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Separation tube

A separation tube and centrifuge tube technology, which is used in medical and high-risk areas where a large number of cell samples are separated

Inactive Publication Date: 2015-06-03
李小阁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] The invention provides a separation tube, which can overcome the existing problems and defects of existing products, has wider application, more convenient operation, safer product and high cell yield

Method used

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Examples

Experimental program
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Embodiment Construction

[0032] Specifically the implementation

[0033] This embodiment is based on the separation of isolated human peripheral blood lymphocytes

[0034] In this example, a 50ml separation tube was used to separate human peripheral blood lymphocytes. The lymphocyte separation medium was purchased domestically, with a density of 1.0770±0.001g / mL and an osmotic pressure of 290±15 mOsmol / kg.

[0035] 1. Take a 50ml sterile centrifuge tube and add 16ml of human peripheral blood lymphocyte separation medium;

[0036] 2. Insert the shelf (such as figure 1 shown, short corner up);

[0037] 2. When the partition has settled to the bottom, add 25ml of blood sample into the separation tube;

[0038] 3. Centrifuge, set the centrifugal force to 800 to 1000g, and the time to 20min to 30min;

[0039] 5. Stand still for 1~3min until the separator floats to the vicinity of the lymphocyte layer, then pour out the sample on the separator;

[0040] 6. Add about 20ml of normal saline to wash, and...

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Abstract

The invention discloses a separation tube device and an application method thereof. The separation tube device comprises a centrifuge tube and a partition. The partition is put in the centrifuge tube before being used, can separate conventional specimens and also can separate small-size specimens. Compared with the traditional separation tube, the separation tube device is easy to clean and less in chemical residue, can reduce the influence of material dissolution and separation liquid on separated cells, and can be widely applied to medical diagnosis, biological treatment and scientific research work.

Description

technical field [0001] The invention relates to a separator-type separation tube, which is mainly used in the field of biology, and is suitable for the separation of human and animal cells such as lymphocytes, white blood cells, and stem cells, and is especially suitable for high-risk medical technologies such as cell therapy and the separation of a large number of cell samples. field. Background technique [0002] Cell separation is one of the most commonly used separation methods in the fields of biology and medicine. It is often used in the performance research of specific cells, expanded culture, disease diagnosis and disease treatment. In particular, immune cell therapy technology has developed rapidly in recent years and has been widely used clinically. [0003] As early as 1992, biological cell immunotherapy was widely used clinically in the United States, and was officially approved by the US FDA. [0004] In March 2009, the Ministry of Health promulgated the "Admi...

Claims

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Application Information

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IPC IPC(8): C12M1/24C12M1/00
Inventor 李小阁
Owner 李小阁
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