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Culturing method of microalgae and culture medium thereof

A technology for microalgae cultivation and culture medium, which is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., and can solve the problems of unfavorable microalgae growth, limited microalgae production and oil accumulation, and long microalgae cultivation cycle. And other issues

Inactive Publication Date: 2015-05-20
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the two-step method used in the prior art to accumulate oil makes the entire microalgae culture cycle longer, and the conditions for the massive proliferation of microalgae are not suitable for accumulating oil, and stress culture conditions such as high light intensity or high salinity or lack of nitrogen and phosphorus etc. are not conducive to the growth of microalgae, resulting in the limitation of microalgae production and oil accumulation

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  • Culturing method of microalgae and culture medium thereof
  • Culturing method of microalgae and culture medium thereof
  • Culturing method of microalgae and culture medium thereof

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specific Embodiment approach

[0053] Hereinafter, the embodiments of the present invention respectively set up two groups of comparative examples, examples and experimental examples to illustrate the present invention. These examples are merely examples for specifically illustrating the present invention, and those skilled in the art will understand that the scope of the present invention is not limited by these examples, comparative examples, and test examples.

[0054] It should be noted that the process of obtaining algae species may also be included before culturing microalgae in each group of control examples and examples, which is not limited here. Algae species can be obtained through commercial means, and a large number of algae species can be obtained quickly by culturing in full medium for 7-9 days.

[0055] After obtaining the algae species, the process of screening the algae species can also be included, specifically: centrifuge an appropriate amount of algae species for 5min-10min, discard the...

Embodiment 1

[0070] The medium A was used in the embodiment 1, wherein the initial concentrations of nitrogen and phosphorus were 10 mg / L and 3 mg / L respectively.

[0071] Nannochloropsis pseudochloropsis was inoculated into the medium A for cultivation, and three parallels were established, and the specific steps were as follows:

[0072] Inoculation process:

[0073] Dilute the screened Nannochloropsis pseudochloropsis with the medium A, and inoculate it into a 5 L plate reactor 1 with a light path of 3 cm, so that the OD value of the algae liquid is 2.5.

[0074] One-step microalgae cultivation process:

[0075] Prepare a phosphorus solution of a certain concentration, calculate the speed of continuously adding phosphorus to the medium A according to the speed of phosphorus consumption in the microalgae culture process and the concentration of the phosphorus solution, and pass through the medium A at this speed The peristaltic pump (not shown in the figure) continuously adds the phosp...

Embodiment 2

[0079] The medium B was used in the embodiment 2, and the concentrations of nitrogen and phosphorus were 15 mg / L and 4 mg / L respectively.

[0080] Nannochloropsis was inoculated into the medium B for cultivation, and three parallels were set up, the steps were as follows:

[0081] Inoculation process:

[0082] The inoculation process is basically the same as in Example 1, and will not be repeated here.

[0083] One-step microalgae cultivation process:

[0084] Prepare a nitrogen solution with a certain concentration, calculate the speed of continuously adding nitrogen to the medium B according to the speed of nitrogen consumption during the microalgae cultivation process and the concentration of the nitrogen solution, and pass through the medium B at this speed The peristaltic pump (not shown in the figure) continuously adds the nitrogen solution, so that the concentration of nitrogen is always kept within 10-15mg / L, and a certain concentration of phosphorus solution is prep...

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Abstract

The invention relates to the field of microalgae culturing and particularly relates to a culturing method of the microalgae and a culture medium. The culture medium is capable of increasing the yield and the lipid accumulation of the microalgae, overcoming the shortcomings of the limited increasing of the yield and the lipid accumulation of the microalgae due to the mutual restriction of the yield and the lipid accumulation of the microalgae in the prior art. The embodiment of the invention provides a culturing method of the microalgae. The method includes the following steps: culturing the microalgae by the culture medium, in the microalgae culturing process, keeping the nitrogen concentration of the culture medium in a first specified range and keeping the phosphorus concentration in a second specified range, wherein the upper limit of the first specified range is less than the lower limit of the nitrogen concentration of the whole culture medium, and the upper limit of the second specified range is less than the lower limit of the phosphorus concentration of the whole culture medium.

Description

technical field [0001] The invention relates to the field of microalgae cultivation, in particular to a microalgae cultivation method and a culture medium thereof. Background technique [0002] Microalgae cells contain a variety of high-value nutrients and chemical raw materials. Cell metabolism produces polysaccharides, oils, proteins, pigments, etc., which are widely used in the pharmaceutical industry, food industry, environmental testing and purification, biotechnology and renewable energy manufacturing Widely used, among them, the oil in microalgae is equivalent to petroleum as a renewable energy source. This biological "crude oil" is quite environmentally friendly and can be used to refine gasoline, diesel, aviation fuel, and can be used as raw materials for plastic products and medicines. One of the most promising energy sources. [0003] In order to solve the current problems of energy shortage and long regeneration cycle of renewable energy, in order to increase th...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 许永白雪梅马卫敬王慧岭蔡忠贞桑俊宝程小雪滕杰
Owner ENN SCI & TECH DEV
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