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Environment-friendly and non-toxic hemolytic agent

A hemolytic agent and environmentally friendly technology, applied in the field of blood analysis, can solve problems such as poor dispersion, achieve low cracking ability, enhance stability, and reduce operating time

Inactive Publication Date: 2015-05-20
韩冰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Chinese patent CN1866011A discloses an environmentally friendly cyanide-free hemolytic agent, which uses sodium dihydrogen phosphate as a buffer solution and dodecyltrimethylammonium chloride to form a storage solution. The hemolytic agent uses water as a solvent, and its dispersibility after hemolysis Poor and counted for triplicates

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  • Environment-friendly and non-toxic hemolytic agent

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Comparison scheme
Effect test

Embodiment 1

[0013] The hemolytic agent A is composed of the following components by weight: 35 parts of sodium dodecylsulfonate, 3 parts of sodium edetate, 3 parts of citric acid, 2 parts of sodium chloride, 78 parts of dimethyl carbonate share;

[0014] Described buffer solution is 20 parts of sodium acetate, 60 parts of glacial acetic acid.

[0015] Preparation method: Weigh dimethyl carbonate in parts by weight, then sodium dodecylsulfonate, sodium ethylenediamine tetraacetate and sodium chloride in parts by weight, after completely dissolving, add citric acid and the pH value is about 2.8, Prepare hemolytic agent A; weigh parts by weight of sodium acetate and glacial acetic acid, and mix them uniformly to obtain a buffer solution.

Embodiment 2

[0017] The hemolytic agent A is composed of the following components by weight: 30 parts of sodium dodecylsulfonate, 4 parts of sodium edetate, 2 parts of citric acid, 1.5 parts of sodium chloride, 65 parts of dimethyl carbonate share;

[0018] Described buffer solution is 20 parts of sodium acetate, 55 parts of glacial acetic acid.

[0019] Preparation method: Weigh dimethyl carbonate in parts by weight, then sodium dodecylsulfonate, sodium ethylenediamine tetraacetate and sodium chloride in parts by weight, after completely dissolving, add citric acid, and the pH value is about 2.6, Prepare hemolytic agent A; weigh parts by weight of sodium acetate and glacial acetic acid, and mix them uniformly to obtain a buffer solution.

Embodiment 3

[0021] The hemolytic agent A is composed of the following components by weight: 38 parts of sodium dodecylsulfonate, 2 parts of sodium edetate, 4.5 parts of citric acid, 3 parts of sodium chloride, 80 parts of dimethyl carbonate share;

[0022] Described buffer solution is 20 parts of sodium acetate, 50 parts of glacial acetic acid.

[0023] Preparation method: Weigh dimethyl carbonate in parts by weight, then sodium dodecylsulfonate, sodium ethylenediamine tetraacetate and sodium chloride in parts by weight, after completely dissolving, add citric acid and the pH value is about 2.4, Prepare hemolytic agent A; weigh parts by weight of sodium acetate and glacial acetic acid, and mix them uniformly to obtain a buffer solution.

[0024] The method of using the hemolytic agent of the present invention: After adding the hemolytic agent A to the blood sample to be tested, the buffer solution can be added after a period of time.

[0025] Comparative analysis: If the solvent dimethy...

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Abstract

The invention belongs to the field of blood analysis, and particularly relates to an environment-friendly and non-toxic hemolytic agent which comprises a hemolytic agent A and a buffer solution, wherein the hemolytic agent A comprises 30-40 parts of sodium dodecyl sulfate, 2-4 parts of sodium ethylene diamine tetracetate, 2-5 parts of citric acid, 1-3 parts of sodium chloride and 60-80 parts of dimethyl carbonate; the buffer solution comprises 20 parts of sodium acetate and 50-65 parts of glacial acetic acid. The hemolytic agent is environmentally friendly and non-toxic, free from damage to a blood analysis instrument and high in accuracy.

Description

technical field [0001] The invention belongs to the field of blood analysis, and in particular relates to an environment-friendly and non-toxic hemolytic agent. Background technique [0002] Blood cell analysis is the most commonly used clinical laboratory detection index, which has been rapidly applied and popularized. In blood cell analysis, hemolytic agents are required when using blood analysis devices to classify white blood cells. Hemolytic agents have the ability to destroy the structure of blood cells, so as to achieve the purpose of hemolysis. Hemolytic agents can destroy red blood cells and eliminate their interference on white blood cell analysis. [0003] Chinese patent CN1866011A discloses an environmentally friendly cyanide-free hemolytic agent, which uses sodium dihydrogen phosphate as a buffer solution and dodecyltrimethylammonium chloride to form a storage solution. The hemolytic agent uses water as a solvent, and its dispersibility after hemolysis Poor and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48
Inventor 韩冰
Owner 韩冰