Helicobacter pylori antibody detection kit, detection method and application

A technology for detecting Helicobacter pylori and antibodies, which is applied to measuring devices, analyzing materials through chemical reactions, and instruments. Accuracy of results, faster detection speed, and simplified detection process

A technology for detecting Helicobacter pylori and antibodies, which is applied to measuring devices, analyzing materials through chemical reactions, and instruments. Accuracy of results, faster detection speed, and simplified detection process

CN104614522AInactive Publication Date: 2015-05-13SHENZHEN NEW INDS BIOMEDICAL ENG
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  • Helicobacter pylori antibody detection kit, detection method and application
  • Helicobacter pylori antibody detection kit, detection method and application
  • Helicobacter pylori antibody detection kit, detection method and application

Examples

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Embodiment 1

[0247] A detection kit for measuring H.pylori IgG, comprising the following components:

[0248] Magnetic microsphere solution coated with HP antigen

[0249] HP antigen concentration: 50μg / l Magnetic microsphere concentration: 1.2mg / ml

[0250] ABEI solution of labeled anti-human IgG antibody

[0251] Anti-human IgG antibody concentration: 50μg / l ABEI concentration: 0.5mg / l

[0252] Low point calibrator containing HP IgG, where HP IgG concentration: 10.573EIU

[0253] High-point calibrator containing HP IgG, wherein HP IgG concentration: 68.766EIU.

[0254] In actual implementation, each of the above components may contain BSA and preservatives, the concentration of BSA is 0.05g / ml, and sodium azide is used as the preservative, and the concentration is 0.02g / ml.

[0255] A detection method for H.pylori IgG, comprising the steps of:

[0256] Add samples and calibrators to the cuvette;

[0257] Add 100 μl of buffer solution, 20 μl of HP antigen-coated magnetic microsphere...

Embodiment 2

[0264] A detection kit for measuring H.pylori IgM, comprising the following components:

[0265] Magnetic microsphere solution coated with HP antigen

[0266] HP antigen concentration: 50μg / l Magnetic microsphere concentration: 1.2mg / ml

[0267] ABEI solution of labeled anti-human IgM antibody

[0268] Anti-human IgM antibody concentration: 50μg / l ABEI concentration: 0.5mg / l

[0269] Low point calibrator containing HP IgM, where HP IgM concentration: 10.573EIU

[0270] High point calibrator containing HP IgM, where HP IgM concentration: 68.766EIU.

[0271] In actual implementation, each of the above components may contain BSA and preservatives, the concentration of BSA is 0.05g / ml, and sodium azide is used as the preservative, and the concentration is 0.02g / ml.

[0272] A detection method for H.pylori IgM, comprising the steps of:

[0273] Add samples and calibrators to the cuvette;

[0274] Add 100 μl of buffer solution, 20 μl of HP antigen-coated magnetic microsphere s...

Embodiment 3

[0281] A detection kit for measuring H.pylori IgA, comprising the following components:

[0282] Magnetic microsphere solution coated with HP antigen

[0283] HP antigen concentration: 50μg / l Magnetic microsphere concentration: 1.2mg / ml

[0284] ABEI solution of labeled anti-human IgA antibody

[0285] Anti-human IgA antibody concentration: 50μg / l ABEI concentration: 0.5mg / l

[0286] Low point calibrator containing HP IgA, where HP IgA concentration: 10.573EIU

[0287] High point calibrator containing HP IgA, HP IgA concentration: 68.766EIU.

[0288] In actual implementation, each of the above components may contain BSA and preservatives, the concentration of BSA is 0.05g / ml, and sodium azide is used as the preservative, and the concentration is 0.02g / ml.

[0289] A detection method for H.pylori IgA, comprising the steps of:

[0290] Add samples and calibrators to the cuvette;

[0291] Add 100 μl of buffer solution, 20 μl of HP antigen-coated magnetic microsphere solutio...

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Abstract

The invention discloses a helicobacter pylori antibody detection kit, comprising the following constituents: a magnetic microsphere solution directly coating or indirectly connecting helicobacter pylori antigen; and a marking tracer solution directly coating or indirectly connecting anti-human IgG antibody; and / or a marking tracer solution directly coating or indirectly connecting anti-human IgM antibody; and / or a marking tracer solution directly coating or indirectly connecting anti-human IgA antibody; and / or a marking tracer solution directly coating or indirectly connecting staphylococcus aureus A protein. The invention further discloses a corresponding helicobacter pylori antibody detection method and application thereof. The kit, the method and the application provided by the invention can detect various antibodies, including helicobacter pylori IgG antibody, helicobacter pylori IgM, helicobacter pylori IgA and total helicobacter pylori antibody, are applicable to complex conditions of helicobacter pylori infected person better, moreover, can accurately detect the helicobacter pylori antibody without pre-dilution to samples.

Description

technical field [0001] The invention relates to chemiluminescent immunodetection technology, in particular to a helicobacter pylori antibody chemiluminescent immunodetection kit, detection method and application. Background technique [0002] In 1982, Australian scholar Marshall observed a bacterium in the gastric mucosa that was associated with the onset of chronic gastric disease. In 1989, Goodwin et al. named it Helicobacter pylori (HP), which was recognized by the academic community. In 1994, the World Health Organization / International Agency for Research on Cancer (WHO / IARC) designated Helicobacter pylori as a human I carcinogen. Helicobacter pylori is a unipolar, multi-flagellate, blunt-rounded, spirally curved bacterium with a length of 2.5-4.0 μm and a width of 0.5-1.0 μm. It often presents a typical spiral or arc on the surface of gastric mucosal epithelial cells; When growing on solid media, in addition to the typical morphology, sometimes rod or spherical shapes...

Claims

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Application Information

Patent Timeline
13 May 2015
Publication
CN104614522A
IPC
G01N33/558; G01N21/64
CPC
G01N33/56911; G01N21/76
Inventors
饶微; 孙普