Flow cytometer

A flow cytometer and sheath flow technology, applied in the field of flow cytometry, can solve the problems of infeasibility of multi-color fluorescence detection, limited effective use of spatial filters, poor background light recognition ability, etc.

Active Publication Date: 2015-05-20
IRIS INT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0031] 1. Limits the effective use of spatial filters; and
[0032] 2. Poor background light recognition ability
Small-area APDs are considered infeasible for multicolor fluorescence detection applications since efficient color separation can only be accomplished economically with collimated beams

Method used

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Embodiment Construction

[0144] Flow cytometer

[0145] figure 1 Shown is a flow cytometer generally identified by reference numeral 40 according to the present disclosure. The flow cytometer 40 includes:

[0146] 1. LD-based optical subsystem 50;

[0147] 2. Compound microscope objective lens 60;

[0148] 3. Fluid subsystem 70 for supplying liquid sheath flow;

[0149] 4. A peristaltic pump 80, which is used to inject a liquid sample stream containing particles to be analyzed into the liquid sheath stream supplied by the fluid subsystem 70. The liquid sample stream is composed of the liquid sheath flowing through the observation area. Dynamic focusing, in which the compound microscope objective lens 60 collects and images the light scattered and / or the fluorescence emitted by the particles in the observation area;

[0150] 5. An optical fiber 852, which receives the light scattered and / or the fluorescence emitted by the particles in the observation area collected and imaged by the compound microscope objecti...

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Abstract

The disclosed flow cytometer (40) includes; 1. a laser diode ("LD") based optical subsystem (50) for impinging a beam of light upon particles passing through a viewing zone; 2. a composite microscope objective (60) for gathering and imaging light scattered from or fluoresced by particles passing through the viewing zone; 3. a fluidic subsystem (70) for supplying a liquid sheath flow to the viewing zone; 4. a peristaltic pump (80) for injecting into the liquid sheath flow a liquid sample flow carrying particles that passes together with the liquid sheath flow through the viewing zone; 5. a multimode optical fiber (852) that receives scattered and fluoresced light from the viewing zone that the composite microscope objective (60) gathers and images; and 6. a wavelength division multiplexer (90) for optically separating into color bands light received via the optical fiber (852).

Description

Technical field [0001] The present disclosure generally relates to the technical field of flow cytometry, and more specifically, to the structure and operation of an improved flow cytometer together with the various independent components included therein. Background technique [0002] Flow cytometry is a biophysical technique used in cell counting, sorting, biomarker detection, and protein engineering. In flow cytometry, cells suspended in a liquid stream pass through electronic detection equipment. Flow cytometry allows simultaneous multiparametric analysis of physical and / or chemical properties of up to thousands of cells per second. [0003] Flow cytometry has many applications including molecular biology, pathology, immunology, plant biology and marine biology. Flow cytometry is also widely used in medicine (especially in transplantation, hematology, tumor immunology and chemotherapy, prenatal diagnosis, genetics, and sperm sorting for gender preselection). In marine biolog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14
CPCG01N15/1434G01N15/1459G02B6/29365G01N2015/1006G02B6/4215G01N15/1436G01N2015/144G02B21/04
Inventor 陈永勤
Owner IRIS INT
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