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Method for determining content of platycodon grandiflorum

A measurement method, the technology of platycodon, applied in the field of assaying platycodon content, can solve problems such as unqualified content

Inactive Publication Date: 2015-05-27
吉林敖东中药饮片股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for determining the content of bellflower, aiming to solve the problem that the content of bellflower is often unqualified according to the method of Pharmacopoeia

Method used

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  • Method for determining content of platycodon grandiflorum

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Experimental program
Comparison scheme
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Embodiment 1

[0019] Determination of content, according to high performance liquid chromatography determination:

[0020] Chromatographic conditions and system suitability test, using octadecylsilane bonded silica gel as filler; acetonitrile-water (25:75) as mobile phase; detection by evaporative light scattering detector. The number of theoretical plates should not be less than 3000 based on the calculation of Platycodon saponin D peak.

[0021] To prepare the reference substance solution, take an appropriate amount of the platycodon saponin D reference substance, weigh it accurately, add methanol to make a solution containing 0.5mg per 1ml, and obtain it.

[0022] The preparation of need testing solution, get about 2g of this product powder (through No. 2 sieve), accurately weigh, accurately add 50% methanol 50ml, weigh, ultrasonic treatment (power 250W, frequency 40kHz) 30 minutes, let cool, Weigh the weight again, make up the lost weight with 50% methanol, shake well, filter, accurate...

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Abstract

The invention discloses a method for determining the content of platycodon grandiflorum. The method comprises the following steps: filtering platycodon grandiflorum powder by utilizing a number-two screen, mixing 2g of filtered platycodon grandiflorum powder with 50ml of 50% methanol to obtain a mixture, carrying out ultrasonic treatment on the mixture for 30 minutes, cooling and complementing, filtering, and recovering a solvent until the solvent is completely recovered; extracting residues, washing the residues, drying an organic layer by distillation, dissolving the organic layer by utilizing methanol, separating by utilizing a silica gel column, eluting and separating for multiple times, collecting an elution solution, drying the elution solution by distillation, fixing the constant volume, filtering and finally obtaining a sample; and separating, and calculating the content of the platycodon grandiflorum under experimental conditions that a high-efficiency liquid-phase chromatograph adopts a chromatographic column Agilent TC-C18 5 micrometers, 4.6*150mm, the column temperature is 30 DEG C, the flow rate is 1.0ml / L, and the ratio of acetonitrile to water is 25: 75. After multiple tests, the content of the platycodon grandiflorum is subjected to the great influence of different silica gels, the content of the platycodon grandiflorum is very high by virtue of the extraction of the silica gel produced in Qingdao sea, the platycodon grandiflorum is generally qualified, the problem that the content of the existing platycodon grandiflorum cannot reach the standard can be effectively solved, and the test difficulty of medicine enterprises utilizing pharmacopeia can be solved.

Description

technical field [0001] The invention belongs to the technical field of medical material detection, and in particular relates to a method for measuring the content of bellflower. Background technique [0002] According to the method of the Pharmacopoeia, the content of bellflower is often unqualified. The method of Chinese Pharmacopoeia 2010 is often unqualified for the determination of bellflower content. After many experiments, the silica gel of column chromatography is the main factor affecting the content of bellflower. Contents of the invention [0003] The object of the present invention is to provide a method for determining the content of bellflower, aiming to solve the problem that the content of bellflower is often unqualified according to the method of pharmacopoeia. [0004] The present invention is achieved in this way, a kind of assay method of bellflower content, the assay method of this bellflower content comprises: [0005] Step 1: Pass 2 g of Platycodon g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 徐庆玲戴云宾郑奉梅王振楠刘鹏
Owner 吉林敖东中药饮片股份有限公司
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