Breeding method of a high-quality disease-resistant light-insensitive kenaf cytoplasmic male sterile line
A male sterile line, light insensitivity technology, applied in the field of crop genetics and breeding, can solve problems such as fiber yield loss, fiber yield reduction, etc., and achieve the effect of improving comprehensive utilization, excellent fiber quality, and increasing farmers' income
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Embodiment 1
[0020] (1) Introduce kenaf germplasm resources, and sow into rows according to species under long-day conditions (planting 10 plants on average in each row). Each strain of the seven-lobed-leaf kenaf was treated with black cloth for short-day shading treatment (lighting duration = 12.5h, internal light intensity during shading = 45LUX) for 30 days, and the light-insensitive variety Fuhonghang 992B and the light-sensitive variety Fuhonghang 992B were set. Red 952 was used as a control. Eliminate early-flowering lines due to short-day shading treatment, identify the light-insensitive kenaf germplasm resource Zanyin No. Test and restore the relationship, and confirm that Zanyin No. 1 is the maintenance material;
[0021] (2) The light-insensitive variety Zanyin No. 1 was used as the female parent, and the high anthracnose-resistant variety Fuhong No. 3 (variety a) and the high-quality variety Fuhong No. 7 (variety b) were used as the male parents to obtain 2 hybrids. Hybrid F ...
Embodiment 2
[0031] (1) Sow the self-fertilized kenaf progeny lines according to the variety under long-day conditions (15 plants are evenly planted in each row). Black cloth was used for short-day shading treatment (light duration = 12.5h, internal light intensity during shading = 45LUX) for 30 days, and the light-insensitive variety Zanyin No. 1 and the light-sensitive variety Fuhong 952 were used as controls. We eliminated the early-flowering strains due to short-day shading treatment, and identified the light-insensitive kenaf variety Fuhonghang 1 with growth characteristics comparable to the light-insensitive variety Zanyin 1, and used the cytoplasmic male sterile line Fuhong 952A for preliminary testing Restore the relationship and confirm that Fuhonghang No. 1 is the maintenance material;
[0032] (2) Using the light-insensitive variety Fuhonghang No. 1 as the female parent, crossed with the high anthracnose-resistant variety Fuhong No. 3 (variety a) and the high-quality variety Fuh...
Embodiment 3
[0042] (1) Sow the self-fertilized kenaf progeny lines according to the variety under long-day conditions (10 plants are evenly planted in each row). When the kenaf grows to seven-lobed leaves, each The strains were treated with black cloth for short-day shading (light duration = 12.5h, light intensity during shading = 45LUX) for 30 days, and the light-insensitive variety Zanyin No. 1 and the light-sensitive variety Fuhong 952 were set as controls. The early flowering lines due to short-day shading treatment were eliminated, and the light-insensitive kenaf variety Fuhonghang 952, whose growth characteristics were comparable to the light-insensitive variety Zanyin No. 1, was identified, and the cytoplasmic male sterile line Fuhong 952A was used for initial testing and recovery. Guarantee relationship, confirm Fuhonghang 952 as maintenance material;
[0043] (2) The light-insensitive variety Fuhonghang 952 was used as the female parent, and the high-anthracnose-resistant variety...
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