Cell oxygen-deficient environment system constructed based on micro-numerical control living cell culture chamber

Abstract

The invention provides a cell oxygen-deficient environment system constructed based on a micro-numerical control living cell culture chamber. According to the invention, based on an existing living cell culture chamber, N2 is replaced by a mixed gas of O2 and N2, and the proportions of CO2 and the O2-N2 mixed gas are controlled by a numerical control device, so that the gas concentration of the culture chamber can be accurately controlled. Compared with existing oxygen-deficient incubators, the cell oxygen-deficient environment system provided by the invention is easy to operate and portable, can construct oxygen-deficient environments of different concentrations only by replacing an O2-N2 mixed gas compressed gas cylinder, also has high precision, can greatly lower the production cost, solves the defects of great consumption and low precision in existing oxygen-deficient incubators, and can autonomously control O2 concentration, and has wider application range.

Description

technical field [0001] The invention belongs to the technical field of biomedical experiments, and in particular relates to a cell anoxic environment system constructed on the basis of a miniature numerical control living cell culture chamber. Background technique [0002] o 2 It is an indispensable factor in maintaining cell life activities, and it is an indispensable participant in cellular aerobic respiration. Compared with the aerobic state, the transcription and expression of many genes can change to a certain extent in the cells in the hypoxic environment, such as glucose transporter-1 (GLUT-1), vascular endothelial growth factor (VEGF), Endothelin-1 (EDN-1), erythropoietin (EPO), oncogenes and tumor suppressor genes, etc. Therefore, studying the changes in the transcription and expression of related genes in cells in the hypoxic microenvironment has always been an important task in cell biology, molecular Hotspots in biology and life sciences. For example, in a hyp...

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Problems solved by technology

[0003] At present, the hypoxic incubator is mainly used for the hypoxic culture of cells or tissues. Although the hypoxic incubator has a large space, it has the following problems: 1) It is expensive to purchase and difficult to configure conventionally, which makes hypoxia-related research extremely difficult. Limited; 2) The incubator is large in size and difficult to move and carry; 3) Due to the large size of the incubator, it is difficult for N 2 , O 2 The consumption of compressed gas is huge, and the operating cost is high; 4) Maintenance and repair are inconvenient, which limits the application; 5) During the process of switching on and off the incubator, the anoxic environment in the box is broken, and it takes time to reach the corresponding level again. Hypoxic environment, causing the cells or tissues cultured in the hypoxic incubator to be in an intermittent reciprocating hypoxic environment, rather than a stable hypoxic environment; 6) The growth and movement of cells in the hypoxic environment cannot be monitored in real time, Real-time and dynamic information for cell research in hypoxic microenvironments is greatly limited

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