Gerbera adventitious bud induction and plant regeneration method by using root as explant

An explant and bud induction technology, applied in the fields of botanical equipment and methods, plant regeneration, horticultural methods, etc., can solve the problem that the tissue culture of gerbera is still blank, and achieve accelerated asexual reproduction, a simple culture process, and no Effects of Seasonal Restrictions

Inactive Publication Date: 2015-05-27
SOUTHWEST FORESTRY UNIVERSITY
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Problems solved by technology

However, in many reports on in vitro culture, the tissue cult

Method used

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Examples

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Embodiment 1

[0014] Example 1: The adventitious bud induction and plant regeneration of gerbera were carried out in the laboratory of Forestry College of Southwest Forestry University and the Greenhouse, and the implementation time was 2013.

[0015] (1) Acquisition of explants: Aseptic bottle seedlings (variety name solar wind) propagated with gerbera filaments as explants are used as raw materials. This material is a degraded material with reduced flower shape and cannot be sold up to the standard. On the ultra-clean workbench, cut the sterile seedlings with a height of about 3 to 4 cm into individual seedlings, and inoculate them on the medium of 1 / 2MS + IBA 0.5 mg / L for rooting and sampling. Among them, pH 5.8, sucrose 20 g / L, agar 4 g / L. Culture conditions: The culture temperature is 21±2°C, and the light intensity is 1600-1800 Lux for 12 h / d. After 15 days of growth, the roots elongated to a certain length, and 0.8 cm long root segments were cut as explant materials.

[0016] (2) S...

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Abstract

The invention relates to a plant tissue culture propagation technology, more specifically, relates to a gerbera adventitious bud induction and plant regeneration method by using a root as an explant. The method is characterized by comprising the following steps: by using degraded gerbera root as the explant, proliferating and rooting culturing to obtain gerbera rooted seedling. The gerbera rooted seedling is stable in inheritable character, simple in culture process, easy to root, short in culture and propagation period, and easy to survive after being transplanted.

Description

technical field [0001] The invention relates to plant tissue culture propagation technology, more specifically, relates to a method for inducing adventitious buds of gerbera chrysanthemum with roots as explants and plant regeneration. Background technique [0002] Gerbera ( Gerbera jamesonii Bolus), also known as gerbera, sun flower, breviscapita, etc., is a perennial evergreen perennial herbaceous flower of the genus Gerbera in the family Asteraceae. It is native to Africa and is one of the top five cut flowers in the world. Gerbera has rich colors and huge flowers. It is an ideal material for artistic flower arrangement and has important ornamental value. It can bloom all year round under suitable cultivation conditions. It can be used as urban ornamental flowers and potted decorations. However, since gerbera is a cross-pollinated plant, the seedlings vary greatly, resulting in thinner pedicels, softer flowers, etc., which cannot maintain the characteristics of good vari...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 何承忠赵雁鸣李旦许昌慧吴英英辛培尧张斌
Owner SOUTHWEST FORESTRY UNIVERSITY
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