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One-dimensional cell growth method based on nano electrospinning

A growth method and electrospinning technology, which is applied in the field of one-dimensional cell growth based on nano-electrospinning, can solve the problems of high price and complicated operation, and achieve the effect of simple operation, environmental friendliness and low experimental cost

Active Publication Date: 2015-06-10
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for one-dimensional cell growth based on nano-electrospinning to solve the problems of complex operation and high price in the previous one-dimensional cell growth surface modification technology

Method used

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  • One-dimensional cell growth method based on nano electrospinning
  • One-dimensional cell growth method based on nano electrospinning
  • One-dimensional cell growth method based on nano electrospinning

Examples

Experimental program
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Embodiment 1

[0025] Prepare cured PDMS (polydimethylsiloxane) blocks without channel structures, soak them in PF127 solution (concentration: 0.2%) for 12 hours, rinse with deionized water and dry naturally, at this time, the surface of PDMS adsorbs The PF127 molecule inhibits cell attachment. Perform parallel electrospinning operation on the surface of PDMS modified by PF127, conduct parallel gelatin / PLGA electrospinning on its surface modification, fix the two ends of the electrospinning with new PDMS, and place the PDMS block in the ultra-clean bench for ultraviolet light Sterilize. Example 2

Embodiment 2

[0026] Validation of a nanoelectrospinning-based method for 1-dimensional growth of cells. The invention utilizes mesenchymal stem cells transfected with green fluorescent protein to investigate the one-dimensional growth surface of electrospinning cells. Such as figure 2 As shown, without PF127 treatment, PDMS surface was modified by parallel electrospinning, and cell culture was carried out. After 48 hours, the cells showed obvious orientation on the surface, but the cells were still attached to the entire bottom surface, and the degree of stretching was relatively small. Low. However, on the 1-dimensional growth surface of cells provided by the present invention, the cells are completely attached to the spinning and grow, and the cells become elongated due to full stretching, showing a typical 1-dimensional growth pattern ( image 3 ).

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Abstract

The invention provides a one-dimensional cell growth method based on nano electrospinning. The method comprises the following steps: preparing a cured PDMS (polydimethylsiloxane) block without a channel structure by utilizing a soft lithography method, soaking the block in a PF127 solution for more than 6 hours, carrying out washing with deionized water and then carrying out drying, wherein PF127 molecules adsorbed on the surface of the PDMS inhibit cells from adhering on walls at the time; and carrying out parallel silk electrospinning on the surface of the PDMS modified by the PF127 (Pluronic F-127), modifying the surface of the PDMS with parallel gelatin / PLGA electrospinning silk, and then placing the PDMS block in a super clean bench for ultraviolet sterilization. The sterilized PDMS block can be used for one-dimensional growth culture of cells. The method of the invention does not need expensive equipment, can realize one-shot large-area modification and has the advantages of simple and fast operation, low experimental cost, environment friendliness, etc.

Description

technical field [0001] The invention relates to the nanometer modification of the polymer surface and its application field, in particular to a one-dimensional cell growth method based on nanometer electrospinning. Background technique [0002] A cell's growth pattern is closely related to its behavior. According to different growth dimensions, it can be divided into 1-dimensional growth, 2-dimensional growth, and 3-dimensional growth. Among them, 2-dimensional growth is the most common way of cells in vitro, that is, cells grow on the bottom of the culture dish / flask in an adherent manner; 3-dimensional growth is the common growth mode of cells in organisms. In order to simulate the growth environment in vivo, cells The 3-dimensional culture in vitro has also attracted more and more people's attention; 1-dimensional growth is a special mode of existence of cells, which refers to the growth state of cells only adhering to a single fiber in the matrix, which is considered to...

Claims

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Application Information

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IPC IPC(8): C12N5/0775D01D5/00
Inventor 秦建华张旭马静云
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI