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A technology of probiotics and equipment, applied in the field of preparing probiotics, can solve the problems of expensive freeze dryer, loss of viable bacteria, high energy consumption, etc.
Active Publication Date: 2014-11-26
THANKCOME BIOLOGICAL SCI & TECH CO LTD
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Problems solved by technology
However, the freeze dryer is more expensive and consumes more energy during the drying process, so the processing cost is higher
[0011] When pulverizing the material obtained after freeze-drying, the pulverizer used in the existing pulverization process has the problem of uneven particle size after pulverization, so that the highly active bacteria cannot be fully screened out in the next sieving process, which greatly reduced productivity
[0012] When diluting and mixing the pulverized and sieved materials, the existing process adopts the method of spiral stirring, which has the problem of uneven stirring of the materials, resulting in the loss of some viable bacteria, which affects the final yield, and the cleaning of the propeller blades is also troublesome.
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Embodiment 1
[0073] (Example 1, equipment system for preparing probiotics)
[0074] The equipment system for preparing probiotics in this embodiment includes a fermenter 1 , a filtering device 2 , a freeze-drying device, a pulverizer 4 and a stirrer 5 .
[0075] See figure 1 , the fermenter 1 includes a tank body 11, a motor 12, an outer magnetic steel 13-1, an inner magnetic steel 13-2, a stirring shaft 14-1, a stirring blade 14-2, a beam 15, a bearing 16, a water inlet pipe 17, a bracket 18 and base 19.
[0076] The base 19 is placed on the ground, and the support 18 is fixed on the upper surface of the base 19 from above by its lower end, and the upper end of the support 18 is fixedly connected with the bottom of the tank body 11, so that the tank body 11 is positioned above the base 19 and is held by the support 18. The fixed support is separated from the upper surface of the base 19 .
[0077] Tank body 11 comprises tank main body 11-1 and loam cake 11-2, and the upper end of tank...
Embodiment 2
[0119] (Example 2, the method for preparing probiotics)
[0120] The method for preparing probiotics in this embodiment adopts the equipment system of Embodiment 1.
[0121] The method for preparing probiotics comprises the following steps:
[0122] ①Prepare the culture medium and sterilize it at 121°C for 15 minutes; the formulations of the culture medium of Lactobacillus bulgaricus and Streptococcus thermophilus prepared in this example are as follows: 10wt% skim milk powder, 2.5wt% whey powder, lactose 2 wt %, yeast extract 1.5 wt %, vitamin C 0.5 wt % and the balance of purified water.
[0123] ②Inoculation, pour the culture solution prepared in step ① into the fermenter 1, mix Lactobacillus bulgaricus and Streptococcus thermophilus at the ratio of initial bacteria number of 1:1, inoculate into the medium, Lactobacillus bulgaricus and Streptococcus thermophilus Cocci accounted for 1% by weight of the inoculated material.
[0124] ③ Fermentation, culture the material ino...
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Abstract
The invention discloses a method for preparing probiotics. An equipment system for preparing probiotics used by the preparation method comprises a fermentation tank, a filter device, a freeze-drying device, a pulverizator and a stirrer, wherein the freeze-drying device comprises a cryogenic refrigerator and a freeze dryer; a temperature sensor of the freeze dryer is fixed on the lateral wall of a material tray, and positioned at the middle position in the up-and-down direction of the lateral wall of the material tray; a probe end of the temperature sensor is arranged inside the material tray; one end, facing to the outer side of the material tray, of the temperature sensor is connected with a cable; the other end of the cable is connected with a cable joint and then connected with a joint of a temperature measurement and control system of the freeze dryer. The preparation method comprises the steps of preparing a culture solution, sterilizing, inoculating, filtering and concentrating, carrying out freeze drying, crushing and mixing. The freezing step of the material is carried out in the cryogenic refrigerator when the probiotics are prepared while the drying process of the material is carried out in the freeze-drying device, so that energy consumption is reduced; the production efficiency is improved.
Description
[0001] This application is a divisional application of the invention patent application with the application number 201110459650.9 and the application date is December 31, 2011, and the invention name is "fermentation tank, equipment system for preparing probiotics and method for preparing probiotics". technical field [0002] The invention relates to a method for preparing probiotics. Background technique [0003] Probiotics refer to microorganisms that have medical and health effects on human and animal bodies by improving the balance of microorganisms and enzymes in the body, stimulating specific or non-specific immune mechanisms. [0004] At present, there are two main production processes of probiotic products, namely solid fermentation method and large tank liquid submerged fermentation method. The solid fermentation method is a fermentation method in which probiotics are inoculated on a solid medium for fermentation and cultivation. At present, most probiotic products...
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