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Fragrant sarcococca herb total alkaloids as well as preparation method and application thereof

A technology of total alkaloids and fragrance, applied in the field of medicine

Inactive Publication Date: 2015-06-03
FUDAN UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no reports about the very good inhibitory activity of the alkaloids of osmanthus osmanthus on a variety of tumor cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 extracts and separates the ethanol of osmanthus fragrans total alkaloids:

[0020] Sarcococca ruscifolia Stapf (Sarcococca ruscifolia Stapf) rhizome crushed (10.0g) was heated to reflux with 300ml of 95% ethanol for 3 times, the filtrate was combined, concentrated under reduced pressure to extract, suspended in 2% hydrochloric acid aqueous solution and extracted with petroleum ether to remove fat-soluble impurities, the acidic aqueous layer was adjusted to pH ~10 with NaOH, and then extracted with chloroform. The chloroform layer was dried over anhydrous sodium sulfate, and then concentrated under reduced pressure to obtain 0.15 g of alkaloid-containing fraction with a yield of 1.5%.

Embodiment 2

[0021] Embodiment 2 extracts and separates the sour water of the total alkaloids of Osmanthus osmanthus:

[0022] Sarcococca ruscifolia Stapf (Sarcococca ruscifolia Stapf) rhizome crushed (10.0g) was ultrasonically extracted with 300ml of 5% hydrochloric acid aqueous solution for 3 times, half an hour each time, the filtrate was combined and extracted with petroleum ether to remove fat-soluble impurities, and the pH of the acidic water layer was adjusted with NaOH to ~10, and then extracted with chloroform. The chloroform layer was dried over anhydrous sodium sulfate, and then concentrated under reduced pressure to obtain 0.12 g of alkaloid-containing fraction with a yield of 1.2%.

Embodiment 3

[0023] Embodiment 3 extracts and separates the sour water of the total alkaloids of Osmanthus osmanthus:

[0024] Sarcococca ruscifolia Stapf (Sarcococca ruscifolia Stapf) rhizome crushed (10.0g) was heated to reflux with 300ml of chloroform for 3 times, the filtrate was combined, concentrated to dryness under reduced pressure, suspended in 2% hydrochloric acid aqueous solution, extracted with petroleum ether, to remove fat-soluble impurities, acid The aqueous layer was adjusted to pH ~10 with NaOH and extracted with chloroform. The chloroform layer was dried over anhydrous sodium sulfate, and then concentrated under reduced pressure to obtain 0.10 g of alkaloid-containing fraction with a yield of 1.0%.

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Abstract

The invention belongs to the field of medicines, and relates to fragrant sarcococca herb total alkaloids and a preparation method therefore, and application of fragrant sarcococca herb total alkaloids in preparation of antitumor drugs. The fragrant sarcococca herb total alkaloids are prepared by screening natural antitumor products, and then extracting and separating fragrant sarcococca herb total alkaloids from alkaloid parts of buxaceae sarocococca fragrant sarcococca herb through acid water, chloroform, ethyl acetate, acetone, ethanol, methanol and other organic solvents; the test shows that fragrant sarcococca herb total alkaloids have obvious activity on habiting various tumor cells, so that the fragrant sarcococca herb total alkaloids can be further used for preparing the antitumor drugs for treating stomach cancer, lung cancer, colorectal cancer, cerebral cancer and breast cancer, and however, the drugs are not limited to the effects above; in addition, the drugs can be applied to the whole body or part of the body through a carrier or an excipient.

Description

technical field [0001] The invention belongs to the field of medicaments, and relates to total alkaloids of osmanthus fragrans and its preparation method and application in the preparation of antitumor drugs. Background technique [0002] It is known in the art that malignant tumors are still one of the major diseases that endanger people's health. At present, the treatment of tumors usually uses surgery, radiotherapy and chemotherapy. Among them, chemotherapy is one of the three traditional methods of tumor treatment. occupies an important position. The discovery of active ingredients from natural medicines has always been a hot spot in the research of anticancer drugs. [0003] Sarcococca ruscifolia Stapf, a Chinese herbal medicine, is rich in alkaloids. This plant is a folk medicine in my country and is mainly used to treat stomach problems, bruises, dizziness, palpitations, and sore throat. Over the years, the research on the activity of related steroidal alkaloids at ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P35/00A61K125/00
Inventor 俞培忠程科军石智胡昌奇
Owner FUDAN UNIV
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