preparation cost is greatly reduced, the practicability and the feasibility are greatly improved, and a wide market prospect is achieved.

Description

technical field [0001] The invention belongs to the field of blood sampling and separation, and in particular relates to a platelet-rich plasma separation gel and a method for separating and preparing platelet-rich plasma by using the separation gel. Background technique [0002] Platelet-rich plasma (Platelet-rich plasma, PRP) is platelet-concentrated plasma obtained from autologous whole blood after centrifugation. Platelets contain a large number of growth factors, such as platelet-derived growth factor (PDGF), transforming growth factor-β (TGF-β), insulin-like growth factor (IGF), epidermal growth factor (EGF), vascular endothelial growth factor (VEGF) These growth factors are mainly secreted by platelet activation, which can promote the regeneration of cells and matrix in tissues from different aspects, thereby promoting tissue repair. [0003] Among the existing PRP preparation methods, according to the number of centrifugation, it can be divided into one-time, two-ti...

Claims

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Application Information

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IPC IPC(8): C08F212/08C08F220/18C08F220/06C08K3/36G01N1/34G01N33/48
Inventor 马海波汤习锋陈万能凌聪廖南山陈琼娣
Owner 南雄阳普医疗科技有限公司
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