Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

CHO (Chinese hamster ovary) cell culture technology capable of reducing content of acidic variants

A culture process and cell culture technology, which is applied in the field of cell culture, can solve the problems of charge heterogeneity, affecting the validity period, affecting antibody activity, etc., and achieve the effects of improving quality, easy operation, and increasing antibody expression

Active Publication Date: 2015-04-29
SUNSHINE LAKE PHARM CO LTD
View PDF1 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] During the production or storage of antibodies, due to the influence of complex culture environment, such as transcriptional modification or spontaneous degradation, the surface charges of antibodies are often different, resulting in charge heterogeneity, such as glutathionylation, deamidation and Sialylation produces acidic variants, and succinimidylation, amidation, and C-terminal lysine modifications produce basic variants that affect not only the activity of the antibody but also its shelf life

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • CHO (Chinese hamster ovary) cell culture technology capable of reducing content of acidic variants
  • CHO (Chinese hamster ovary) cell culture technology capable of reducing content of acidic variants
  • CHO (Chinese hamster ovary) cell culture technology capable of reducing content of acidic variants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Cell line A was used to express and culture recombinant human tumor necrosis factor receptor-Fc recombinant protein (rhTNFR-Fc) antibody

[0040] Table 1, Table 2, figure 1 and figure 2Cell line A-CD FortiCHO, Cell line A-TFS-RDMP-1, Cell line A-TFS-RDMP-9 and Cell line A-Hycell CHO refer to CD FortiCHO basal medium with 50% of the total volume of basal medium For inoculation, add CD FortiCHO basal medium or TFS-RDMP-1 basal medium or TFS-RDMP-9 basal medium or Hycell CHO with 25% of the total volume of the basal medium on the first day and the second day of culture respectively Basal medium.

[0041] 2. Optimization and screening of concentrated medium fed batch experiment methods

[0042] Concentrated Medium Feed Batch Experimental Method: Add 10% CHO CD Efficient Feed on the 3rd day of culture TM A and CHO CD Efficient Feed TM Mixture of B (the volume ratio of the two is 1:1), add 12.5% ​​CHO CD Efficient Feed on the 5th day TM A. CHO CD Efficient Feed ...

Embodiment 2

[0050] 1. Cell line B was used for expression and culture of recombinant human tumor necrosis factor receptor-Fc recombinant protein (rhTNFR-Fc) antibody

[0051] Table 3, Table 4, image 3 and Figure 4 Cell line B-CD FortiCHO, Cell line B-TFS-RDMP-1, Cell line B-TFS-RDMP-9 and Cell line B-Hycell CHO refer to CD FortiCHO basal medium with 50% of the total volume of basal medium For inoculation, add CD FortiCHO basal medium or TFS-RDMP-1 basal medium or TFS-RDMP-9 basal medium or Hycell CHO with 25% of the total volume of the basal medium on the first day and the second day of culture respectively Basal medium.

[0052] According to the method of Example 1, cell line B was used to express recombinant human tumor necrosis factor receptor-Fc recombinant protein (rhTNFR-Fc) antibody.

[0053] The cell viability and viable cell density of cell line B in different basal medium feeding methods are shown in Tables 3 and 4, in which different basal medium feeding methods have no si...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of cell culture, and discloses a CHO (Chinese hamster ovary) cell culture technology capable of reducing content of acidic variants. According to the invention, different basal culture media and concentrated culture media are added in the culture process of the CHO cell technology to increase the expression quantity of recombinant protein and reduce the acid peak level and further increase the quality, so that the technology is applicable to mass production and commercial application of antibody medicines and has better economic benefit.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a cell culture process of a recombinant human tumor necrosis factor receptor-Fc fusion protein based on a CHO expression system. Background technique [0002] At present, the mammalian expression system is the best system for heterologous expression of recombinant proteins. The most representative one is Chinese hamster ovary cell (CHO), which has the following advantages: it can be cultured in suspension, easy to operate, and easy to scale up; The expression ability, and the secreted protein has the correct post-translational modification function; only the self-protein is secreted, which is conducive to the purification of the product. Large-scale animal cell suspension culture has become one of the core technologies of biopharmaceuticals as an important means of commercially producing recombinant proteins and other proteins that require glycosylation modification and complex struct...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10
Inventor 黎美香鄢成伟杨彬孙文正李文佳倪健苏彦景柯军
Owner SUNSHINE LAKE PHARM CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com